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Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea
In Korea, 4 big layer companies that possess one grandparent and 3 parent stocks are in charge of 100% of the layer chicken industry. In this study, we investigated the antimicrobial resistance of commensal 578 E. coli isolated from 20 flocks of 4-layer breeder farms (A, B, C, and D), moreover, comp...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633676/ https://www.ncbi.nlm.nih.gov/pubmed/34844113 http://dx.doi.org/10.1016/j.psj.2021.101571 |
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author | Kim, Dong gyu Kim, Koeun Bae, Sung Hyun Jung, Hye-Ri Kang, Hyo Jung Lee, Yu Jin Seo, Kwang Won Lee, Young Ju |
author_facet | Kim, Dong gyu Kim, Koeun Bae, Sung Hyun Jung, Hye-Ri Kang, Hyo Jung Lee, Yu Jin Seo, Kwang Won Lee, Young Ju |
author_sort | Kim, Dong gyu |
collection | PubMed |
description | In Korea, 4 big layer companies that possess one grandparent and 3 parent stocks are in charge of 100% of the layer chicken industry. In this study, we investigated the antimicrobial resistance of commensal 578 E. coli isolated from 20 flocks of 4-layer breeder farms (A, B, C, and D), moreover, compared the characteristics of their resistance and virulence genes. Isolates from farms B and D showed significantly higher resistance to the β-lactam antimicrobials (amoxicillin, ampicillin, and 1st-, 2nd-, and 3rd-generation cephalosporins). However, resistance to ciprofloxacin, nalidixic acid, and tetracycline was significantly higher in the isolates from farm A (P < 0.05). Interestingly, the isolates from farm C showed significantly lower resistance to most antimicrobials tested in this study. The isolates from farms B, C, and D showed the high multiple resistance to the 3 antimicrobial classes. Furthermore, the isolates from farm A showed the highest multiple resistance against the 5 classes. Among the 412 β-lactam-resistant isolates, 123 (29.9%) carried bla(TEM-1), but the distribution was significantly different among the farms from 17.5% to 51.4% (P < 0.05). Similarly, the most prevalent tetracycline resistance gene in the isolates from farms B, C, and D was tetA (50.0–77.0%); however, the isolates from farm A showed the highest prevalence in tetB (70.6%). The distribution of quinolone (qnrB, qnrD, and qnrS) and sulfonamide (su12)-resistant genes were also significantly different among the farms but that of chloramphenicol (catA1)- and aminoglycoside (aac [3]-II, and aac [6′]-Ib)-resistant genes possessed no significant difference among the farms. Moreover, the isolates from farm C showed significantly higher prevalence in virulence genes (iroN, ompT, hlyF, and iss) than the other 3 farms (P < 0.05). Furthermore, the phenotypic and genotypic characteristics of E. coli isolates were significantly different among the farms, and improved management protocols are required to control of horizontal and vertical transmission of avian disease, including the dissemination of resistant bacteria in breeder flocks. |
format | Online Article Text |
id | pubmed-8633676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-86336762021-12-06 Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea Kim, Dong gyu Kim, Koeun Bae, Sung Hyun Jung, Hye-Ri Kang, Hyo Jung Lee, Yu Jin Seo, Kwang Won Lee, Young Ju Poult Sci MICROBIOLOGY AND FOOD SAFETY In Korea, 4 big layer companies that possess one grandparent and 3 parent stocks are in charge of 100% of the layer chicken industry. In this study, we investigated the antimicrobial resistance of commensal 578 E. coli isolated from 20 flocks of 4-layer breeder farms (A, B, C, and D), moreover, compared the characteristics of their resistance and virulence genes. Isolates from farms B and D showed significantly higher resistance to the β-lactam antimicrobials (amoxicillin, ampicillin, and 1st-, 2nd-, and 3rd-generation cephalosporins). However, resistance to ciprofloxacin, nalidixic acid, and tetracycline was significantly higher in the isolates from farm A (P < 0.05). Interestingly, the isolates from farm C showed significantly lower resistance to most antimicrobials tested in this study. The isolates from farms B, C, and D showed the high multiple resistance to the 3 antimicrobial classes. Furthermore, the isolates from farm A showed the highest multiple resistance against the 5 classes. Among the 412 β-lactam-resistant isolates, 123 (29.9%) carried bla(TEM-1), but the distribution was significantly different among the farms from 17.5% to 51.4% (P < 0.05). Similarly, the most prevalent tetracycline resistance gene in the isolates from farms B, C, and D was tetA (50.0–77.0%); however, the isolates from farm A showed the highest prevalence in tetB (70.6%). The distribution of quinolone (qnrB, qnrD, and qnrS) and sulfonamide (su12)-resistant genes were also significantly different among the farms but that of chloramphenicol (catA1)- and aminoglycoside (aac [3]-II, and aac [6′]-Ib)-resistant genes possessed no significant difference among the farms. Moreover, the isolates from farm C showed significantly higher prevalence in virulence genes (iroN, ompT, hlyF, and iss) than the other 3 farms (P < 0.05). Furthermore, the phenotypic and genotypic characteristics of E. coli isolates were significantly different among the farms, and improved management protocols are required to control of horizontal and vertical transmission of avian disease, including the dissemination of resistant bacteria in breeder flocks. Elsevier 2021-10-29 /pmc/articles/PMC8633676/ /pubmed/34844113 http://dx.doi.org/10.1016/j.psj.2021.101571 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | MICROBIOLOGY AND FOOD SAFETY Kim, Dong gyu Kim, Koeun Bae, Sung Hyun Jung, Hye-Ri Kang, Hyo Jung Lee, Yu Jin Seo, Kwang Won Lee, Young Ju Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea |
title | Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea |
title_full | Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea |
title_fullStr | Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea |
title_full_unstemmed | Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea |
title_short | Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea |
title_sort | comparison of antimicrobial resistance and molecular characterization of escherichia coli isolates from layer breeder farms in korea |
topic | MICROBIOLOGY AND FOOD SAFETY |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633676/ https://www.ncbi.nlm.nih.gov/pubmed/34844113 http://dx.doi.org/10.1016/j.psj.2021.101571 |
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