Is amplification bias consequential in transposon sequencing (TnSeq) assays? A case study with a Staphylococcus aureus TnSeq library subjected to PCR-based and amplification-free enrichment methods

As transposon sequencing (TnSeq) assays have become prolific in the microbiology field, it is of interest to scrutinize their potential drawbacks. TnSeq data consist of millions of nucleotide sequence reads that are generated by PCR amplification of transposon-genomic junctions. Reads mapping to the...

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Detalles Bibliográficos
Autores principales: Alkam, Duah, Wongsurawat, Thidathip, Nookaew, Intawat, Richardson, Anthony R., Ussery, David, Smeltzer, Mark S., Jenjaroenpun, Piroon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8627206/
https://www.ncbi.nlm.nih.gov/pubmed/34596508
http://dx.doi.org/10.1099/mgen.0.000655

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8627206/
https://www.ncbi.nlm.nih.gov/pubmed/34596508
http://dx.doi.org/10.1099/mgen.0.000655

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