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Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles

The gametophyte of ferns reproduces either by sexual or asexual means. In the latter, apogamy represents a peculiar case of apomixis, in which an embryo is formed from somatic cells. A proteomic and physiological approach was applied to the apogamous fern Dryopteris affinis ssp. affinis and its sexu...

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Autores principales: Fernández, Helena, Grossmann, Jonas, Gagliardini, Valeria, Feito, Isabel, Rivera, Alejandro, Rodríguez, Lucía, Quintanilla, Luis G., Quesada, Víctor, Cañal, Mª Jesús, Grossniklaus, Ueli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633544/
https://www.ncbi.nlm.nih.gov/pubmed/34868105
http://dx.doi.org/10.3389/fpls.2021.718932
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author Fernández, Helena
Grossmann, Jonas
Gagliardini, Valeria
Feito, Isabel
Rivera, Alejandro
Rodríguez, Lucía
Quintanilla, Luis G.
Quesada, Víctor
Cañal, Mª Jesús
Grossniklaus, Ueli
author_facet Fernández, Helena
Grossmann, Jonas
Gagliardini, Valeria
Feito, Isabel
Rivera, Alejandro
Rodríguez, Lucía
Quintanilla, Luis G.
Quesada, Víctor
Cañal, Mª Jesús
Grossniklaus, Ueli
author_sort Fernández, Helena
collection PubMed
description The gametophyte of ferns reproduces either by sexual or asexual means. In the latter, apogamy represents a peculiar case of apomixis, in which an embryo is formed from somatic cells. A proteomic and physiological approach was applied to the apogamous fern Dryopteris affinis ssp. affinis and its sexual relative D. oreades. The proteomic analysis compared apogamous vs. female gametophytes, whereas the phytohormone study included, in addition to females, three apogamous stages (filamentous, spatulate, and cordate). The proteomic profiles revealed a total of 879 proteins and, after annotation, different regulation was found in 206 proteins of D. affinis and 166 of its sexual counterpart. The proteins upregulated in D. affinis are mostly associated to protein metabolism (including folding, transport, and proteolysis), ribosome biogenesis, gene expression and translation, while in the sexual counterpart, they account largely for starch and sucrose metabolism, generation of energy and photosynthesis. Likewise, ultra-performance liquid chromatography-tandem spectrometry (UHPLC-MS/MS) was used to assess the levels of indol-3-acetic acid (IAA); the cytokinins: 6-benzylaminopurine (BA), trans-Zeatine (Z), trans-Zeatin riboside (ZR), dyhidrozeatine (DHZ), dyhidrozeatin riboside (DHZR), isopentenyl adenine (iP), isopentenyl adenosine (iPR), abscisic acid (ABA), the gibberellins GA(3) and GA(4), salicylic acid (SA), and the brassinosteroids: brassinolide (BL) and castasterone (CS). IAA, the cytokinins Z, ZR, iPR, the gibberellin GA(4), the brassinosteoids castasterone, and ABA accumulated more in the sexual gametophyte than in the apogamous one. When comparing the three apogamous stages, BA and SA peaked in filamentous, GA(3) and BL in spatulate and DHRZ in cordate gametophytes. The results point to the existence of large metabolic differences between apogamous and sexual gametophytes, and invite to consider the fern gametophyte as a good experimental system to deepen our understanding of plant reproduction.
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spelling pubmed-86335442021-12-02 Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles Fernández, Helena Grossmann, Jonas Gagliardini, Valeria Feito, Isabel Rivera, Alejandro Rodríguez, Lucía Quintanilla, Luis G. Quesada, Víctor Cañal, Mª Jesús Grossniklaus, Ueli Front Plant Sci Plant Science The gametophyte of ferns reproduces either by sexual or asexual means. In the latter, apogamy represents a peculiar case of apomixis, in which an embryo is formed from somatic cells. A proteomic and physiological approach was applied to the apogamous fern Dryopteris affinis ssp. affinis and its sexual relative D. oreades. The proteomic analysis compared apogamous vs. female gametophytes, whereas the phytohormone study included, in addition to females, three apogamous stages (filamentous, spatulate, and cordate). The proteomic profiles revealed a total of 879 proteins and, after annotation, different regulation was found in 206 proteins of D. affinis and 166 of its sexual counterpart. The proteins upregulated in D. affinis are mostly associated to protein metabolism (including folding, transport, and proteolysis), ribosome biogenesis, gene expression and translation, while in the sexual counterpart, they account largely for starch and sucrose metabolism, generation of energy and photosynthesis. Likewise, ultra-performance liquid chromatography-tandem spectrometry (UHPLC-MS/MS) was used to assess the levels of indol-3-acetic acid (IAA); the cytokinins: 6-benzylaminopurine (BA), trans-Zeatine (Z), trans-Zeatin riboside (ZR), dyhidrozeatine (DHZ), dyhidrozeatin riboside (DHZR), isopentenyl adenine (iP), isopentenyl adenosine (iPR), abscisic acid (ABA), the gibberellins GA(3) and GA(4), salicylic acid (SA), and the brassinosteroids: brassinolide (BL) and castasterone (CS). IAA, the cytokinins Z, ZR, iPR, the gibberellin GA(4), the brassinosteoids castasterone, and ABA accumulated more in the sexual gametophyte than in the apogamous one. When comparing the three apogamous stages, BA and SA peaked in filamentous, GA(3) and BL in spatulate and DHRZ in cordate gametophytes. The results point to the existence of large metabolic differences between apogamous and sexual gametophytes, and invite to consider the fern gametophyte as a good experimental system to deepen our understanding of plant reproduction. Frontiers Media S.A. 2021-11-12 /pmc/articles/PMC8633544/ /pubmed/34868105 http://dx.doi.org/10.3389/fpls.2021.718932 Text en Copyright © 2021 Fernández, Grossmann, Gagliardini, Feito, Rivera, Rodríguez, Quintanilla, Quesada, Cañal and Grossniklaus. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Fernández, Helena
Grossmann, Jonas
Gagliardini, Valeria
Feito, Isabel
Rivera, Alejandro
Rodríguez, Lucía
Quintanilla, Luis G.
Quesada, Víctor
Cañal, Mª Jesús
Grossniklaus, Ueli
Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles
title Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles
title_full Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles
title_fullStr Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles
title_full_unstemmed Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles
title_short Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles
title_sort sexual and apogamous species of woodferns show different protein and phytohormone profiles
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633544/
https://www.ncbi.nlm.nih.gov/pubmed/34868105
http://dx.doi.org/10.3389/fpls.2021.718932
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