Cargando…

Peptide vaccines designed with the aid of immunoinformatic against Caseous Lymphadenitis promotes humoral and cellular response induction in mice

Caseous Lymphadenitis (CLA) is a chronic disease that affects also small ruminants. CLA is caused by Corynebacterium pseudotuberculosis and is responsible for high economic losses due to the formation of superficial and visceral granulomas, the latter is considered as asymptomatic CLA causing high l...

Descripción completa

Detalles Bibliográficos
Autores principales: Droppa-Almeida, Daniela, da Silva, Glenda Amaral, Gaspar, Lívia Maria do Amorim Costa, Pereyra, Beatriz Benny Sungaila, Nascimento, Roberto José Meyer, Borsuk, Sibele, Franceschi, Elton, Padilha, Francine Ferreira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629208/
https://www.ncbi.nlm.nih.gov/pubmed/34843474
http://dx.doi.org/10.1371/journal.pone.0256864
_version_ 1784607155596296192
author Droppa-Almeida, Daniela
da Silva, Glenda Amaral
Gaspar, Lívia Maria do Amorim Costa
Pereyra, Beatriz Benny Sungaila
Nascimento, Roberto José Meyer
Borsuk, Sibele
Franceschi, Elton
Padilha, Francine Ferreira
author_facet Droppa-Almeida, Daniela
da Silva, Glenda Amaral
Gaspar, Lívia Maria do Amorim Costa
Pereyra, Beatriz Benny Sungaila
Nascimento, Roberto José Meyer
Borsuk, Sibele
Franceschi, Elton
Padilha, Francine Ferreira
author_sort Droppa-Almeida, Daniela
collection PubMed
description Caseous Lymphadenitis (CLA) is a chronic disease that affects also small ruminants. CLA is caused by Corynebacterium pseudotuberculosis and is responsible for high economic losses due to the formation of superficial and visceral granulomas, the latter is considered as asymptomatic CLA causing high levels of dissemination. Several vaccination strategies, in which the use of synthetic peptides stands out. Thus, this work aimed to evaluate the protective potential of peptide vaccines designed to determine the immunodominant epitopes of CP40 against CLA in mice. The animals were divided into eight groups separated in controls (G1—PBS, G2—Saponin and G9—rCP40) and experimental (G3—pep1, G4- pep2, G5-pep3, G6-pep4, G7-pep5 and G8-pep6), these were vaccinated on days 0 and 15 by a subcutaneous route. 60 days after the first immunization, all animals were challenged with C. pseudotuberculosis. On days 0, 15, 60, and 120 after the first immunization, blood samples were taken to measure immunoglobulins. On the same day of the challenge, the splenocytes were isolated and assayed for the production of IL-2, IL-4, IL-6, IFN-γ, TNF-α, IL-17, and IL-10. After vaccinations, the animals were challenged and all of them were affected by the disease which led to their death. The G6 and G8 groups provided 10% protection and the G7 provided 20%. The G3 and G4 groups provided 30% and 40% protection respectively. The peptides showed the production of Total IgG antibodies and cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α), indicating a possible activation of the Th1 type response. However, groups G3, G5, G6, and G8 showed production of IL-17. None of the study groups showed IL-10 production. The immunogenicity of the peptides was not enough to protect these animals and it is believed that the use of adjuvants based on PAMPs may improve the immune response offered by these peptides.
format Online
Article
Text
id pubmed-8629208
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-86292082021-11-30 Peptide vaccines designed with the aid of immunoinformatic against Caseous Lymphadenitis promotes humoral and cellular response induction in mice Droppa-Almeida, Daniela da Silva, Glenda Amaral Gaspar, Lívia Maria do Amorim Costa Pereyra, Beatriz Benny Sungaila Nascimento, Roberto José Meyer Borsuk, Sibele Franceschi, Elton Padilha, Francine Ferreira PLoS One Research Article Caseous Lymphadenitis (CLA) is a chronic disease that affects also small ruminants. CLA is caused by Corynebacterium pseudotuberculosis and is responsible for high economic losses due to the formation of superficial and visceral granulomas, the latter is considered as asymptomatic CLA causing high levels of dissemination. Several vaccination strategies, in which the use of synthetic peptides stands out. Thus, this work aimed to evaluate the protective potential of peptide vaccines designed to determine the immunodominant epitopes of CP40 against CLA in mice. The animals were divided into eight groups separated in controls (G1—PBS, G2—Saponin and G9—rCP40) and experimental (G3—pep1, G4- pep2, G5-pep3, G6-pep4, G7-pep5 and G8-pep6), these were vaccinated on days 0 and 15 by a subcutaneous route. 60 days after the first immunization, all animals were challenged with C. pseudotuberculosis. On days 0, 15, 60, and 120 after the first immunization, blood samples were taken to measure immunoglobulins. On the same day of the challenge, the splenocytes were isolated and assayed for the production of IL-2, IL-4, IL-6, IFN-γ, TNF-α, IL-17, and IL-10. After vaccinations, the animals were challenged and all of them were affected by the disease which led to their death. The G6 and G8 groups provided 10% protection and the G7 provided 20%. The G3 and G4 groups provided 30% and 40% protection respectively. The peptides showed the production of Total IgG antibodies and cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α), indicating a possible activation of the Th1 type response. However, groups G3, G5, G6, and G8 showed production of IL-17. None of the study groups showed IL-10 production. The immunogenicity of the peptides was not enough to protect these animals and it is believed that the use of adjuvants based on PAMPs may improve the immune response offered by these peptides. Public Library of Science 2021-11-29 /pmc/articles/PMC8629208/ /pubmed/34843474 http://dx.doi.org/10.1371/journal.pone.0256864 Text en © 2021 Droppa-Almeida et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Droppa-Almeida, Daniela
da Silva, Glenda Amaral
Gaspar, Lívia Maria do Amorim Costa
Pereyra, Beatriz Benny Sungaila
Nascimento, Roberto José Meyer
Borsuk, Sibele
Franceschi, Elton
Padilha, Francine Ferreira
Peptide vaccines designed with the aid of immunoinformatic against Caseous Lymphadenitis promotes humoral and cellular response induction in mice
title Peptide vaccines designed with the aid of immunoinformatic against Caseous Lymphadenitis promotes humoral and cellular response induction in mice
title_full Peptide vaccines designed with the aid of immunoinformatic against Caseous Lymphadenitis promotes humoral and cellular response induction in mice
title_fullStr Peptide vaccines designed with the aid of immunoinformatic against Caseous Lymphadenitis promotes humoral and cellular response induction in mice
title_full_unstemmed Peptide vaccines designed with the aid of immunoinformatic against Caseous Lymphadenitis promotes humoral and cellular response induction in mice
title_short Peptide vaccines designed with the aid of immunoinformatic against Caseous Lymphadenitis promotes humoral and cellular response induction in mice
title_sort peptide vaccines designed with the aid of immunoinformatic against caseous lymphadenitis promotes humoral and cellular response induction in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629208/
https://www.ncbi.nlm.nih.gov/pubmed/34843474
http://dx.doi.org/10.1371/journal.pone.0256864
work_keys_str_mv AT droppaalmeidadaniela peptidevaccinesdesignedwiththeaidofimmunoinformaticagainstcaseouslymphadenitispromoteshumoralandcellularresponseinductioninmice
AT dasilvaglendaamaral peptidevaccinesdesignedwiththeaidofimmunoinformaticagainstcaseouslymphadenitispromoteshumoralandcellularresponseinductioninmice
AT gasparliviamariadoamorimcosta peptidevaccinesdesignedwiththeaidofimmunoinformaticagainstcaseouslymphadenitispromoteshumoralandcellularresponseinductioninmice
AT pereyrabeatrizbennysungaila peptidevaccinesdesignedwiththeaidofimmunoinformaticagainstcaseouslymphadenitispromoteshumoralandcellularresponseinductioninmice
AT nascimentorobertojosemeyer peptidevaccinesdesignedwiththeaidofimmunoinformaticagainstcaseouslymphadenitispromoteshumoralandcellularresponseinductioninmice
AT borsuksibele peptidevaccinesdesignedwiththeaidofimmunoinformaticagainstcaseouslymphadenitispromoteshumoralandcellularresponseinductioninmice
AT franceschielton peptidevaccinesdesignedwiththeaidofimmunoinformaticagainstcaseouslymphadenitispromoteshumoralandcellularresponseinductioninmice
AT padilhafrancineferreira peptidevaccinesdesignedwiththeaidofimmunoinformaticagainstcaseouslymphadenitispromoteshumoralandcellularresponseinductioninmice