Cargando…

Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery

Over the past several decades, the development of engineered small particles as targeted and drug delivery systems (TDDS) has received great attention thanks to the possibility to overcome the limitations of classical cancer chemotherapy, including targeting incapability, nonspecific action and, con...

Descripción completa

Detalles Bibliográficos
Autores principales: Campora, Simona, Mohsen, Reham, Passaro, Daniel, Samir, Howida, Ashraf, Hesham, Al-Mofty, Saif El-Din, Diab, Ayman A., El-Sherbiny, Ibrahim M., Snowden, Martin J., Ghersi, Giulio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8628686/
https://www.ncbi.nlm.nih.gov/pubmed/34842694
http://dx.doi.org/10.3390/gels7040203
_version_ 1784607046702727168
author Campora, Simona
Mohsen, Reham
Passaro, Daniel
Samir, Howida
Ashraf, Hesham
Al-Mofty, Saif El-Din
Diab, Ayman A.
El-Sherbiny, Ibrahim M.
Snowden, Martin J.
Ghersi, Giulio
author_facet Campora, Simona
Mohsen, Reham
Passaro, Daniel
Samir, Howida
Ashraf, Hesham
Al-Mofty, Saif El-Din
Diab, Ayman A.
El-Sherbiny, Ibrahim M.
Snowden, Martin J.
Ghersi, Giulio
author_sort Campora, Simona
collection PubMed
description Over the past several decades, the development of engineered small particles as targeted and drug delivery systems (TDDS) has received great attention thanks to the possibility to overcome the limitations of classical cancer chemotherapy, including targeting incapability, nonspecific action and, consequently, systemic toxicity. Thus, this research aims at using a novel design of Poly(N-isopropylacrylamide) p(NIPAM)-based microgels to specifically target cancer cells and avoid the healthy ones, which is expected to decrease or eliminate the side effects of chemotherapeutic drugs. Smart NIPAM-based microgels were functionalized with acrylic acid and coupled to folic acid (FA), targeting the folate receptors overexpressed by cancer cells and to the chemotherapeutic drug doxorubicin (Dox). The successful conjugation of FA and Dox was demonstrated by dynamic light scattering (DLS), Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), UV-VIS analysis, and differential scanning calorimetry (DSC). Furthermore, viability assay performed on cancer and healthy breast cells, suggested the microgels’ biocompatibility and the cytotoxic effect of the conjugated drug. On the other hand, the specific tumor targeting of synthetized microgels was demonstrated by a co-cultured (healthy and cancer cells) assay monitored using confocal microscopy and flow cytometry. Results suggest successful targeting of cancer cells and drug release. These data support the use of pNIPAM-based microgels as good candidates as TDDS.
format Online
Article
Text
id pubmed-8628686
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86286862021-11-30 Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery Campora, Simona Mohsen, Reham Passaro, Daniel Samir, Howida Ashraf, Hesham Al-Mofty, Saif El-Din Diab, Ayman A. El-Sherbiny, Ibrahim M. Snowden, Martin J. Ghersi, Giulio Gels Article Over the past several decades, the development of engineered small particles as targeted and drug delivery systems (TDDS) has received great attention thanks to the possibility to overcome the limitations of classical cancer chemotherapy, including targeting incapability, nonspecific action and, consequently, systemic toxicity. Thus, this research aims at using a novel design of Poly(N-isopropylacrylamide) p(NIPAM)-based microgels to specifically target cancer cells and avoid the healthy ones, which is expected to decrease or eliminate the side effects of chemotherapeutic drugs. Smart NIPAM-based microgels were functionalized with acrylic acid and coupled to folic acid (FA), targeting the folate receptors overexpressed by cancer cells and to the chemotherapeutic drug doxorubicin (Dox). The successful conjugation of FA and Dox was demonstrated by dynamic light scattering (DLS), Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), UV-VIS analysis, and differential scanning calorimetry (DSC). Furthermore, viability assay performed on cancer and healthy breast cells, suggested the microgels’ biocompatibility and the cytotoxic effect of the conjugated drug. On the other hand, the specific tumor targeting of synthetized microgels was demonstrated by a co-cultured (healthy and cancer cells) assay monitored using confocal microscopy and flow cytometry. Results suggest successful targeting of cancer cells and drug release. These data support the use of pNIPAM-based microgels as good candidates as TDDS. MDPI 2021-11-08 /pmc/articles/PMC8628686/ /pubmed/34842694 http://dx.doi.org/10.3390/gels7040203 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Campora, Simona
Mohsen, Reham
Passaro, Daniel
Samir, Howida
Ashraf, Hesham
Al-Mofty, Saif El-Din
Diab, Ayman A.
El-Sherbiny, Ibrahim M.
Snowden, Martin J.
Ghersi, Giulio
Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery
title Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery
title_full Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery
title_fullStr Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery
title_full_unstemmed Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery
title_short Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery
title_sort functionalized poly(n-isopropylacrylamide)-based microgels in tumor targeting and drug delivery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8628686/
https://www.ncbi.nlm.nih.gov/pubmed/34842694
http://dx.doi.org/10.3390/gels7040203
work_keys_str_mv AT camporasimona functionalizedpolynisopropylacrylamidebasedmicrogelsintumortargetinganddrugdelivery
AT mohsenreham functionalizedpolynisopropylacrylamidebasedmicrogelsintumortargetinganddrugdelivery
AT passarodaniel functionalizedpolynisopropylacrylamidebasedmicrogelsintumortargetinganddrugdelivery
AT samirhowida functionalizedpolynisopropylacrylamidebasedmicrogelsintumortargetinganddrugdelivery
AT ashrafhesham functionalizedpolynisopropylacrylamidebasedmicrogelsintumortargetinganddrugdelivery
AT almoftysaifeldin functionalizedpolynisopropylacrylamidebasedmicrogelsintumortargetinganddrugdelivery
AT diabaymana functionalizedpolynisopropylacrylamidebasedmicrogelsintumortargetinganddrugdelivery
AT elsherbinyibrahimm functionalizedpolynisopropylacrylamidebasedmicrogelsintumortargetinganddrugdelivery
AT snowdenmartinj functionalizedpolynisopropylacrylamidebasedmicrogelsintumortargetinganddrugdelivery
AT ghersigiulio functionalizedpolynisopropylacrylamidebasedmicrogelsintumortargetinganddrugdelivery