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Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol pho...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631506/ https://www.ncbi.nlm.nih.gov/pubmed/34858957 http://dx.doi.org/10.3389/fbioe.2021.752024 |
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author | Khazi, Mahammed Ilyas Li, Chenshuo Liaqat, Fakhra Malec, Przemyslaw Li, Jian Fu, Pengcheng |
author_facet | Khazi, Mahammed Ilyas Li, Chenshuo Liaqat, Fakhra Malec, Przemyslaw Li, Jian Fu, Pengcheng |
author_sort | Khazi, Mahammed Ilyas |
collection | PubMed |
description | This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m(−2) s(−1) photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m(−2) s(−1). Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A (620)/A (280) ratio >0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A (620)/A (280) ratio >3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production. |
format | Online Article Text |
id | pubmed-8631506 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86315062021-12-01 Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production Khazi, Mahammed Ilyas Li, Chenshuo Liaqat, Fakhra Malec, Przemyslaw Li, Jian Fu, Pengcheng Front Bioeng Biotechnol Bioengineering and Biotechnology This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m(−2) s(−1) photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m(−2) s(−1). Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A (620)/A (280) ratio >0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A (620)/A (280) ratio >3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production. Frontiers Media S.A. 2021-11-10 /pmc/articles/PMC8631506/ /pubmed/34858957 http://dx.doi.org/10.3389/fbioe.2021.752024 Text en Copyright © 2021 Khazi, Li, Liaqat, Malec, Li and Fu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Khazi, Mahammed Ilyas Li, Chenshuo Liaqat, Fakhra Malec, Przemyslaw Li, Jian Fu, Pengcheng Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production |
title | Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production |
title_full | Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production |
title_fullStr | Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production |
title_full_unstemmed | Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production |
title_short | Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production |
title_sort | acclimation and characterization of marine cyanobacterial strains euryhalinema and desertifilum for c-phycocyanin production |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631506/ https://www.ncbi.nlm.nih.gov/pubmed/34858957 http://dx.doi.org/10.3389/fbioe.2021.752024 |
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