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Removal of heavy oil from contaminated surfaces with a detergent formulation containing biosurfactants produced by Pseudomonas spp.
Industrial plants powered by heavy oil routinely experience problems with leaks in different parts of the system, such as during oil transport, the lubrication of equipment and mechanical failures. The surfactants, degreasing agents and solvents that make up detergents commonly used for cleaning gre...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8627655/ https://www.ncbi.nlm.nih.gov/pubmed/34900433 http://dx.doi.org/10.7717/peerj.12518 |
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author | Farias, Charles Bronzo B. Soares da Silva, Rita de Cássia F. Almeida, Fabíola Carolina G. Santos, Valdemir A. Sarubbo, Leonie A. |
author_facet | Farias, Charles Bronzo B. Soares da Silva, Rita de Cássia F. Almeida, Fabíola Carolina G. Santos, Valdemir A. Sarubbo, Leonie A. |
author_sort | Farias, Charles Bronzo B. |
collection | PubMed |
description | Industrial plants powered by heavy oil routinely experience problems with leaks in different parts of the system, such as during oil transport, the lubrication of equipment and mechanical failures. The surfactants, degreasing agents and solvents that make up detergents commonly used for cleaning grease-covered surfaces are synthetic, non-biodegradable and toxic, posing risks to the environment as well as the health of workers involved in the cleaning process. To address this problem, surfactant agents of a biodegradable nature and low toxicity, such as microbial surfactants, have been widely studied as an attractive, efficient solution to replace chemical surfactants in decontamination processes. In this work, the bacterial strains Pseudomonas cepacia CCT 6659, Pseudomonas aeruginosa UCP 0992, Pseudomonas aeruginosa ATCC 9027 and Pseudomonas aeruginosa ATCC 10145 were evaluated as biosurfactant producers in media containing different combinations and types of substrates and under different culture conditions. The biosurfactant produced by P. aeruginosa ATCC 10145 cultivated in a mineral medium composed of 5.0% glycerol and 2.0% glucose for 96 h was selected to formulate a biodetergent capable of removing heavy oil. The biosurfactant was able to reduce the surface tension of the medium to 26.40 mN/m, with a yield of approximately 12.00 g/L and a critical micelle concentration of 60.00 mg/L. The biosurfactant emulsified 97.40% and dispersed 98.00% of the motor oil. The detergent formulated with the biosurfactant also exhibited low toxicity in tests involving the microcrustacean Artemia salina and seeds of the vegetable Brassica oleracea. The detergent was compared to commercial formulations and removed 100% of the Special B1 Fuel Oil (OCB1) from different contaminated surfaces, demonstrating potential as a novel green remover with industrial applications. |
format | Online Article Text |
id | pubmed-8627655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86276552021-12-10 Removal of heavy oil from contaminated surfaces with a detergent formulation containing biosurfactants produced by Pseudomonas spp. Farias, Charles Bronzo B. Soares da Silva, Rita de Cássia F. Almeida, Fabíola Carolina G. Santos, Valdemir A. Sarubbo, Leonie A. PeerJ Biotechnology Industrial plants powered by heavy oil routinely experience problems with leaks in different parts of the system, such as during oil transport, the lubrication of equipment and mechanical failures. The surfactants, degreasing agents and solvents that make up detergents commonly used for cleaning grease-covered surfaces are synthetic, non-biodegradable and toxic, posing risks to the environment as well as the health of workers involved in the cleaning process. To address this problem, surfactant agents of a biodegradable nature and low toxicity, such as microbial surfactants, have been widely studied as an attractive, efficient solution to replace chemical surfactants in decontamination processes. In this work, the bacterial strains Pseudomonas cepacia CCT 6659, Pseudomonas aeruginosa UCP 0992, Pseudomonas aeruginosa ATCC 9027 and Pseudomonas aeruginosa ATCC 10145 were evaluated as biosurfactant producers in media containing different combinations and types of substrates and under different culture conditions. The biosurfactant produced by P. aeruginosa ATCC 10145 cultivated in a mineral medium composed of 5.0% glycerol and 2.0% glucose for 96 h was selected to formulate a biodetergent capable of removing heavy oil. The biosurfactant was able to reduce the surface tension of the medium to 26.40 mN/m, with a yield of approximately 12.00 g/L and a critical micelle concentration of 60.00 mg/L. The biosurfactant emulsified 97.40% and dispersed 98.00% of the motor oil. The detergent formulated with the biosurfactant also exhibited low toxicity in tests involving the microcrustacean Artemia salina and seeds of the vegetable Brassica oleracea. The detergent was compared to commercial formulations and removed 100% of the Special B1 Fuel Oil (OCB1) from different contaminated surfaces, demonstrating potential as a novel green remover with industrial applications. PeerJ Inc. 2021-11-25 /pmc/articles/PMC8627655/ /pubmed/34900433 http://dx.doi.org/10.7717/peerj.12518 Text en © 2021 Farias et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biotechnology Farias, Charles Bronzo B. Soares da Silva, Rita de Cássia F. Almeida, Fabíola Carolina G. Santos, Valdemir A. Sarubbo, Leonie A. Removal of heavy oil from contaminated surfaces with a detergent formulation containing biosurfactants produced by Pseudomonas spp. |
title | Removal of heavy oil from contaminated surfaces with a detergent formulation containing biosurfactants produced by Pseudomonas spp. |
title_full | Removal of heavy oil from contaminated surfaces with a detergent formulation containing biosurfactants produced by Pseudomonas spp. |
title_fullStr | Removal of heavy oil from contaminated surfaces with a detergent formulation containing biosurfactants produced by Pseudomonas spp. |
title_full_unstemmed | Removal of heavy oil from contaminated surfaces with a detergent formulation containing biosurfactants produced by Pseudomonas spp. |
title_short | Removal of heavy oil from contaminated surfaces with a detergent formulation containing biosurfactants produced by Pseudomonas spp. |
title_sort | removal of heavy oil from contaminated surfaces with a detergent formulation containing biosurfactants produced by pseudomonas spp. |
topic | Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8627655/ https://www.ncbi.nlm.nih.gov/pubmed/34900433 http://dx.doi.org/10.7717/peerj.12518 |
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