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In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications

Natural mycelial biomass (MB) and exopolysaccharides (EPS) of Malaysian tiger milk mushroom Lignosus rhinocerus are considered high-end components due to their high commercial potential value in drug discovery. This study aims to evaluate the toxicity of the mushroom extracts’ generated in a bioreac...

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Autores principales: Usuldin, Siti Rokhiyah Ahmad, Wan-Mohtar, Wan Abd Al Qadr Imad, Ilham, Zul, Jamaludin, Adi Ainurzaman, Abdullah, Nur Raihan, Rowan, Neil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629991/
https://www.ncbi.nlm.nih.gov/pubmed/34845290
http://dx.doi.org/10.1038/s41598-021-02486-7
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author Usuldin, Siti Rokhiyah Ahmad
Wan-Mohtar, Wan Abd Al Qadr Imad
Ilham, Zul
Jamaludin, Adi Ainurzaman
Abdullah, Nur Raihan
Rowan, Neil
author_facet Usuldin, Siti Rokhiyah Ahmad
Wan-Mohtar, Wan Abd Al Qadr Imad
Ilham, Zul
Jamaludin, Adi Ainurzaman
Abdullah, Nur Raihan
Rowan, Neil
author_sort Usuldin, Siti Rokhiyah Ahmad
collection PubMed
description Natural mycelial biomass (MB) and exopolysaccharides (EPS) of Malaysian tiger milk mushroom Lignosus rhinocerus are considered high-end components due to their high commercial potential value in drug discovery. This study aims to evaluate the toxicity of the mushroom extracts’ generated in a bioreactor using the zebrafish embryo toxicity (ZFET) model assay as a new therapy for treating asthma. Both MB and EPS extracts, at concentrations 0.16–10 mg/mL, were tested for ZFET and early development effects on Zebrafish Embryos (ZE) during 24–120 h post-fertilisation (HPF). Findings revealed that MB was deemed safe with an LC(50) of 0.77 mg/mL; the EPS were non-toxic (LC(50) of 0.41 mg/mL). Neither MB nor EPS delayed hatching nor teratogenic defects in the treated ZE at a 2.5 mg/mL dose. There were no significant changes in the ZE heart rate after treatments with MB (130 beats/min) and EPS (140 beats/min), compared to that of normal ZE (120–180 beats/min). Mixing both natural compounds MB and EPS did not affect toxicity using ZFET testing; thus, intimating their safe future use as therapeutic interventions. This represents the first study to have used the ZFET assay on MB and EPS extracts of L. rhinocerus for future health applications.
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spelling pubmed-86299912021-12-01 In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications Usuldin, Siti Rokhiyah Ahmad Wan-Mohtar, Wan Abd Al Qadr Imad Ilham, Zul Jamaludin, Adi Ainurzaman Abdullah, Nur Raihan Rowan, Neil Sci Rep Article Natural mycelial biomass (MB) and exopolysaccharides (EPS) of Malaysian tiger milk mushroom Lignosus rhinocerus are considered high-end components due to their high commercial potential value in drug discovery. This study aims to evaluate the toxicity of the mushroom extracts’ generated in a bioreactor using the zebrafish embryo toxicity (ZFET) model assay as a new therapy for treating asthma. Both MB and EPS extracts, at concentrations 0.16–10 mg/mL, were tested for ZFET and early development effects on Zebrafish Embryos (ZE) during 24–120 h post-fertilisation (HPF). Findings revealed that MB was deemed safe with an LC(50) of 0.77 mg/mL; the EPS were non-toxic (LC(50) of 0.41 mg/mL). Neither MB nor EPS delayed hatching nor teratogenic defects in the treated ZE at a 2.5 mg/mL dose. There were no significant changes in the ZE heart rate after treatments with MB (130 beats/min) and EPS (140 beats/min), compared to that of normal ZE (120–180 beats/min). Mixing both natural compounds MB and EPS did not affect toxicity using ZFET testing; thus, intimating their safe future use as therapeutic interventions. This represents the first study to have used the ZFET assay on MB and EPS extracts of L. rhinocerus for future health applications. Nature Publishing Group UK 2021-11-29 /pmc/articles/PMC8629991/ /pubmed/34845290 http://dx.doi.org/10.1038/s41598-021-02486-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Usuldin, Siti Rokhiyah Ahmad
Wan-Mohtar, Wan Abd Al Qadr Imad
Ilham, Zul
Jamaludin, Adi Ainurzaman
Abdullah, Nur Raihan
Rowan, Neil
In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications
title In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications
title_full In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications
title_fullStr In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications
title_full_unstemmed In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications
title_short In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications
title_sort in vivo toxicity of bioreactor-grown biomass and exopolysaccharides from malaysian tiger milk mushroom mycelium for potential future health applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629991/
https://www.ncbi.nlm.nih.gov/pubmed/34845290
http://dx.doi.org/10.1038/s41598-021-02486-7
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