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Infection, recovery and re-infection of farmed mink with SARS-CoV-2

Mink, on a farm with about 15,000 animals, became infected with SARS-CoV-2. Over 75% of tested animals were positive for SARS-CoV-2 RNA in throat swabs and 100% of tested animals were seropositive. The virus responsible had a deletion of nucleotides encoding residues H69 and V70 within the spike pro...

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Autores principales: Rasmussen, Thomas Bruun, Fonager, Jannik, Jørgensen, Charlotte Sværke, Lassaunière, Ria, Hammer, Anne Sofie, Quaade, Michelle Lauge, Boklund, Anette, Lohse, Louise, Strandbygaard, Bertel, Rasmussen, Morten, Michaelsen, Thomas Yssing, Mortensen, Sten, Fomsgaard, Anders, Belsham, Graham J., Bøtner, Anette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629378/
https://www.ncbi.nlm.nih.gov/pubmed/34780574
http://dx.doi.org/10.1371/journal.ppat.1010068
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author Rasmussen, Thomas Bruun
Fonager, Jannik
Jørgensen, Charlotte Sværke
Lassaunière, Ria
Hammer, Anne Sofie
Quaade, Michelle Lauge
Boklund, Anette
Lohse, Louise
Strandbygaard, Bertel
Rasmussen, Morten
Michaelsen, Thomas Yssing
Mortensen, Sten
Fomsgaard, Anders
Belsham, Graham J.
Bøtner, Anette
author_facet Rasmussen, Thomas Bruun
Fonager, Jannik
Jørgensen, Charlotte Sværke
Lassaunière, Ria
Hammer, Anne Sofie
Quaade, Michelle Lauge
Boklund, Anette
Lohse, Louise
Strandbygaard, Bertel
Rasmussen, Morten
Michaelsen, Thomas Yssing
Mortensen, Sten
Fomsgaard, Anders
Belsham, Graham J.
Bøtner, Anette
author_sort Rasmussen, Thomas Bruun
collection PubMed
description Mink, on a farm with about 15,000 animals, became infected with SARS-CoV-2. Over 75% of tested animals were positive for SARS-CoV-2 RNA in throat swabs and 100% of tested animals were seropositive. The virus responsible had a deletion of nucleotides encoding residues H69 and V70 within the spike protein gene as well as the A22920T mutation, resulting in the Y453F substitution within this protein, seen previously in mink. The infected mink recovered and after free-testing of 300 mink (a level giving 93% confidence of detecting a 1% prevalence), the animals remained seropositive. During further follow-up studies, after a period of more than 2 months without any virus detection, over 75% of tested animals again scored positive for SARS-CoV-2 RNA. Whole genome sequencing showed that the viruses circulating during this re-infection were most closely related to those identified in the first outbreak on this farm but additional sequence changes had occurred. Animals had much higher levels of anti-SARS-CoV-2 antibodies in serum samples after the second round of infection than at free-testing or during recovery from initial infection, consistent with a boosted immune response. Thus, it was concluded that following recovery from an initial infection, seropositive mink were readily re-infected by SARS-CoV-2.
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spelling pubmed-86293782021-11-30 Infection, recovery and re-infection of farmed mink with SARS-CoV-2 Rasmussen, Thomas Bruun Fonager, Jannik Jørgensen, Charlotte Sværke Lassaunière, Ria Hammer, Anne Sofie Quaade, Michelle Lauge Boklund, Anette Lohse, Louise Strandbygaard, Bertel Rasmussen, Morten Michaelsen, Thomas Yssing Mortensen, Sten Fomsgaard, Anders Belsham, Graham J. Bøtner, Anette PLoS Pathog Research Article Mink, on a farm with about 15,000 animals, became infected with SARS-CoV-2. Over 75% of tested animals were positive for SARS-CoV-2 RNA in throat swabs and 100% of tested animals were seropositive. The virus responsible had a deletion of nucleotides encoding residues H69 and V70 within the spike protein gene as well as the A22920T mutation, resulting in the Y453F substitution within this protein, seen previously in mink. The infected mink recovered and after free-testing of 300 mink (a level giving 93% confidence of detecting a 1% prevalence), the animals remained seropositive. During further follow-up studies, after a period of more than 2 months without any virus detection, over 75% of tested animals again scored positive for SARS-CoV-2 RNA. Whole genome sequencing showed that the viruses circulating during this re-infection were most closely related to those identified in the first outbreak on this farm but additional sequence changes had occurred. Animals had much higher levels of anti-SARS-CoV-2 antibodies in serum samples after the second round of infection than at free-testing or during recovery from initial infection, consistent with a boosted immune response. Thus, it was concluded that following recovery from an initial infection, seropositive mink were readily re-infected by SARS-CoV-2. Public Library of Science 2021-11-15 /pmc/articles/PMC8629378/ /pubmed/34780574 http://dx.doi.org/10.1371/journal.ppat.1010068 Text en © 2021 Rasmussen et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rasmussen, Thomas Bruun
Fonager, Jannik
Jørgensen, Charlotte Sværke
Lassaunière, Ria
Hammer, Anne Sofie
Quaade, Michelle Lauge
Boklund, Anette
Lohse, Louise
Strandbygaard, Bertel
Rasmussen, Morten
Michaelsen, Thomas Yssing
Mortensen, Sten
Fomsgaard, Anders
Belsham, Graham J.
Bøtner, Anette
Infection, recovery and re-infection of farmed mink with SARS-CoV-2
title Infection, recovery and re-infection of farmed mink with SARS-CoV-2
title_full Infection, recovery and re-infection of farmed mink with SARS-CoV-2
title_fullStr Infection, recovery and re-infection of farmed mink with SARS-CoV-2
title_full_unstemmed Infection, recovery and re-infection of farmed mink with SARS-CoV-2
title_short Infection, recovery and re-infection of farmed mink with SARS-CoV-2
title_sort infection, recovery and re-infection of farmed mink with sars-cov-2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629378/
https://www.ncbi.nlm.nih.gov/pubmed/34780574
http://dx.doi.org/10.1371/journal.ppat.1010068
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