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Pre-Existing Oxidative Stress Creates a Docking-Ready Conformation of the SARS-CoV-2 Receptor-Binding Domain
[Image: see text] The redox-dependent changes on the binding between the receptor-binding domain of the severe acute respiratory syndrome-coronavirus-2 spike protein and the peptidase domain of the human cell surface receptor angiotensin-converting enzyme II were investigated by performing molecular...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631169/ https://www.ncbi.nlm.nih.gov/pubmed/37155555 http://dx.doi.org/10.1021/acsbiomedchemau.1c00040 |
Sumario: | [Image: see text] The redox-dependent changes on the binding between the receptor-binding domain of the severe acute respiratory syndrome-coronavirus-2 spike protein and the peptidase domain of the human cell surface receptor angiotensin-converting enzyme II were investigated by performing molecular dynamics simulations. The reduced states of the protein partners were generated in silico by converting the disulfides to thiols. The role of redox transformation on the protein–protein binding affinity was assessed from the time-evolved structures after 200 ns simulations using electrostatic field calculations and implicit solvation. The present simulations revealed that the bending motion at the protein–protein interface is significantly altered when the disulfides are reduced to thiols. In the native complex, the presence of disulfide bonds preserves the structural complementarity of the protein partners and maintains the intrinsic conformational dynamics. Also, the study demonstrates that when already bound, the disulfide-to-thiol conversion of the receptor-binding domain has a limited impact on the binding of the spike protein to the receptor. However, if the reduction occurs before binding to the receptor, a spectacular conformational change of the receptor-binding domain occurs that fully impairs the binding. In other words, the formation of disulfide bonds, prevalent during oxidative stress, creates a conformation ready to bind to the receptor. Taken together, the present study demonstrates the role of pre-existing oxidative stress in elevating the binding affinity of the spike protein for the human receptor, offering future clues for alternate therapeutic possibilities. |
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