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Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes
Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity in a narrow window upstream of RNA Polymerase (Pol) III-transcribed genes, representing a paradigm for...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632968/ https://www.ncbi.nlm.nih.gov/pubmed/34848735 http://dx.doi.org/10.1038/s41467-021-27338-w |
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author | Abascal-Palacios, Guillermo Jochem, Laura Pla-Prats, Carlos Beuron, Fabienne Vannini, Alessandro |
author_facet | Abascal-Palacios, Guillermo Jochem, Laura Pla-Prats, Carlos Beuron, Fabienne Vannini, Alessandro |
author_sort | Abascal-Palacios, Guillermo |
collection | PubMed |
description | Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity in a narrow window upstream of RNA Polymerase (Pol) III-transcribed genes, representing a paradigm for harmless targeted integration. Here we present the cryo-EM reconstruction at 4.0 Å of an active Ty3 strand transfer complex bound to TFIIIB transcription factor and a tRNA gene. The structure unravels the molecular mechanisms underlying Ty3 targeting specificity at Pol III-transcribed genes and sheds light into the architecture of retrotransposon machinery during integration. Ty3 intasome contacts a region of TBP, a subunit of TFIIIB, which is blocked by NC2 transcription regulator in RNA Pol II-transcribed genes. A newly-identified chromodomain on Ty3 integrase interacts with TFIIIB and the tRNA gene, defining with extreme precision the integration site position. |
format | Online Article Text |
id | pubmed-8632968 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-86329682021-12-15 Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes Abascal-Palacios, Guillermo Jochem, Laura Pla-Prats, Carlos Beuron, Fabienne Vannini, Alessandro Nat Commun Article Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity in a narrow window upstream of RNA Polymerase (Pol) III-transcribed genes, representing a paradigm for harmless targeted integration. Here we present the cryo-EM reconstruction at 4.0 Å of an active Ty3 strand transfer complex bound to TFIIIB transcription factor and a tRNA gene. The structure unravels the molecular mechanisms underlying Ty3 targeting specificity at Pol III-transcribed genes and sheds light into the architecture of retrotransposon machinery during integration. Ty3 intasome contacts a region of TBP, a subunit of TFIIIB, which is blocked by NC2 transcription regulator in RNA Pol II-transcribed genes. A newly-identified chromodomain on Ty3 integrase interacts with TFIIIB and the tRNA gene, defining with extreme precision the integration site position. Nature Publishing Group UK 2021-11-30 /pmc/articles/PMC8632968/ /pubmed/34848735 http://dx.doi.org/10.1038/s41467-021-27338-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Abascal-Palacios, Guillermo Jochem, Laura Pla-Prats, Carlos Beuron, Fabienne Vannini, Alessandro Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes |
title | Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes |
title_full | Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes |
title_fullStr | Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes |
title_full_unstemmed | Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes |
title_short | Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes |
title_sort | structural basis of ty3 retrotransposon integration at rna polymerase iii-transcribed genes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632968/ https://www.ncbi.nlm.nih.gov/pubmed/34848735 http://dx.doi.org/10.1038/s41467-021-27338-w |
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