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Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber

Cashmere fineness is one of the important factors determining cashmere quality; however, our understanding of the regulation of cashmere fineness at the cellular level is limited. Here, we used single-cell RNA sequencing and computational models to identify 13 skin cell types in Liaoning cashmere go...

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Autores principales: Wang, Zeying, Wang, Yanru, Hui, Taiyu, Chen, Rui, Xu, Yanan, Zhang, Yu, Tian, He, Wang, Wei, Cong, Yuyan, Guo, Suping, Zhu, Yanxu, Zhang, Xinghui, Guo, Dan, Bai, Man, Fan, Yixing, Yue, Chang, Bai, Zhixian, Sun, Jiaming, Cai, Weidong, Zhang, Xinjiang, Gu, Ming, Qin, Yuting, Sun, Yinggang, Wu, Yanzhi, Wu, Rina, Dou, Xingtang, Bai, Wenlin, Zheng, Yuanyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631524/
https://www.ncbi.nlm.nih.gov/pubmed/34858469
http://dx.doi.org/10.3389/fgene.2021.726670
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author Wang, Zeying
Wang, Yanru
Hui, Taiyu
Chen, Rui
Xu, Yanan
Zhang, Yu
Tian, He
Wang, Wei
Cong, Yuyan
Guo, Suping
Zhu, Yanxu
Zhang, Xinghui
Guo, Dan
Bai, Man
Fan, Yixing
Yue, Chang
Bai, Zhixian
Sun, Jiaming
Cai, Weidong
Zhang, Xinjiang
Gu, Ming
Qin, Yuting
Sun, Yinggang
Wu, Yanzhi
Wu, Rina
Dou, Xingtang
Bai, Wenlin
Zheng, Yuanyuan
author_facet Wang, Zeying
Wang, Yanru
Hui, Taiyu
Chen, Rui
Xu, Yanan
Zhang, Yu
Tian, He
Wang, Wei
Cong, Yuyan
Guo, Suping
Zhu, Yanxu
Zhang, Xinghui
Guo, Dan
Bai, Man
Fan, Yixing
Yue, Chang
Bai, Zhixian
Sun, Jiaming
Cai, Weidong
Zhang, Xinjiang
Gu, Ming
Qin, Yuting
Sun, Yinggang
Wu, Yanzhi
Wu, Rina
Dou, Xingtang
Bai, Wenlin
Zheng, Yuanyuan
author_sort Wang, Zeying
collection PubMed
description Cashmere fineness is one of the important factors determining cashmere quality; however, our understanding of the regulation of cashmere fineness at the cellular level is limited. Here, we used single-cell RNA sequencing and computational models to identify 13 skin cell types in Liaoning cashmere goats. We also analyzed the molecular changes in the development process by cell trajectory analysis and revealed the maturation process in the gene expression profile in Liaoning cashmere goats. Weighted gene co-expression network analysis explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2, a marker gene of SDPCs, was selected for immunofluorescence (IF) and Western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results show different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify and prove the differential expression in the coarse and fine types of Liaoning cashmere goats. Therefore, the CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat SDPCs; our research provides new insights into the mechanism of cashmere growth and fineness regulation by cells.
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spelling pubmed-86315242021-12-01 Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber Wang, Zeying Wang, Yanru Hui, Taiyu Chen, Rui Xu, Yanan Zhang, Yu Tian, He Wang, Wei Cong, Yuyan Guo, Suping Zhu, Yanxu Zhang, Xinghui Guo, Dan Bai, Man Fan, Yixing Yue, Chang Bai, Zhixian Sun, Jiaming Cai, Weidong Zhang, Xinjiang Gu, Ming Qin, Yuting Sun, Yinggang Wu, Yanzhi Wu, Rina Dou, Xingtang Bai, Wenlin Zheng, Yuanyuan Front Genet Genetics Cashmere fineness is one of the important factors determining cashmere quality; however, our understanding of the regulation of cashmere fineness at the cellular level is limited. Here, we used single-cell RNA sequencing and computational models to identify 13 skin cell types in Liaoning cashmere goats. We also analyzed the molecular changes in the development process by cell trajectory analysis and revealed the maturation process in the gene expression profile in Liaoning cashmere goats. Weighted gene co-expression network analysis explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2, a marker gene of SDPCs, was selected for immunofluorescence (IF) and Western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results show different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify and prove the differential expression in the coarse and fine types of Liaoning cashmere goats. Therefore, the CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat SDPCs; our research provides new insights into the mechanism of cashmere growth and fineness regulation by cells. Frontiers Media S.A. 2021-11-10 /pmc/articles/PMC8631524/ /pubmed/34858469 http://dx.doi.org/10.3389/fgene.2021.726670 Text en Copyright © 2021 Wang, Wang, Hui, Chen, Xu, Zhang, Tian, Wang, Cong, Guo, Zhu, Zhang, Guo, Bai, Fan, Yue, Bai, Sun, Cai, Zhang, Gu, Qin, Sun, Wu, Wu, Dou, Bai and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Wang, Zeying
Wang, Yanru
Hui, Taiyu
Chen, Rui
Xu, Yanan
Zhang, Yu
Tian, He
Wang, Wei
Cong, Yuyan
Guo, Suping
Zhu, Yanxu
Zhang, Xinghui
Guo, Dan
Bai, Man
Fan, Yixing
Yue, Chang
Bai, Zhixian
Sun, Jiaming
Cai, Weidong
Zhang, Xinjiang
Gu, Ming
Qin, Yuting
Sun, Yinggang
Wu, Yanzhi
Wu, Rina
Dou, Xingtang
Bai, Wenlin
Zheng, Yuanyuan
Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber
title Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber
title_full Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber
title_fullStr Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber
title_full_unstemmed Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber
title_short Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber
title_sort single-cell sequencing reveals differential cell types in skin tissues of liaoning cashmere goats and key genes related potentially to the fineness of cashmere fiber
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631524/
https://www.ncbi.nlm.nih.gov/pubmed/34858469
http://dx.doi.org/10.3389/fgene.2021.726670
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