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Genome-wide identification and expression profiling of durian CYPome related to fruit ripening

Durian (Durio zibethinus L.) is a major economic crop native to Southeast Asian countries, including Thailand. Accordingly, understanding durian fruit ripening is an important factor in its market worldwide, owing to the fact that it is a climacteric fruit with a strikingly limited shelf life. Howev...

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Autores principales: Suntichaikamolkul, Nithiwat, Sangpong, Lalida, Schaller, Hubert, Sirikantaramas, Supaart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631664/
https://www.ncbi.nlm.nih.gov/pubmed/34847184
http://dx.doi.org/10.1371/journal.pone.0260665
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author Suntichaikamolkul, Nithiwat
Sangpong, Lalida
Schaller, Hubert
Sirikantaramas, Supaart
author_facet Suntichaikamolkul, Nithiwat
Sangpong, Lalida
Schaller, Hubert
Sirikantaramas, Supaart
author_sort Suntichaikamolkul, Nithiwat
collection PubMed
description Durian (Durio zibethinus L.) is a major economic crop native to Southeast Asian countries, including Thailand. Accordingly, understanding durian fruit ripening is an important factor in its market worldwide, owing to the fact that it is a climacteric fruit with a strikingly limited shelf life. However, knowledge regarding the molecular regulation of durian fruit ripening is still limited. Herein, we focused on cytochrome P450, a large enzyme family that regulates many biosynthetic pathways of plant metabolites and phytohormones. Deep mining of the durian genome and transcriptome libraries led to the identification of all P450s that are potentially involved in durian fruit ripening. Gene expression validation by RT-qPCR showed a high correlation with the transcriptome libraries at five fruit ripening stages. In addition to aril-specific and ripening-associated expression patterns, putative P450s that are potentially involved in phytohormone metabolism were selected for further study. Accordingly, the expression of CYP72, CYP83, CYP88, CYP94, CYP707, and CYP714 was significantly modulated by external treatment with ripening regulators, suggesting possible crosstalk between phytohormones during the regulation of fruit ripening. Interestingly, the expression levels of CYP88, CYP94, and CYP707, which are possibly involved in gibberellin, jasmonic acid, and abscisic acid biosynthesis, respectively, were significantly different between fast- and slow-post-harvest ripening cultivars, strongly implying important roles of these hormones in fruit ripening. Taken together, these phytohormone-associated P450s are potentially considered additional molecular regulators controlling ripening processes, besides ethylene and auxin, and are economically important biological traits.
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spelling pubmed-86316642021-12-01 Genome-wide identification and expression profiling of durian CYPome related to fruit ripening Suntichaikamolkul, Nithiwat Sangpong, Lalida Schaller, Hubert Sirikantaramas, Supaart PLoS One Research Article Durian (Durio zibethinus L.) is a major economic crop native to Southeast Asian countries, including Thailand. Accordingly, understanding durian fruit ripening is an important factor in its market worldwide, owing to the fact that it is a climacteric fruit with a strikingly limited shelf life. However, knowledge regarding the molecular regulation of durian fruit ripening is still limited. Herein, we focused on cytochrome P450, a large enzyme family that regulates many biosynthetic pathways of plant metabolites and phytohormones. Deep mining of the durian genome and transcriptome libraries led to the identification of all P450s that are potentially involved in durian fruit ripening. Gene expression validation by RT-qPCR showed a high correlation with the transcriptome libraries at five fruit ripening stages. In addition to aril-specific and ripening-associated expression patterns, putative P450s that are potentially involved in phytohormone metabolism were selected for further study. Accordingly, the expression of CYP72, CYP83, CYP88, CYP94, CYP707, and CYP714 was significantly modulated by external treatment with ripening regulators, suggesting possible crosstalk between phytohormones during the regulation of fruit ripening. Interestingly, the expression levels of CYP88, CYP94, and CYP707, which are possibly involved in gibberellin, jasmonic acid, and abscisic acid biosynthesis, respectively, were significantly different between fast- and slow-post-harvest ripening cultivars, strongly implying important roles of these hormones in fruit ripening. Taken together, these phytohormone-associated P450s are potentially considered additional molecular regulators controlling ripening processes, besides ethylene and auxin, and are economically important biological traits. Public Library of Science 2021-11-30 /pmc/articles/PMC8631664/ /pubmed/34847184 http://dx.doi.org/10.1371/journal.pone.0260665 Text en © 2021 Suntichaikamolkul et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Suntichaikamolkul, Nithiwat
Sangpong, Lalida
Schaller, Hubert
Sirikantaramas, Supaart
Genome-wide identification and expression profiling of durian CYPome related to fruit ripening
title Genome-wide identification and expression profiling of durian CYPome related to fruit ripening
title_full Genome-wide identification and expression profiling of durian CYPome related to fruit ripening
title_fullStr Genome-wide identification and expression profiling of durian CYPome related to fruit ripening
title_full_unstemmed Genome-wide identification and expression profiling of durian CYPome related to fruit ripening
title_short Genome-wide identification and expression profiling of durian CYPome related to fruit ripening
title_sort genome-wide identification and expression profiling of durian cypome related to fruit ripening
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631664/
https://www.ncbi.nlm.nih.gov/pubmed/34847184
http://dx.doi.org/10.1371/journal.pone.0260665
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