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Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans

Amyloid-β, a hallmark of Alzheimer’s disease, forms toxic intracellular oligomers and extracellular senile plaques resulting in neuronal toxicity. Ethanol is widely consumed worldwide. Moderate ethanol consumption has numerous benefits in humans. We found that ethanol could significantly extend the...

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Autores principales: Bai, Shuju, Wang, Wenbo, Zhang, Zhiwei, Li, Mengyao, Chen, Zehan, Wang, Jiuqiao, Zhao, Yanlin, An, Lu, Wang, Yuxiang, Xing, Shu, Fu, Xueqi, Ma, Junfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632871/
https://www.ncbi.nlm.nih.gov/pubmed/34867289
http://dx.doi.org/10.3389/fnagi.2021.762659
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author Bai, Shuju
Wang, Wenbo
Zhang, Zhiwei
Li, Mengyao
Chen, Zehan
Wang, Jiuqiao
Zhao, Yanlin
An, Lu
Wang, Yuxiang
Xing, Shu
Fu, Xueqi
Ma, Junfeng
author_facet Bai, Shuju
Wang, Wenbo
Zhang, Zhiwei
Li, Mengyao
Chen, Zehan
Wang, Jiuqiao
Zhao, Yanlin
An, Lu
Wang, Yuxiang
Xing, Shu
Fu, Xueqi
Ma, Junfeng
author_sort Bai, Shuju
collection PubMed
description Amyloid-β, a hallmark of Alzheimer’s disease, forms toxic intracellular oligomers and extracellular senile plaques resulting in neuronal toxicity. Ethanol is widely consumed worldwide. Moderate ethanol consumption has numerous benefits in humans. We found that ethanol could significantly extend the lifespan of Caenorhabiditis elegans in a previous study. Based on that study, we tested the effect of ethanol on Alzheimer’s disease transgenic Caenorhabiditis elegans strain CL4176, which expresses amyloid-β1-42 peptide in body wall muscle cells. Ethanol delayed paralysis and reduced amyloid-β oligomers in Caenorhabiditis elegans worms of the CL4176 strain. Moreover, ethanol could induce the nuclear translocation of DAF-16 in the nematodes. However, in worms that were fed daf-16 RNAi bacteria, ethanol no longer delayed the paralysis. The qPCR assays showed that ethanol increases the expression of daf-16, hsf-1 and their common target genes- small heat shock protein genes. In addition, we also found that ethanol could increase lysosome mass in the CL4176 worms. In summary, our study indicated that ethanol attenuated amyloid-β toxicity in the Alzheimer’s disease model of Caenorhabiditis elegans via increasing the level of lysosomes to promote amyloid-β degradation and upregulating the levels of small heat shock protein genes to reduce amyloid-β aggregation.
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spelling pubmed-86328712021-12-02 Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans Bai, Shuju Wang, Wenbo Zhang, Zhiwei Li, Mengyao Chen, Zehan Wang, Jiuqiao Zhao, Yanlin An, Lu Wang, Yuxiang Xing, Shu Fu, Xueqi Ma, Junfeng Front Aging Neurosci Neuroscience Amyloid-β, a hallmark of Alzheimer’s disease, forms toxic intracellular oligomers and extracellular senile plaques resulting in neuronal toxicity. Ethanol is widely consumed worldwide. Moderate ethanol consumption has numerous benefits in humans. We found that ethanol could significantly extend the lifespan of Caenorhabiditis elegans in a previous study. Based on that study, we tested the effect of ethanol on Alzheimer’s disease transgenic Caenorhabiditis elegans strain CL4176, which expresses amyloid-β1-42 peptide in body wall muscle cells. Ethanol delayed paralysis and reduced amyloid-β oligomers in Caenorhabiditis elegans worms of the CL4176 strain. Moreover, ethanol could induce the nuclear translocation of DAF-16 in the nematodes. However, in worms that were fed daf-16 RNAi bacteria, ethanol no longer delayed the paralysis. The qPCR assays showed that ethanol increases the expression of daf-16, hsf-1 and their common target genes- small heat shock protein genes. In addition, we also found that ethanol could increase lysosome mass in the CL4176 worms. In summary, our study indicated that ethanol attenuated amyloid-β toxicity in the Alzheimer’s disease model of Caenorhabiditis elegans via increasing the level of lysosomes to promote amyloid-β degradation and upregulating the levels of small heat shock protein genes to reduce amyloid-β aggregation. Frontiers Media S.A. 2021-11-12 /pmc/articles/PMC8632871/ /pubmed/34867289 http://dx.doi.org/10.3389/fnagi.2021.762659 Text en Copyright © 2021 Bai, Wang, Zhang, Li, Chen, Wang, Zhao, An, Wang, Xing, Fu and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Bai, Shuju
Wang, Wenbo
Zhang, Zhiwei
Li, Mengyao
Chen, Zehan
Wang, Jiuqiao
Zhao, Yanlin
An, Lu
Wang, Yuxiang
Xing, Shu
Fu, Xueqi
Ma, Junfeng
Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title_full Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title_fullStr Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title_full_unstemmed Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title_short Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title_sort ethanol alleviates amyloid-β-induced toxicity in an alzheimer’s disease model of caenorhabiditis elegans
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632871/
https://www.ncbi.nlm.nih.gov/pubmed/34867289
http://dx.doi.org/10.3389/fnagi.2021.762659
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