Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2

We compared plasma and dried blood spots for detection of SARS-CoV-2 IgG antibodies. There was a good correlation between IgG values measured by both sampling methods, r = 0.935 and 0.965 for Receptor Binding Domain and full-length spike protein of SARS-CoV-2. Bland-Altman assessment showed good agr...

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Autores principales: Kwatra, Gaurav, Nunes, Marta, Dhar, Nisha, Baillie, Vicky, Serafin, Natali, Jones, Stephanie, Madhi, Shabir A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631068/
https://www.ncbi.nlm.nih.gov/pubmed/34861318
http://dx.doi.org/10.1016/j.jviromet.2021.114394
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author Kwatra, Gaurav
Nunes, Marta
Dhar, Nisha
Baillie, Vicky
Serafin, Natali
Jones, Stephanie
Madhi, Shabir A.
author_facet Kwatra, Gaurav
Nunes, Marta
Dhar, Nisha
Baillie, Vicky
Serafin, Natali
Jones, Stephanie
Madhi, Shabir A.
author_sort Kwatra, Gaurav
collection PubMed
description We compared plasma and dried blood spots for detection of SARS-CoV-2 IgG antibodies. There was a good correlation between IgG values measured by both sampling methods, r = 0.935 and 0.965 for Receptor Binding Domain and full-length spike protein of SARS-CoV-2. Bland-Altman assessment showed good agreement between two sampling methods. Dried blood spots is a more pragmatic method for collecting samples for sero-epidemiological surveys of SARS-CoV-2 infection.
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spelling pubmed-86310682021-11-30 Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2 Kwatra, Gaurav Nunes, Marta Dhar, Nisha Baillie, Vicky Serafin, Natali Jones, Stephanie Madhi, Shabir A. J Virol Methods Short Communication We compared plasma and dried blood spots for detection of SARS-CoV-2 IgG antibodies. There was a good correlation between IgG values measured by both sampling methods, r = 0.935 and 0.965 for Receptor Binding Domain and full-length spike protein of SARS-CoV-2. Bland-Altman assessment showed good agreement between two sampling methods. Dried blood spots is a more pragmatic method for collecting samples for sero-epidemiological surveys of SARS-CoV-2 infection. Elsevier B.V. 2022-02 2021-11-30 /pmc/articles/PMC8631068/ /pubmed/34861318 http://dx.doi.org/10.1016/j.jviromet.2021.114394 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Kwatra, Gaurav
Nunes, Marta
Dhar, Nisha
Baillie, Vicky
Serafin, Natali
Jones, Stephanie
Madhi, Shabir A.
Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2
title Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2
title_full Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2
title_fullStr Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2
title_full_unstemmed Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2
title_short Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2
title_sort correlation of dried blood spots and plasma for quantification of immunoglobulin (igg) against receptor binding domain and full length spike protein of sars-cov-2
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631068/
https://www.ncbi.nlm.nih.gov/pubmed/34861318
http://dx.doi.org/10.1016/j.jviromet.2021.114394
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