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Gut microbiota in forty cases of egyptian relapsing remitting multiple sclerosis

BACKGROUND AND OBJECTIVES: Gut microbiota is assumed to play an essential role in the pathogenesis of multiple sclerosis (MS). This study aimed to investigate the abundance of some gut microbiota among Egyptian patients with relapsing remitting multiple sclerosis (RR-MS). MATERIALS AND METHODS: Fort...

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Autores principales: Elgendy, Sherein G., Abd-Elhameed, Rawan, Daef, Enas, Mohammed, Shereen M., Hassan, Hebatallah M., El-Mokhtar, Mohamed A., Nasreldein, Ahmed, Khedr, Eman M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629825/
https://www.ncbi.nlm.nih.gov/pubmed/34900161
http://dx.doi.org/10.18502/ijm.v13i5.7428
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author Elgendy, Sherein G.
Abd-Elhameed, Rawan
Daef, Enas
Mohammed, Shereen M.
Hassan, Hebatallah M.
El-Mokhtar, Mohamed A.
Nasreldein, Ahmed
Khedr, Eman M.
author_facet Elgendy, Sherein G.
Abd-Elhameed, Rawan
Daef, Enas
Mohammed, Shereen M.
Hassan, Hebatallah M.
El-Mokhtar, Mohamed A.
Nasreldein, Ahmed
Khedr, Eman M.
author_sort Elgendy, Sherein G.
collection PubMed
description BACKGROUND AND OBJECTIVES: Gut microbiota is assumed to play an essential role in the pathogenesis of multiple sclerosis (MS). This study aimed to investigate the abundance of some gut microbiota among Egyptian patients with relapsing remitting multiple sclerosis (RR-MS). MATERIALS AND METHODS: Forty cases of RR-MS diagnosed according to McDonald diagnostic criteria (2017) were recruited consecutively from the Department of Neurology, Assiut University Hospitals. The results were compared with 22 healthy age and sex matched control subjects. DNA was extracted from stool and measures made of concentration and copy number of bacterial organisms by real-time PCR using group specific primers for 16S rRNA targeting predominant genera of gut microbiota previously hypothesized to participate in MS pathogenesis. RESULTS: The mean age was 31.4 ± 8.8 yrs; 75% of the patients were women. The mean and SD of EDSS score was 3.43 ± 1.35. Seven cases had cervical cord plaques (17%). There were significantly increased copy numbers of Desulfovibrio, Actinobacteria, Firmcutes, and Lactic acid bacteria in patients compared with the control group. In contrast there was a significantly lower level of Clostridium cluster IV in the patients. Patients who had EDSS < 3.5 had a significantly higher copy number of Actinobacteria, Bacteroidetes, and Bifidobacterium, compared with patients who had EDSS > 3.5. There was a significant negative correlation between duration of illness and copy number of Firmcutes, Akkermansia, and Lactic acid bacteria (P = 0.01, 0.04, and 0.004 respectively). CONCLUSION: The changes in gut microbiota are associated with exacerbation of MS disease. Disruption of the intestinal microbiota results in the depletion or enrichment of certain bacteria that may affect the immune balance leading to predisposition to MS.
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spelling pubmed-86298252021-12-10 Gut microbiota in forty cases of egyptian relapsing remitting multiple sclerosis Elgendy, Sherein G. Abd-Elhameed, Rawan Daef, Enas Mohammed, Shereen M. Hassan, Hebatallah M. El-Mokhtar, Mohamed A. Nasreldein, Ahmed Khedr, Eman M. Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Gut microbiota is assumed to play an essential role in the pathogenesis of multiple sclerosis (MS). This study aimed to investigate the abundance of some gut microbiota among Egyptian patients with relapsing remitting multiple sclerosis (RR-MS). MATERIALS AND METHODS: Forty cases of RR-MS diagnosed according to McDonald diagnostic criteria (2017) were recruited consecutively from the Department of Neurology, Assiut University Hospitals. The results were compared with 22 healthy age and sex matched control subjects. DNA was extracted from stool and measures made of concentration and copy number of bacterial organisms by real-time PCR using group specific primers for 16S rRNA targeting predominant genera of gut microbiota previously hypothesized to participate in MS pathogenesis. RESULTS: The mean age was 31.4 ± 8.8 yrs; 75% of the patients were women. The mean and SD of EDSS score was 3.43 ± 1.35. Seven cases had cervical cord plaques (17%). There were significantly increased copy numbers of Desulfovibrio, Actinobacteria, Firmcutes, and Lactic acid bacteria in patients compared with the control group. In contrast there was a significantly lower level of Clostridium cluster IV in the patients. Patients who had EDSS < 3.5 had a significantly higher copy number of Actinobacteria, Bacteroidetes, and Bifidobacterium, compared with patients who had EDSS > 3.5. There was a significant negative correlation between duration of illness and copy number of Firmcutes, Akkermansia, and Lactic acid bacteria (P = 0.01, 0.04, and 0.004 respectively). CONCLUSION: The changes in gut microbiota are associated with exacerbation of MS disease. Disruption of the intestinal microbiota results in the depletion or enrichment of certain bacteria that may affect the immune balance leading to predisposition to MS. Tehran University of Medical Sciences 2021-10 /pmc/articles/PMC8629825/ /pubmed/34900161 http://dx.doi.org/10.18502/ijm.v13i5.7428 Text en Copyright © 2021 The Authors. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Elgendy, Sherein G.
Abd-Elhameed, Rawan
Daef, Enas
Mohammed, Shereen M.
Hassan, Hebatallah M.
El-Mokhtar, Mohamed A.
Nasreldein, Ahmed
Khedr, Eman M.
Gut microbiota in forty cases of egyptian relapsing remitting multiple sclerosis
title Gut microbiota in forty cases of egyptian relapsing remitting multiple sclerosis
title_full Gut microbiota in forty cases of egyptian relapsing remitting multiple sclerosis
title_fullStr Gut microbiota in forty cases of egyptian relapsing remitting multiple sclerosis
title_full_unstemmed Gut microbiota in forty cases of egyptian relapsing remitting multiple sclerosis
title_short Gut microbiota in forty cases of egyptian relapsing remitting multiple sclerosis
title_sort gut microbiota in forty cases of egyptian relapsing remitting multiple sclerosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629825/
https://www.ncbi.nlm.nih.gov/pubmed/34900161
http://dx.doi.org/10.18502/ijm.v13i5.7428
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