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Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease
Adherent invasive Escherichia Coli (AIEC) has been implicated in the pathogenesis of Crohn’s disease (CD) in Western populations. Whether the presence of AIEC is also seen in CD populations of different genetic susceptibility and has negative impact on host microbiota ecology and therapeutics are un...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632309/ https://www.ncbi.nlm.nih.gov/pubmed/34812117 http://dx.doi.org/10.1080/19490976.2021.1994833 |
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author | Xu, Zhilu Dong, Xiangqian Yang, Keli Chevarin, Caroline Zhang, Jingwan Lin, Yu Zuo, Tao Chu, Lok Cheung Sun, Yang Zhang, Fengrui Chan, Francis Kl Sung, Joseph Jy Yu, Jun Buisson, Anthony Barnich, Nicolas Colombel, Jean-Frédéric Wong, Sunny Hei Miao, Yinglei Ng, Siew C |
author_facet | Xu, Zhilu Dong, Xiangqian Yang, Keli Chevarin, Caroline Zhang, Jingwan Lin, Yu Zuo, Tao Chu, Lok Cheung Sun, Yang Zhang, Fengrui Chan, Francis Kl Sung, Joseph Jy Yu, Jun Buisson, Anthony Barnich, Nicolas Colombel, Jean-Frédéric Wong, Sunny Hei Miao, Yinglei Ng, Siew C |
author_sort | Xu, Zhilu |
collection | PubMed |
description | Adherent invasive Escherichia Coli (AIEC) has been implicated in the pathogenesis of Crohn’s disease (CD) in Western populations. Whether the presence of AIEC is also seen in CD populations of different genetic susceptibility and has negative impact on host microbiota ecology and therapeutics are unclear. AIEC presence was assessed in ileal tissues of 60 Hong Kong Chinese patients with CD and 56 healthy subjects. Mucosa microbiota was analyzed by 16s rRNA sequencing. Impact of AIEC on the gut microbiota was determined in a mouse model. AIEC was significantly more prevalent in ileal tissues of patients with CD than controls (30% vs 7.1%). Presence of AIEC in ileal tissues was associated with more severe mucosa microbiota dysbiosis in CD with decreased diversity and lower abundance of Firmicutes including butyrate producing Roseburia and probiotic Bacillus. A random forest model predicted the presence of AIEC with area under the curve of 0.89. AIEC exacerbated dysbiosis in dextran sodium sulfate (DSS)-induced colitis mice and led to resistance to restoration of normal gut microbiota by fecal microbiota transplantation (FMT). Proportion of donor-derived bacteria in AIEC-colonized mice was significantly lower than that in uninfected mice. AIEC was prevalent and associated with severe mucosa microbiota dysbiosis in CD in Hong Kong Chinese population. The presence of AIEC impeded restoration of normal gut microbiota. AIEC may serve as a keystone bacterium in CD and impact the efficacy of FMT. |
format | Online Article Text |
id | pubmed-8632309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-86323092021-12-01 Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease Xu, Zhilu Dong, Xiangqian Yang, Keli Chevarin, Caroline Zhang, Jingwan Lin, Yu Zuo, Tao Chu, Lok Cheung Sun, Yang Zhang, Fengrui Chan, Francis Kl Sung, Joseph Jy Yu, Jun Buisson, Anthony Barnich, Nicolas Colombel, Jean-Frédéric Wong, Sunny Hei Miao, Yinglei Ng, Siew C Gut Microbes Research Paper Adherent invasive Escherichia Coli (AIEC) has been implicated in the pathogenesis of Crohn’s disease (CD) in Western populations. Whether the presence of AIEC is also seen in CD populations of different genetic susceptibility and has negative impact on host microbiota ecology and therapeutics are unclear. AIEC presence was assessed in ileal tissues of 60 Hong Kong Chinese patients with CD and 56 healthy subjects. Mucosa microbiota was analyzed by 16s rRNA sequencing. Impact of AIEC on the gut microbiota was determined in a mouse model. AIEC was significantly more prevalent in ileal tissues of patients with CD than controls (30% vs 7.1%). Presence of AIEC in ileal tissues was associated with more severe mucosa microbiota dysbiosis in CD with decreased diversity and lower abundance of Firmicutes including butyrate producing Roseburia and probiotic Bacillus. A random forest model predicted the presence of AIEC with area under the curve of 0.89. AIEC exacerbated dysbiosis in dextran sodium sulfate (DSS)-induced colitis mice and led to resistance to restoration of normal gut microbiota by fecal microbiota transplantation (FMT). Proportion of donor-derived bacteria in AIEC-colonized mice was significantly lower than that in uninfected mice. AIEC was prevalent and associated with severe mucosa microbiota dysbiosis in CD in Hong Kong Chinese population. The presence of AIEC impeded restoration of normal gut microbiota. AIEC may serve as a keystone bacterium in CD and impact the efficacy of FMT. Taylor & Francis 2021-11-23 /pmc/articles/PMC8632309/ /pubmed/34812117 http://dx.doi.org/10.1080/19490976.2021.1994833 Text en © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Xu, Zhilu Dong, Xiangqian Yang, Keli Chevarin, Caroline Zhang, Jingwan Lin, Yu Zuo, Tao Chu, Lok Cheung Sun, Yang Zhang, Fengrui Chan, Francis Kl Sung, Joseph Jy Yu, Jun Buisson, Anthony Barnich, Nicolas Colombel, Jean-Frédéric Wong, Sunny Hei Miao, Yinglei Ng, Siew C Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
title | Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
title_full | Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
title_fullStr | Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
title_full_unstemmed | Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
title_short | Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
title_sort | association of adherent-invasive escherichia coli with severe gut mucosal dysbiosis in hong kong chinese population with crohn’s disease |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632309/ https://www.ncbi.nlm.nih.gov/pubmed/34812117 http://dx.doi.org/10.1080/19490976.2021.1994833 |
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