Cargando…
The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry
Flower and fruit development are two key steps for plant reproduction. The ABCE model for flower development has been well established in model plant species; however, the functions of ABCE genes in fruit crops are less understood. In this work, we identified an EMS mutant named R27 in woodland stra...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632884/ https://www.ncbi.nlm.nih.gov/pubmed/34848694 http://dx.doi.org/10.1038/s41438-021-00673-1 |
_version_ | 1784607839108464640 |
---|---|
author | Pi, Mengting Hu, Shaoqiang Cheng, Laichao Zhong, Ruhan Cai, Zhuoying Liu, Zhongchi Yao, Jia-Long Kang, Chunying |
author_facet | Pi, Mengting Hu, Shaoqiang Cheng, Laichao Zhong, Ruhan Cai, Zhuoying Liu, Zhongchi Yao, Jia-Long Kang, Chunying |
author_sort | Pi, Mengting |
collection | PubMed |
description | Flower and fruit development are two key steps for plant reproduction. The ABCE model for flower development has been well established in model plant species; however, the functions of ABCE genes in fruit crops are less understood. In this work, we identified an EMS mutant named R27 in woodland strawberry (Fragaria vesca), showing the conversion of petals, stamens, and carpels to sepaloid organs in a semidominant inheritance fashion. Mapping by sequencing revealed that the class E gene homolog FveSEP3 (FvH4_4g23530) possessed the causative mutation in R27 due to a G to E amino acid change in the conserved MADS domain. Additional fvesep3(CR) mutants generated by CRISPR/Cas9 displayed similar phenotypes to fvesep3-R27. Overexpressing wild-type or mutated FveSEP3 in Arabidopsis suggested that the mutation in R27 might cause a dominant-negative effect. Further analyses indicated that FveSEP3 physically interacted with each of the ABCE proteins in strawberry. Moreover, both R27 and fvesep3(CR) mutants exhibited parthenocarpic fruit growth and delayed fruit ripening. Transcriptome analysis revealed that both common and specific differentially expressed genes were identified in young fruit at 6–7 days post anthesis (DPA) of fvesep3 and pollinated wild type when compared to unpollinated wild type, especially those in the auxin pathway, a key hormone regulating fruit set in strawberry. Together, we provided compelling evidence that FveSEP3 plays predominant E functions compared to other E gene homologs in flower development and that FveSEP3 represses fruit growth in the absence of pollination and promotes fruit ripening in strawberry. |
format | Online Article Text |
id | pubmed-8632884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-86328842021-12-01 The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry Pi, Mengting Hu, Shaoqiang Cheng, Laichao Zhong, Ruhan Cai, Zhuoying Liu, Zhongchi Yao, Jia-Long Kang, Chunying Hortic Res Article Flower and fruit development are two key steps for plant reproduction. The ABCE model for flower development has been well established in model plant species; however, the functions of ABCE genes in fruit crops are less understood. In this work, we identified an EMS mutant named R27 in woodland strawberry (Fragaria vesca), showing the conversion of petals, stamens, and carpels to sepaloid organs in a semidominant inheritance fashion. Mapping by sequencing revealed that the class E gene homolog FveSEP3 (FvH4_4g23530) possessed the causative mutation in R27 due to a G to E amino acid change in the conserved MADS domain. Additional fvesep3(CR) mutants generated by CRISPR/Cas9 displayed similar phenotypes to fvesep3-R27. Overexpressing wild-type or mutated FveSEP3 in Arabidopsis suggested that the mutation in R27 might cause a dominant-negative effect. Further analyses indicated that FveSEP3 physically interacted with each of the ABCE proteins in strawberry. Moreover, both R27 and fvesep3(CR) mutants exhibited parthenocarpic fruit growth and delayed fruit ripening. Transcriptome analysis revealed that both common and specific differentially expressed genes were identified in young fruit at 6–7 days post anthesis (DPA) of fvesep3 and pollinated wild type when compared to unpollinated wild type, especially those in the auxin pathway, a key hormone regulating fruit set in strawberry. Together, we provided compelling evidence that FveSEP3 plays predominant E functions compared to other E gene homologs in flower development and that FveSEP3 represses fruit growth in the absence of pollination and promotes fruit ripening in strawberry. Nature Publishing Group UK 2021-12-01 /pmc/articles/PMC8632884/ /pubmed/34848694 http://dx.doi.org/10.1038/s41438-021-00673-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Pi, Mengting Hu, Shaoqiang Cheng, Laichao Zhong, Ruhan Cai, Zhuoying Liu, Zhongchi Yao, Jia-Long Kang, Chunying The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry |
title | The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry |
title_full | The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry |
title_fullStr | The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry |
title_full_unstemmed | The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry |
title_short | The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry |
title_sort | mads-box gene fvesep3 plays essential roles in flower organogenesis and fruit development in woodland strawberry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632884/ https://www.ncbi.nlm.nih.gov/pubmed/34848694 http://dx.doi.org/10.1038/s41438-021-00673-1 |
work_keys_str_mv | AT pimengting themadsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT hushaoqiang themadsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT chenglaichao themadsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT zhongruhan themadsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT caizhuoying themadsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT liuzhongchi themadsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT yaojialong themadsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT kangchunying themadsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT pimengting madsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT hushaoqiang madsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT chenglaichao madsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT zhongruhan madsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT caizhuoying madsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT liuzhongchi madsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT yaojialong madsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry AT kangchunying madsboxgenefvesep3playsessentialrolesinflowerorganogenesisandfruitdevelopmentinwoodlandstrawberry |