Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage

Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegra...

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Autores principales: Bozhokin, Mikhail S., Bozhkova, Svetlana A., Rubel, Aleksandr A., Sopova, Julia V., Nashchekina, Yulia A., Bildyug, Natalya B., Khotin, Mikhail G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8628887/
https://www.ncbi.nlm.nih.gov/pubmed/34842796
http://dx.doi.org/10.3390/mps4040077
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author Bozhokin, Mikhail S.
Bozhkova, Svetlana A.
Rubel, Aleksandr A.
Sopova, Julia V.
Nashchekina, Yulia A.
Bildyug, Natalya B.
Khotin, Mikhail G.
author_facet Bozhokin, Mikhail S.
Bozhkova, Svetlana A.
Rubel, Aleksandr A.
Sopova, Julia V.
Nashchekina, Yulia A.
Bildyug, Natalya B.
Khotin, Mikhail G.
author_sort Bozhokin, Mikhail S.
collection PubMed
description Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegradable scaffold with a premixed cell culture into the damaged area of the articular surface. However, current methods for analyzing the effectiveness of such CECs have significant limitations. This study aimed to compare the SEM technique, classical histology, and cryosectioning for the analysis of CECs transplanted to hyaline cartilage.
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spelling pubmed-86288872021-11-30 Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage Bozhokin, Mikhail S. Bozhkova, Svetlana A. Rubel, Aleksandr A. Sopova, Julia V. Nashchekina, Yulia A. Bildyug, Natalya B. Khotin, Mikhail G. Methods Protoc Article Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegradable scaffold with a premixed cell culture into the damaged area of the articular surface. However, current methods for analyzing the effectiveness of such CECs have significant limitations. This study aimed to compare the SEM technique, classical histology, and cryosectioning for the analysis of CECs transplanted to hyaline cartilage. MDPI 2021-10-27 /pmc/articles/PMC8628887/ /pubmed/34842796 http://dx.doi.org/10.3390/mps4040077 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bozhokin, Mikhail S.
Bozhkova, Svetlana A.
Rubel, Aleksandr A.
Sopova, Julia V.
Nashchekina, Yulia A.
Bildyug, Natalya B.
Khotin, Mikhail G.
Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage
title Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage
title_full Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage
title_fullStr Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage
title_full_unstemmed Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage
title_short Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage
title_sort specificities of scanning electron microscopy and histological methods in assessing cell-engineered construct effectiveness for the recovery of hyaline cartilage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8628887/
https://www.ncbi.nlm.nih.gov/pubmed/34842796
http://dx.doi.org/10.3390/mps4040077
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