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Differential nasopharyngeal microbiota composition in children according to respiratory health status

Acute respiratory infections (ARIs) constitute one of the leading causes of antibiotic administration, hospitalization and death among children <5 years old. The upper respiratory tract microbiota has been suggested to explain differential susceptibility to ARIs and modulate ARI severity. The aim...

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Autores principales: Henares, Desiree, Brotons, Pedro, de Sevilla, Mariona F., Fernandez-Lopez, Ana, Hernandez-Bou, Susanna, Perez-Argüello, Amaresh, Mira, Alex, Muñoz-Almagro, Carmen, Cabrera-Rubio, Raul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8627214/
https://www.ncbi.nlm.nih.gov/pubmed/34699345
http://dx.doi.org/10.1099/mgen.0.000661
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author Henares, Desiree
Brotons, Pedro
de Sevilla, Mariona F.
Fernandez-Lopez, Ana
Hernandez-Bou, Susanna
Perez-Argüello, Amaresh
Mira, Alex
Muñoz-Almagro, Carmen
Cabrera-Rubio, Raul
author_facet Henares, Desiree
Brotons, Pedro
de Sevilla, Mariona F.
Fernandez-Lopez, Ana
Hernandez-Bou, Susanna
Perez-Argüello, Amaresh
Mira, Alex
Muñoz-Almagro, Carmen
Cabrera-Rubio, Raul
author_sort Henares, Desiree
collection PubMed
description Acute respiratory infections (ARIs) constitute one of the leading causes of antibiotic administration, hospitalization and death among children <5 years old. The upper respiratory tract microbiota has been suggested to explain differential susceptibility to ARIs and modulate ARI severity. The aim of the present study was to investigate the relation of nasopharyngeal microbiota and other microbiological parameters with respiratory health and disease, and to assess nasopharyngeal microbiota diagnostic utility for discriminating between different respiratory health statuses. We conducted a prospective case–control study at Hospital Sant Joan de Deu (Barcelona, Spain) from 2014 to 2018. This study included three groups of children <18 years with gradual decrease of ARI severity: cases with invasive pneumococcal disease (IPD) (representative of lower respiratory tract infections and systemic infections), symptomatic controls with mild viral upper respiratory tract infections (URTI), and healthy/asymptomatic controls according to an approximate case–control ratio 1:2. Nasopharyngeal samples were collected from participants for detection, quantification and serotyping of pneumococcal DNA, viral DNA/RNA detection and 16S rRNA gene sequencing. Microbiological parameters were included on case–control classification models. A total of 140 subjects were recruited (IPD=27, URTI=48, healthy/asymptomatic control=65). Children’s nasopharyngeal microbiota composition varied according to respiratory health status and infection severity. The IPD group was characterized by overrepresentation of Streptococcus pneumoniae , higher frequency of invasive pneumococcal serotypes, increased rate of viral infection and underrepresentation of potential protective bacterial species such as Dolosigranulum pigrum and Moraxella lincolnii . Microbiota-based classification models differentiated cases from controls with moderately high accuracy. These results demonstrate the close relationship existing between a child’s nasopharyngeal microbiota and respiratory health, and provide initial evidence of the potential of microbiota-based diagnostics for differential diagnosis of severe ARIs using non-invasive samples.
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spelling pubmed-86272142021-11-29 Differential nasopharyngeal microbiota composition in children according to respiratory health status Henares, Desiree Brotons, Pedro de Sevilla, Mariona F. Fernandez-Lopez, Ana Hernandez-Bou, Susanna Perez-Argüello, Amaresh Mira, Alex Muñoz-Almagro, Carmen Cabrera-Rubio, Raul Microb Genom Research Articles Acute respiratory infections (ARIs) constitute one of the leading causes of antibiotic administration, hospitalization and death among children <5 years old. The upper respiratory tract microbiota has been suggested to explain differential susceptibility to ARIs and modulate ARI severity. The aim of the present study was to investigate the relation of nasopharyngeal microbiota and other microbiological parameters with respiratory health and disease, and to assess nasopharyngeal microbiota diagnostic utility for discriminating between different respiratory health statuses. We conducted a prospective case–control study at Hospital Sant Joan de Deu (Barcelona, Spain) from 2014 to 2018. This study included three groups of children <18 years with gradual decrease of ARI severity: cases with invasive pneumococcal disease (IPD) (representative of lower respiratory tract infections and systemic infections), symptomatic controls with mild viral upper respiratory tract infections (URTI), and healthy/asymptomatic controls according to an approximate case–control ratio 1:2. Nasopharyngeal samples were collected from participants for detection, quantification and serotyping of pneumococcal DNA, viral DNA/RNA detection and 16S rRNA gene sequencing. Microbiological parameters were included on case–control classification models. A total of 140 subjects were recruited (IPD=27, URTI=48, healthy/asymptomatic control=65). Children’s nasopharyngeal microbiota composition varied according to respiratory health status and infection severity. The IPD group was characterized by overrepresentation of Streptococcus pneumoniae , higher frequency of invasive pneumococcal serotypes, increased rate of viral infection and underrepresentation of potential protective bacterial species such as Dolosigranulum pigrum and Moraxella lincolnii . Microbiota-based classification models differentiated cases from controls with moderately high accuracy. These results demonstrate the close relationship existing between a child’s nasopharyngeal microbiota and respiratory health, and provide initial evidence of the potential of microbiota-based diagnostics for differential diagnosis of severe ARIs using non-invasive samples. Microbiology Society 2021-10-26 /pmc/articles/PMC8627214/ /pubmed/34699345 http://dx.doi.org/10.1099/mgen.0.000661 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Articles
Henares, Desiree
Brotons, Pedro
de Sevilla, Mariona F.
Fernandez-Lopez, Ana
Hernandez-Bou, Susanna
Perez-Argüello, Amaresh
Mira, Alex
Muñoz-Almagro, Carmen
Cabrera-Rubio, Raul
Differential nasopharyngeal microbiota composition in children according to respiratory health status
title Differential nasopharyngeal microbiota composition in children according to respiratory health status
title_full Differential nasopharyngeal microbiota composition in children according to respiratory health status
title_fullStr Differential nasopharyngeal microbiota composition in children according to respiratory health status
title_full_unstemmed Differential nasopharyngeal microbiota composition in children according to respiratory health status
title_short Differential nasopharyngeal microbiota composition in children according to respiratory health status
title_sort differential nasopharyngeal microbiota composition in children according to respiratory health status
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8627214/
https://www.ncbi.nlm.nih.gov/pubmed/34699345
http://dx.doi.org/10.1099/mgen.0.000661
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