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A quantitative analysis of bone lamellarity and bone collagen linearity induced by distinct dosing and frequencies of teriparatide administration in ovariectomized rats and monkeys
The lamellar structure of bone, which endows biomechanical rigidity to support the host organism, is observed in mammals, including humans. It is therefore essential to develop a quantitative analysis to evaluate the lamellarity of bone, which would especially be useful for the pharmacological evalu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633100/ https://www.ncbi.nlm.nih.gov/pubmed/34100544 http://dx.doi.org/10.1093/jmicro/dfab020 |
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author | Sato, Takanori Takakura, Aya Lee, Ji-Won Tokunaga, Kazuaki Matsumori, Haruka Takao-Kawabata, Ryoko Iimura, Tadahiro |
author_facet | Sato, Takanori Takakura, Aya Lee, Ji-Won Tokunaga, Kazuaki Matsumori, Haruka Takao-Kawabata, Ryoko Iimura, Tadahiro |
author_sort | Sato, Takanori |
collection | PubMed |
description | The lamellar structure of bone, which endows biomechanical rigidity to support the host organism, is observed in mammals, including humans. It is therefore essential to develop a quantitative analysis to evaluate the lamellarity of bone, which would especially be useful for the pharmacological evaluation of anti-osteoporotic drugs. This study applied a current system for the semi-automatic recognition of fluorescence signals to the analysis of un-decalcified bone sections from rat and monkey specimens treated with teriparatide (TPTD). Our analyses on bone formation pattern and collagen topology indicated that TPTD augmented bone lamellarity and bone collagen linearity, which were possibly associated with the recovery of collagen cross-linking, thus endowing bone rigidity. |
format | Online Article Text |
id | pubmed-8633100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-86331002021-12-01 A quantitative analysis of bone lamellarity and bone collagen linearity induced by distinct dosing and frequencies of teriparatide administration in ovariectomized rats and monkeys Sato, Takanori Takakura, Aya Lee, Ji-Won Tokunaga, Kazuaki Matsumori, Haruka Takao-Kawabata, Ryoko Iimura, Tadahiro Microscopy (Oxf) Article The lamellar structure of bone, which endows biomechanical rigidity to support the host organism, is observed in mammals, including humans. It is therefore essential to develop a quantitative analysis to evaluate the lamellarity of bone, which would especially be useful for the pharmacological evaluation of anti-osteoporotic drugs. This study applied a current system for the semi-automatic recognition of fluorescence signals to the analysis of un-decalcified bone sections from rat and monkey specimens treated with teriparatide (TPTD). Our analyses on bone formation pattern and collagen topology indicated that TPTD augmented bone lamellarity and bone collagen linearity, which were possibly associated with the recovery of collagen cross-linking, thus endowing bone rigidity. Oxford University Press 2021-06-08 /pmc/articles/PMC8633100/ /pubmed/34100544 http://dx.doi.org/10.1093/jmicro/dfab020 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Article Sato, Takanori Takakura, Aya Lee, Ji-Won Tokunaga, Kazuaki Matsumori, Haruka Takao-Kawabata, Ryoko Iimura, Tadahiro A quantitative analysis of bone lamellarity and bone collagen linearity induced by distinct dosing and frequencies of teriparatide administration in ovariectomized rats and monkeys |
title | A quantitative analysis of bone lamellarity and bone collagen linearity induced by distinct dosing and frequencies of teriparatide administration in ovariectomized rats and monkeys |
title_full | A quantitative analysis of bone lamellarity and bone collagen linearity induced by distinct dosing and frequencies of teriparatide administration in ovariectomized rats and monkeys |
title_fullStr | A quantitative analysis of bone lamellarity and bone collagen linearity induced by distinct dosing and frequencies of teriparatide administration in ovariectomized rats and monkeys |
title_full_unstemmed | A quantitative analysis of bone lamellarity and bone collagen linearity induced by distinct dosing and frequencies of teriparatide administration in ovariectomized rats and monkeys |
title_short | A quantitative analysis of bone lamellarity and bone collagen linearity induced by distinct dosing and frequencies of teriparatide administration in ovariectomized rats and monkeys |
title_sort | quantitative analysis of bone lamellarity and bone collagen linearity induced by distinct dosing and frequencies of teriparatide administration in ovariectomized rats and monkeys |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633100/ https://www.ncbi.nlm.nih.gov/pubmed/34100544 http://dx.doi.org/10.1093/jmicro/dfab020 |
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