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Effective Genome Editing in Leishmania (Viannia) braziliensis Stably Expressing Cas9 and T7 RNA Polymerase

Until 2015, loss-of-function studies to elucidate protein function in Leishmania relied on gene disruption through homologous recombination. Then, the CRISPR/Cas9 revolution reached these protozoan parasites allowing efficient genome editing with one round of transfection. In addition, the developme...

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Detalles Bibliográficos
Autores principales:	Espada, Caroline R., Quilles, José Carlos, Albuquerque-Wendt, Andreia, Cruz, Mario C., Beneke, Tom, Lorenzon, Lucas B., Gluenz, Eva, Cruz, Angela K., Uliana, Silvia R. B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Cellular and Infection Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631273/ https://www.ncbi.nlm.nih.gov/pubmed/34858879 http://dx.doi.org/10.3389/fcimb.2021.772311

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