Cargando…

Immobilization of Recombinant Fluorescent Biosensors Permits Imaging of Extracellular Ion Signals

[Image: see text] Given the importance of ion gradients and fluxes in biology, monitoring ions locally at the exterior of the plasma membrane of intact cells in a noninvasive manner is highly desirable but challenging. Classical targeting of genetically encoded biosensors at the exterior of cell sur...

Descripción completa

Detalles Bibliográficos
Autores principales:	Burgstaller, Sandra, Bischof, Helmut, Rauter, Thomas, Schmidt, Tony, Schindl, Rainer, Patz, Silke, Groschup, Bernhard, Filser, Severin, van den Boom, Lucas, Sasse, Philipp, Lukowski, Robert, Plesnila, Nikolaus, Graier, Wolfgang F., Malli, Roland
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2021
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8630794/ https://www.ncbi.nlm.nih.gov/pubmed/34752056 http://dx.doi.org/10.1021/acssensors.1c01369

Ejemplares similares

Potassium ions promote hexokinase-II dependent glycolysis
por: Bischof, Helmut, et al.
Publicado: (2021)

Potassium ions promote hexokinase-II dependent glycolysis
por: Bischof, Helmut, et al.
Publicado: (2021)

Unveiling the K(+)-sensitivity of cell metabolism using genetically encoded, FRET-based K(+), glucose, and ATP biosensors
por: Bischof, Helmut, et al.
Publicado: (2021)

Investigating the K(+) sensitivity of cellular metabolism by extracellular flux analysis
por: Burgstaller, Sandra, et al.
Publicado: (2021)

Live-Cell Imaging of Physiologically Relevant Metal Ions Using Genetically Encoded FRET-Based Probes
por: Bischof, Helmut, et al.
Publicado: (2019)

ER-to-Golgi Transport in HeLa Cells Displays High Resilience to Ca(2+) and Energy Stresses
por: Rauter, Thomas, et al.
Publicado: (2020)

Monitoring extracellular ion and metabolite dynamics with recombinant nanobody-fused biosensors
por: Burgstaller, Sandra, et al.
Publicado: (2022)

Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes
por: Rauter, Thomas, et al.
Publicado: (2020)

Development and Application of Sub-Mitochondrial Targeted Ca(2 +) Biosensors
por: Waldeck-Weiermair, Markus, et al.
Publicado: (2019)

Visualization of Sirtuin 4 Distribution between Mitochondria and the Nucleus, Based on Bimolecular Fluorescence Self-Complementation
por: Ramadani-Muja, Jeta, et al.
Publicado: (2019)

Light Stimulation of Neurons on Organic Photocapacitors Induces Action Potentials with Millisecond Precision
por: Schmidt, Tony, et al.
Publicado: (2022)

Genetic biosensors for imaging nitric oxide in single cells
por: Eroglu, Emrah, et al.
Publicado: (2018)

Purification and Application of Genetically Encoded Potassium Ion Indicators for Quantification of Potassium Ion Concentrations within Biological Samples
por: Bischof, H., et al.
Publicado: (2019)

Tracking intra‐ and inter‐organelle signaling of mitochondria
por: Madreiter‐Sokolowski, Corina T., et al.
Publicado: (2019)

BK channels sustain neuronal Ca(2+) oscillations to support hippocampal long-term potentiation and memory formation
por: Pham, Thomas, et al.
Publicado: (2023)

Mitochondria supply ATP to the ER through a mechanism antagonized by cytosolic Ca(2+)
por: Yong, Jing, et al.
Publicado: (2019)

Probing Subcellular Iron Availability with Genetically Encoded Nitric Oxide Biosensors
por: Sevimli, Gulsah, et al.
Publicado: (2022)

Immobilized Enzymes in Biosensor Applications
por: Nguyen, Hoang Hiep, et al.
Publicado: (2019)

pH-Lemon, a Fluorescent Protein-Based pH Reporter for Acidic Compartments
por: Burgstaller, Sandra, et al.
Publicado: (2019)

Newer Point-of-Care Biosensors Are Expected to Permit Early Detection and Better Management of Acute and CKD
por: Cerdá, Jorge
Publicado: (2018)

Real-time visualization of distinct nitric oxide generation of nitric oxide synthase isoforms in single cells
por: Eroglu, Emrah, et al.
Publicado: (2017)

Novel genetically encoded fluorescent probes enable real-time detection of potassium in vitro and in vivo
por: Bischof, Helmut, et al.
Publicado: (2017)

IK(Ca) channels control breast cancer metabolism including AMPK-driven autophagy
por: Gross, Dominic, et al.
Publicado: (2022)

Generation of Red-Shifted Cameleons for Imaging Ca(2+) Dynamics of the Endoplasmic Reticulum
por: Waldeck-Weiermair, Markus, et al.
Publicado: (2015)

Enzyme Immobilization on Gold Nanoparticles for Electrochemical Glucose Biosensors
por: Lipińska, Wiktoria, et al.
Publicado: (2021)

Substrate Materials for Biomolecular Immobilization within Electrochemical Biosensors
por: Suni, Ian Ivar
Publicado: (2021)

Microfluidic Electrochemical Glucose Biosensor with In Situ Enzyme Immobilization
por: Lokar, Nina, et al.
Publicado: (2023)

Pathophysiological Role of Global Cerebral Ischemia following Subarachnoid Hemorrhage: The Current Experimental Evidence
por: Plesnila, Nikolaus
Publicado: (2013)

Electrocatalytic sulfite biosensor with human sulfite oxidase co-immobilized with cytochrome c in a polyelectrolyte-containing multilayer
por: Spricigo, Roberto, et al.
Publicado: (2008)

Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States
por: Marchetti, Marialaura, et al.
Publicado: (2019)

The Effects of Three-Dimensional Ligand Immobilization on Kinetic Measurements in Biosensors
por: Chiodi, Elisa, et al.
Publicado: (2022)

Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review
por: Bounegru, Alexandra Virginia, et al.
Publicado: (2023)

Biopolymers Used for Receptor Immobilization for Nickel-Detection Biosensors in Food
por: Anchidin-Norocel, Liliana, et al.
Publicado: (2023)

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring
por: Sun, Ganchao, et al.
Publicado: (2023)

Tradeable permits : policy evaluation, design and reform.
Publicado: (2004)

Real-Time Imaging of Mitochondrial ATP Dynamics Reveals the Metabolic Setting of Single Cells
por: Depaoli, Maria R., et al.
Publicado: (2018)

Immobilization Techniques in the Fabrication of Nanomaterial-Based Electrochemical Biosensors: A Review
por: Putzbach, William, et al.
Publicado: (2013)

Contributions of the immune system to the pathophysiology of traumatic brain injury – evidence by intravital microscopy
por: Schwarzmaier, Susanne M., et al.
Publicado: (2014)

The GPR55 agonist lysophosphatidylinositol directly activates intermediate-conductance Ca(2+)-activated K(+) channels
por: Bondarenko, Alexander I., et al.
Publicado: (2011)

The GPR55 agonist lysophosphatidylinositol acts as an intracellular messenger and bidirectionally modulates Ca(2+)-activated large-conductance K(+) channels in endothelial cells
por: Bondarenko, Alexander I., et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_aip3ri9fr463mhdmljtds7l9qo