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Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress

Plant metal tolerance proteins (MTPs) play major roles in enhancing resistance to heavy metal tolerance and homeostasis. However, the role of MTPs genes in tomato, which is one of the most popular crops, is still largely limited. Hence, we investigated genome-wide study of tomato MTPs, including phy...

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Autores principales: El- Sappah, Ahmed H., Elrys, Ahmed S., Desoky, El-Sayed M., Zhao, Xia, Bingwen, Wang, El-Sappah, Hamza H., Zhu, Yumin, Zhou, Wanhai, Zhao, Xianming, Li, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626246/
https://www.ncbi.nlm.nih.gov/pubmed/34866994
http://dx.doi.org/10.1016/j.sjbs.2021.07.073
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author El- Sappah, Ahmed H.
Elrys, Ahmed S.
Desoky, El-Sayed M.
Zhao, Xia
Bingwen, Wang
El-Sappah, Hamza H.
Zhu, Yumin
Zhou, Wanhai
Zhao, Xianming
Li, Jia
author_facet El- Sappah, Ahmed H.
Elrys, Ahmed S.
Desoky, El-Sayed M.
Zhao, Xia
Bingwen, Wang
El-Sappah, Hamza H.
Zhu, Yumin
Zhou, Wanhai
Zhao, Xianming
Li, Jia
author_sort El- Sappah, Ahmed H.
collection PubMed
description Plant metal tolerance proteins (MTPs) play major roles in enhancing resistance to heavy metal tolerance and homeostasis. However, the role of MTPs genes in tomato, which is one of the most popular crops, is still largely limited. Hence, we investigated genome-wide study of tomato MTPs, including phylogenetic, duplication, gene structure, gene ontology and previous transcriptomic data analysis. Moreover, the MTPs expression behaviour under various heavy metals stress has rarely been investigated. In the current study, eleven MTP candidate genes were genome-wide identified and classified into three major groups; Mn-cation diffusion facilitators (CDFs), Fe/Zn-CDFs, and Zn-CDFs based on the phylogeny. Structural analysis of SlMTPs showed high gene similarity within the same group with cation_efflux or ZT_dimerdomains. Evolutionary analysis revealed that segmental duplication contributed to the expansion of the SlMTP family. Gene ontology further showed the vital roles of MTPs in metal-related processes. Tissue-specific expression profiling exhibited similar expression patterns in the same group, whereas gene expression varied among groups. The MTPs expression was evaluated after tomato treatments by five divalent heavy metals (Cd(2+), Co(2+), Mn(2+), Zn(2+,) and Fe(2+)). SlMTP genes displayed differential responses in either plant leaves or roots under heavy metals treatments. Nine and ten SlMTPs responded to at least one metal ion treatment in leaves and roots, respectively. In addition SlMTP1, SlMTP3, SlMTP4, SlMTP8, SlMTP10 and SlMTP11 exhibited the highest expression responses in most of heavy metals treatments. Overall, our findings presented a standpoint on the evolution of MTPs and their evolution in tomato and paved the way for additional functional characterization under heavy metal toxicity.
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spelling pubmed-86262462021-12-02 Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress El- Sappah, Ahmed H. Elrys, Ahmed S. Desoky, El-Sayed M. Zhao, Xia Bingwen, Wang El-Sappah, Hamza H. Zhu, Yumin Zhou, Wanhai Zhao, Xianming Li, Jia Saudi J Biol Sci Original Article Plant metal tolerance proteins (MTPs) play major roles in enhancing resistance to heavy metal tolerance and homeostasis. However, the role of MTPs genes in tomato, which is one of the most popular crops, is still largely limited. Hence, we investigated genome-wide study of tomato MTPs, including phylogenetic, duplication, gene structure, gene ontology and previous transcriptomic data analysis. Moreover, the MTPs expression behaviour under various heavy metals stress has rarely been investigated. In the current study, eleven MTP candidate genes were genome-wide identified and classified into three major groups; Mn-cation diffusion facilitators (CDFs), Fe/Zn-CDFs, and Zn-CDFs based on the phylogeny. Structural analysis of SlMTPs showed high gene similarity within the same group with cation_efflux or ZT_dimerdomains. Evolutionary analysis revealed that segmental duplication contributed to the expansion of the SlMTP family. Gene ontology further showed the vital roles of MTPs in metal-related processes. Tissue-specific expression profiling exhibited similar expression patterns in the same group, whereas gene expression varied among groups. The MTPs expression was evaluated after tomato treatments by five divalent heavy metals (Cd(2+), Co(2+), Mn(2+), Zn(2+,) and Fe(2+)). SlMTP genes displayed differential responses in either plant leaves or roots under heavy metals treatments. Nine and ten SlMTPs responded to at least one metal ion treatment in leaves and roots, respectively. In addition SlMTP1, SlMTP3, SlMTP4, SlMTP8, SlMTP10 and SlMTP11 exhibited the highest expression responses in most of heavy metals treatments. Overall, our findings presented a standpoint on the evolution of MTPs and their evolution in tomato and paved the way for additional functional characterization under heavy metal toxicity. Elsevier 2021-12 2021-08-02 /pmc/articles/PMC8626246/ /pubmed/34866994 http://dx.doi.org/10.1016/j.sjbs.2021.07.073 Text en © 2021 Published by Elsevier B.V. on behalf of King Saud University. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
El- Sappah, Ahmed H.
Elrys, Ahmed S.
Desoky, El-Sayed M.
Zhao, Xia
Bingwen, Wang
El-Sappah, Hamza H.
Zhu, Yumin
Zhou, Wanhai
Zhao, Xianming
Li, Jia
Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title_full Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title_fullStr Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title_full_unstemmed Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title_short Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title_sort comprehensive genome wide identification and expression analysis of mtp gene family in tomato (solanum lycopersicum) under multiple heavy metal stress
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626246/
https://www.ncbi.nlm.nih.gov/pubmed/34866994
http://dx.doi.org/10.1016/j.sjbs.2021.07.073
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