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Alternative SARS-CoV-2 detection protocol from self-collected saliva for mass diagnosis and epidemiological studies in low-incoming regions

Until mass vaccination befalls, control of the new betacoronavirus-associated severe acute respiratory syndrome pandemic (SARS-CoV-2) is based on decreasing virus circulation by social distancing and blocking transmission foci after diagnosis. Globally adopted SARS-CoV-2 diagnostic criteria embrace...

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Detalles Bibliográficos
Autores principales: de Oliveira, Luana Prado Rolim, Cabral, Aline Diniz, dos Santos Carmo, Andreia Moreira, Duran, Adriana Feliciano, Fermino, Diego Marin, Veiga, Glaucia Raquel Luciano, da Costa Aguiar Alves, Beatriz, Santana, Carla Moreira, Garcia, Felipe Baena, Santos, Edmar Silva, Jordão, Felipe Trovalim, Siqueira, Andressa Moreira, de Campos, Ivana Barros, Colpas, Daniela Rodrigues, Almeida, Fernanda Nascimento, Fonseca, Fernando Luiz Affonso, Sperança, Márcia Aparecida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626149/
https://www.ncbi.nlm.nih.gov/pubmed/34843823
http://dx.doi.org/10.1016/j.jviromet.2021.114382
Descripción
Sumario:Until mass vaccination befalls, control of the new betacoronavirus-associated severe acute respiratory syndrome pandemic (SARS-CoV-2) is based on decreasing virus circulation by social distancing and blocking transmission foci after diagnosis. Globally adopted SARS-CoV-2 diagnostic criteria embrace viral RNA detection by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) on nasopharynx secretions, which requires healthcare facilities and specialized personnel for sample collection. To develop an alternative protocol, hydrophilic cotton as the material and saliva as the source for biological sample collection in qRT-PCR/RT-endpoint-PCR SARS-CoV-2 diagnostic methods prepared with local consumables were evaluated using 99 archived nasopharynx samples previously diagnosed as positive for SARS-CoV-2 and 111 prospective saliva samples pared with nasopharynx samples from patients attending the local reference ABC Medical School diagnostic laboratory. The kappa agreement coefficient between the SARS-CoV-2 qRT-PCR and RT-endpoint-PCR was k = 0.97 (95 % CI 0.92−1.00) and k = 0.90 (95 % CI 0.81−0.99), respectively, on SARS-CoV-2-positive archived samples, with the initial qRT-PCR C(T) under 25. The agreement coefficient of the SARS-CoV-2 alternative saliva diagnostic protocol, when used to test the paired nasopharynx samples, was k = 0.79 (95 % CI 0.56–1,00). These data support that the SARS-CoV-2 diagnostic assay based on self-collected saliva on cotton represents an alternative protocol for mass diagnosis and epidemiological studies in low-income regions.