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Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production

This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol pho...

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Autores principales: Khazi, Mahammed Ilyas, Li, Chenshuo, Liaqat, Fakhra, Malec, Przemyslaw, Li, Jian, Fu, Pengcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631506/
https://www.ncbi.nlm.nih.gov/pubmed/34858957
http://dx.doi.org/10.3389/fbioe.2021.752024
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author Khazi, Mahammed Ilyas
Li, Chenshuo
Liaqat, Fakhra
Malec, Przemyslaw
Li, Jian
Fu, Pengcheng
author_facet Khazi, Mahammed Ilyas
Li, Chenshuo
Liaqat, Fakhra
Malec, Przemyslaw
Li, Jian
Fu, Pengcheng
author_sort Khazi, Mahammed Ilyas
collection PubMed
description This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m(−2) s(−1) photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m(−2) s(−1). Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A (620)/A (280) ratio >0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A (620)/A (280) ratio >3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production.
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spelling pubmed-86315062021-12-01 Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production Khazi, Mahammed Ilyas Li, Chenshuo Liaqat, Fakhra Malec, Przemyslaw Li, Jian Fu, Pengcheng Front Bioeng Biotechnol Bioengineering and Biotechnology This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m(−2) s(−1) photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m(−2) s(−1). Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A (620)/A (280) ratio >0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A (620)/A (280) ratio >3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production. Frontiers Media S.A. 2021-11-10 /pmc/articles/PMC8631506/ /pubmed/34858957 http://dx.doi.org/10.3389/fbioe.2021.752024 Text en Copyright © 2021 Khazi, Li, Liaqat, Malec, Li and Fu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Khazi, Mahammed Ilyas
Li, Chenshuo
Liaqat, Fakhra
Malec, Przemyslaw
Li, Jian
Fu, Pengcheng
Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title_full Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title_fullStr Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title_full_unstemmed Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title_short Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title_sort acclimation and characterization of marine cyanobacterial strains euryhalinema and desertifilum for c-phycocyanin production
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631506/
https://www.ncbi.nlm.nih.gov/pubmed/34858957
http://dx.doi.org/10.3389/fbioe.2021.752024
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