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MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response

Viral infections seriously affect the health of organisms including humans. Now, more and more researchers believe that microRNAs (miRNAs), one of the members of the non-coding RNA family, play significant roles in cell biological function, disease occurrence, and immunotherapy. However, the roles o...

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Autores principales: Li, Pin-Hong, Wang, Li-Qun, He, Jia-Yang, Zhu, Xiang-Long, Huang, Wei, Wang, Shao-Wen, Qin, Qi-Wei, Sun, Hong-Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631330/
https://www.ncbi.nlm.nih.gov/pubmed/34858424
http://dx.doi.org/10.3389/fimmu.2021.767813
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author Li, Pin-Hong
Wang, Li-Qun
He, Jia-Yang
Zhu, Xiang-Long
Huang, Wei
Wang, Shao-Wen
Qin, Qi-Wei
Sun, Hong-Yan
author_facet Li, Pin-Hong
Wang, Li-Qun
He, Jia-Yang
Zhu, Xiang-Long
Huang, Wei
Wang, Shao-Wen
Qin, Qi-Wei
Sun, Hong-Yan
author_sort Li, Pin-Hong
collection PubMed
description Viral infections seriously affect the health of organisms including humans. Now, more and more researchers believe that microRNAs (miRNAs), one of the members of the non-coding RNA family, play significant roles in cell biological function, disease occurrence, and immunotherapy. However, the roles of miRNAs in virus infection (entry and replication) and cellular immune response remain poorly understood, especially in low vertebrate fish. In this study, based on the established virus-cell infection model, Singapore grouper iridovirus (SGIV)-infected cells were used to explore the roles of miR-124 of Epinephelus coioides, an economically mariculture fish in southern China and Southeast Asia, in viral infection and host immune responses. The expression level of E. coioides miR-124 was significantly upregulated after SGIV infection; miR-124 cannot significantly affect the entry of SGIV, but the upregulated miR-124 could significantly promote the SGIV-induced cytopathic effects (CPEs), the viral titer, and the expressions of viral genes. The target genes of miR-124 were JNK3/p38α mitogen-activated protein kinase (MAPK). Overexpression of miR-124 could dramatically inhibit the activation of NF-κB/activating protein-1 (AP-1), the transcription of proinflammatory factors, caspase-9/3, and the cell apoptosis. And opposite results happen when the expression of miR-124 was inhibited. The results suggest that E. coioides miR-124 could promote viral replication and negatively regulate host immune response by targeting JNK3/p38α MAPK, which furthers our understanding of virus and host immune interactions.
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spelling pubmed-86313302021-12-01 MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response Li, Pin-Hong Wang, Li-Qun He, Jia-Yang Zhu, Xiang-Long Huang, Wei Wang, Shao-Wen Qin, Qi-Wei Sun, Hong-Yan Front Immunol Immunology Viral infections seriously affect the health of organisms including humans. Now, more and more researchers believe that microRNAs (miRNAs), one of the members of the non-coding RNA family, play significant roles in cell biological function, disease occurrence, and immunotherapy. However, the roles of miRNAs in virus infection (entry and replication) and cellular immune response remain poorly understood, especially in low vertebrate fish. In this study, based on the established virus-cell infection model, Singapore grouper iridovirus (SGIV)-infected cells were used to explore the roles of miR-124 of Epinephelus coioides, an economically mariculture fish in southern China and Southeast Asia, in viral infection and host immune responses. The expression level of E. coioides miR-124 was significantly upregulated after SGIV infection; miR-124 cannot significantly affect the entry of SGIV, but the upregulated miR-124 could significantly promote the SGIV-induced cytopathic effects (CPEs), the viral titer, and the expressions of viral genes. The target genes of miR-124 were JNK3/p38α mitogen-activated protein kinase (MAPK). Overexpression of miR-124 could dramatically inhibit the activation of NF-κB/activating protein-1 (AP-1), the transcription of proinflammatory factors, caspase-9/3, and the cell apoptosis. And opposite results happen when the expression of miR-124 was inhibited. The results suggest that E. coioides miR-124 could promote viral replication and negatively regulate host immune response by targeting JNK3/p38α MAPK, which furthers our understanding of virus and host immune interactions. Frontiers Media S.A. 2021-11-10 /pmc/articles/PMC8631330/ /pubmed/34858424 http://dx.doi.org/10.3389/fimmu.2021.767813 Text en Copyright © 2021 Li, Wang, He, Zhu, Huang, Wang, Qin and Sun https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Li, Pin-Hong
Wang, Li-Qun
He, Jia-Yang
Zhu, Xiang-Long
Huang, Wei
Wang, Shao-Wen
Qin, Qi-Wei
Sun, Hong-Yan
MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title_full MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title_fullStr MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title_full_unstemmed MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title_short MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title_sort microrna-124 promotes singapore grouper iridovirus replication and negatively regulates innate immune response
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631330/
https://www.ncbi.nlm.nih.gov/pubmed/34858424
http://dx.doi.org/10.3389/fimmu.2021.767813
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