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A OneStep Solution to Fix and Stain Cells for Correlative Live and Fixed Microscopy
Correlating the location of subcellular structures with dynamic cellular behaviors is difficult when working with organisms that lack the molecular genetic tools needed for expressing fluorescent protein fusions. Here, we describe a protocol for fixing, permeabilizing, and staining cells in a single...
Autores principales: | Velle, Katrina B., Fermino do Rosário, Carline, Wadsworth, Patricia, Fritz‐Laylin, Lillian K. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8630750/ https://www.ncbi.nlm.nih.gov/pubmed/34826344 http://dx.doi.org/10.1002/cpz1.308 |
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