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Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders

Changes in the metabolic fingerprint of plasma during the onset of lay in broiler breeders were investigated. We used metabolomics to identify biomarkers of sexual maturity and to provide a comprehensive understanding of breeder metabolome during the pullet to hen transition period. A total of 36 pu...

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Autores principales: Afrouziyeh, Mohammad, Zukiwsky, Nicole M., Korver, Douglas R., Zuidhof, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626694/
https://www.ncbi.nlm.nih.gov/pubmed/34823168
http://dx.doi.org/10.1016/j.psj.2021.101532
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author Afrouziyeh, Mohammad
Zukiwsky, Nicole M.
Korver, Douglas R.
Zuidhof, Martin J.
author_facet Afrouziyeh, Mohammad
Zukiwsky, Nicole M.
Korver, Douglas R.
Zuidhof, Martin J.
author_sort Afrouziyeh, Mohammad
collection PubMed
description Changes in the metabolic fingerprint of plasma during the onset of lay in broiler breeders were investigated. We used metabolomics to identify biomarkers of sexual maturity and to provide a comprehensive understanding of breeder metabolome during the pullet to hen transition period. A total of 36 pullets were used, in which 30 pullets were randomly assigned to one of 10 unique growth trajectories and 6 birds were assigned to an unrestricted group. The growth trajectories were designed using a 3-phase Gompertz growth model with 10 levels of BW gain in the prepubertal and pubertal growth phases ranging from the breeder-recommended target BW to 22.5% higher, in 2.5% increments. The BW trajectories were applied to each individual bird using a precision feeding (PF) system, which collected BW and feed intake data for each individual bird. The birds were classified based on age at first egg (AFE), and 12 pullets were chosen from the lower and upper AFE extremes (early and late onset of lay) at 18, 20, 22, 24, and 26 wk of age to run repeated blood plasma metabolomic assays. The metabolomic profile data were collected using a direct-injection liquid chromatography-tandem mass spectrometry and steroid assays. Univariate analysis identified 87 differential metabolites between the early- and late-onset of lay groups at 24 wk of age and 104 differential metabolites between the pullet and hen groups. Further investigation of differential metabolites showed 15 potential biomarkers for pullet to hen transition by analyzing the receiver operating characteristic (ROC) curve, mainly consisting of carnitine and choline metabolites. Differential metabolites during the pullet to hen transition were mainly associated with lipid, energy, and amino acid metabolism pathways, which gave clues to the physiological and metabolic shifts resulting from sexual maturation. At 24 wk of age, the main pathways involved in differentiation of the early- and late-onset of lay groups were related to lipid and amino acid metabolism. These metabolites could be involved in biosynthesis of egg yolk precursors in the liver.
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spelling pubmed-86266942021-12-02 Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders Afrouziyeh, Mohammad Zukiwsky, Nicole M. Korver, Douglas R. Zuidhof, Martin J. Poult Sci METABOLISM AND NUTRITION Changes in the metabolic fingerprint of plasma during the onset of lay in broiler breeders were investigated. We used metabolomics to identify biomarkers of sexual maturity and to provide a comprehensive understanding of breeder metabolome during the pullet to hen transition period. A total of 36 pullets were used, in which 30 pullets were randomly assigned to one of 10 unique growth trajectories and 6 birds were assigned to an unrestricted group. The growth trajectories were designed using a 3-phase Gompertz growth model with 10 levels of BW gain in the prepubertal and pubertal growth phases ranging from the breeder-recommended target BW to 22.5% higher, in 2.5% increments. The BW trajectories were applied to each individual bird using a precision feeding (PF) system, which collected BW and feed intake data for each individual bird. The birds were classified based on age at first egg (AFE), and 12 pullets were chosen from the lower and upper AFE extremes (early and late onset of lay) at 18, 20, 22, 24, and 26 wk of age to run repeated blood plasma metabolomic assays. The metabolomic profile data were collected using a direct-injection liquid chromatography-tandem mass spectrometry and steroid assays. Univariate analysis identified 87 differential metabolites between the early- and late-onset of lay groups at 24 wk of age and 104 differential metabolites between the pullet and hen groups. Further investigation of differential metabolites showed 15 potential biomarkers for pullet to hen transition by analyzing the receiver operating characteristic (ROC) curve, mainly consisting of carnitine and choline metabolites. Differential metabolites during the pullet to hen transition were mainly associated with lipid, energy, and amino acid metabolism pathways, which gave clues to the physiological and metabolic shifts resulting from sexual maturation. At 24 wk of age, the main pathways involved in differentiation of the early- and late-onset of lay groups were related to lipid and amino acid metabolism. These metabolites could be involved in biosynthesis of egg yolk precursors in the liver. Elsevier 2021-10-12 /pmc/articles/PMC8626694/ /pubmed/34823168 http://dx.doi.org/10.1016/j.psj.2021.101532 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle METABOLISM AND NUTRITION
Afrouziyeh, Mohammad
Zukiwsky, Nicole M.
Korver, Douglas R.
Zuidhof, Martin J.
Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title_full Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title_fullStr Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title_full_unstemmed Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title_short Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title_sort plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
topic METABOLISM AND NUTRITION
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626694/
https://www.ncbi.nlm.nih.gov/pubmed/34823168
http://dx.doi.org/10.1016/j.psj.2021.101532
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