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The human intervertebral disc as a source of DNA for molecular identification
Genetic analyses such as STR-typing are routinely used for identification purposes in forensic casework. Although genotyping techniques only require a minimum amount of DNA to provide a genetic profile, DNA quality differs not only between but also within tissues during ongoing decomposition. Initia...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629888/ https://www.ncbi.nlm.nih.gov/pubmed/34292481 http://dx.doi.org/10.1007/s12024-021-00401-0 |
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author | Becker, Julia Mahlke, Nina Sophia Ritz-Timme, Stefanie Boehme, Petra |
author_facet | Becker, Julia Mahlke, Nina Sophia Ritz-Timme, Stefanie Boehme, Petra |
author_sort | Becker, Julia |
collection | PubMed |
description | Genetic analyses such as STR-typing are routinely used for identification purposes in forensic casework. Although genotyping techniques only require a minimum amount of DNA to provide a genetic profile, DNA quality differs not only between but also within tissues during ongoing decomposition. Initiated by a recent case where, due to the constitution of the body, preferred tissue was not available or only resulted in a partial and not usable DNA profile, the analysis of intervertebral discs as a source of DNA was considered. As the analysis of this tissue resulted in a high quality DNA profile a further study was performed in which thirty intervertebral discs dissected from bodies in different stages of decay were analyzed. All samples yielded good quality DNA in quantities suitable for STR-based amplification with no or only low degradation indices, resulting in complete genetic profiles. These results demonstrate the robustness of human intervertebral disc tissue as a source of DNA for molecular identification purposes. |
format | Online Article Text |
id | pubmed-8629888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-86298882021-12-15 The human intervertebral disc as a source of DNA for molecular identification Becker, Julia Mahlke, Nina Sophia Ritz-Timme, Stefanie Boehme, Petra Forensic Sci Med Pathol Technical Report Genetic analyses such as STR-typing are routinely used for identification purposes in forensic casework. Although genotyping techniques only require a minimum amount of DNA to provide a genetic profile, DNA quality differs not only between but also within tissues during ongoing decomposition. Initiated by a recent case where, due to the constitution of the body, preferred tissue was not available or only resulted in a partial and not usable DNA profile, the analysis of intervertebral discs as a source of DNA was considered. As the analysis of this tissue resulted in a high quality DNA profile a further study was performed in which thirty intervertebral discs dissected from bodies in different stages of decay were analyzed. All samples yielded good quality DNA in quantities suitable for STR-based amplification with no or only low degradation indices, resulting in complete genetic profiles. These results demonstrate the robustness of human intervertebral disc tissue as a source of DNA for molecular identification purposes. Springer US 2021-07-22 2021 /pmc/articles/PMC8629888/ /pubmed/34292481 http://dx.doi.org/10.1007/s12024-021-00401-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Technical Report Becker, Julia Mahlke, Nina Sophia Ritz-Timme, Stefanie Boehme, Petra The human intervertebral disc as a source of DNA for molecular identification |
title | The human intervertebral disc as a source of DNA for molecular identification |
title_full | The human intervertebral disc as a source of DNA for molecular identification |
title_fullStr | The human intervertebral disc as a source of DNA for molecular identification |
title_full_unstemmed | The human intervertebral disc as a source of DNA for molecular identification |
title_short | The human intervertebral disc as a source of DNA for molecular identification |
title_sort | human intervertebral disc as a source of dna for molecular identification |
topic | Technical Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629888/ https://www.ncbi.nlm.nih.gov/pubmed/34292481 http://dx.doi.org/10.1007/s12024-021-00401-0 |
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