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The human intervertebral disc as a source of DNA for molecular identification

Genetic analyses such as STR-typing are routinely used for identification purposes in forensic casework. Although genotyping techniques only require a minimum amount of DNA to provide a genetic profile, DNA quality differs not only between but also within tissues during ongoing decomposition. Initia...

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Autores principales: Becker, Julia, Mahlke, Nina Sophia, Ritz-Timme, Stefanie, Boehme, Petra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629888/
https://www.ncbi.nlm.nih.gov/pubmed/34292481
http://dx.doi.org/10.1007/s12024-021-00401-0
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author Becker, Julia
Mahlke, Nina Sophia
Ritz-Timme, Stefanie
Boehme, Petra
author_facet Becker, Julia
Mahlke, Nina Sophia
Ritz-Timme, Stefanie
Boehme, Petra
author_sort Becker, Julia
collection PubMed
description Genetic analyses such as STR-typing are routinely used for identification purposes in forensic casework. Although genotyping techniques only require a minimum amount of DNA to provide a genetic profile, DNA quality differs not only between but also within tissues during ongoing decomposition. Initiated by a recent case where, due to the constitution of the body, preferred tissue was not available or only resulted in a partial and not usable DNA profile, the analysis of intervertebral discs as a source of DNA was considered. As the analysis of this tissue resulted in a high quality DNA profile a further study was performed in which thirty intervertebral discs dissected from bodies in different stages of decay were analyzed. All samples yielded good quality DNA in quantities suitable for STR-based amplification with no or only low degradation indices, resulting in complete genetic profiles. These results demonstrate the robustness of human intervertebral disc tissue as a source of DNA for molecular identification purposes.
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spelling pubmed-86298882021-12-15 The human intervertebral disc as a source of DNA for molecular identification Becker, Julia Mahlke, Nina Sophia Ritz-Timme, Stefanie Boehme, Petra Forensic Sci Med Pathol Technical Report Genetic analyses such as STR-typing are routinely used for identification purposes in forensic casework. Although genotyping techniques only require a minimum amount of DNA to provide a genetic profile, DNA quality differs not only between but also within tissues during ongoing decomposition. Initiated by a recent case where, due to the constitution of the body, preferred tissue was not available or only resulted in a partial and not usable DNA profile, the analysis of intervertebral discs as a source of DNA was considered. As the analysis of this tissue resulted in a high quality DNA profile a further study was performed in which thirty intervertebral discs dissected from bodies in different stages of decay were analyzed. All samples yielded good quality DNA in quantities suitable for STR-based amplification with no or only low degradation indices, resulting in complete genetic profiles. These results demonstrate the robustness of human intervertebral disc tissue as a source of DNA for molecular identification purposes. Springer US 2021-07-22 2021 /pmc/articles/PMC8629888/ /pubmed/34292481 http://dx.doi.org/10.1007/s12024-021-00401-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Technical Report
Becker, Julia
Mahlke, Nina Sophia
Ritz-Timme, Stefanie
Boehme, Petra
The human intervertebral disc as a source of DNA for molecular identification
title The human intervertebral disc as a source of DNA for molecular identification
title_full The human intervertebral disc as a source of DNA for molecular identification
title_fullStr The human intervertebral disc as a source of DNA for molecular identification
title_full_unstemmed The human intervertebral disc as a source of DNA for molecular identification
title_short The human intervertebral disc as a source of DNA for molecular identification
title_sort human intervertebral disc as a source of dna for molecular identification
topic Technical Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629888/
https://www.ncbi.nlm.nih.gov/pubmed/34292481
http://dx.doi.org/10.1007/s12024-021-00401-0
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