Cargando…

codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells

Staphylococcus epidermidis biofilm cells can enter a physiological state known as viable but non-culturable (VBNC), where, despite being alive, they do not grow in conventional laboratory media. As such, the presence of VBNC cells impacts the diagnosis of S. epidermidis biofilm-associated infections...

Descripción completa

Detalles Bibliográficos
Autores principales: Gaio, Vânia, Lopes, Nathalie, Cerca, Nuno, França, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634161/
https://www.ncbi.nlm.nih.gov/pubmed/34869073
http://dx.doi.org/10.3389/fcimb.2021.771666
_version_ 1784608080445571072
author Gaio, Vânia
Lopes, Nathalie
Cerca, Nuno
França, Angela
author_facet Gaio, Vânia
Lopes, Nathalie
Cerca, Nuno
França, Angela
author_sort Gaio, Vânia
collection PubMed
description Staphylococcus epidermidis biofilm cells can enter a physiological state known as viable but non-culturable (VBNC), where, despite being alive, they do not grow in conventional laboratory media. As such, the presence of VBNC cells impacts the diagnosis of S. epidermidis biofilm-associated infections. Previous transcriptomics analysis of S. epidermidis strain 9142 biofilms with higher proportions of VBNC cells suggested that the genes pdhA, codY and mazEF could be involved in the induction of the VBNC state. However, it was previously demonstrated that VBNC induction is strain-dependent. To properly assess the role of these genes in VBNC induction, the construction of mutant strains is necessary. Thus, herein, we assessed if VBNC cells could be induced in strain 1457, a strain amenable to genetic manipulation, and if the previously identified genes were involved in the modulation of the VBNC state in this strain. Furthermore, we evaluated the formation of VBNC cells on planktonic cultures. Our results showed that despite being commonly associated with biofilms, the proportion of VBNC cells can be modulated in both biofilm and planktonic cultures and that the expression of codY and pdhA was upregulated under VBNC inducing conditions in both phenotypes. Overall, our study revealed that the formation of VBNC cells in S. epidermidis is independent of the mode of growth and that the genes codY and pdhA seem to be relevant for the regulation of this physiological condition.
format Online
Article
Text
id pubmed-8634161
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86341612021-12-02 codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells Gaio, Vânia Lopes, Nathalie Cerca, Nuno França, Angela Front Cell Infect Microbiol Cellular and Infection Microbiology Staphylococcus epidermidis biofilm cells can enter a physiological state known as viable but non-culturable (VBNC), where, despite being alive, they do not grow in conventional laboratory media. As such, the presence of VBNC cells impacts the diagnosis of S. epidermidis biofilm-associated infections. Previous transcriptomics analysis of S. epidermidis strain 9142 biofilms with higher proportions of VBNC cells suggested that the genes pdhA, codY and mazEF could be involved in the induction of the VBNC state. However, it was previously demonstrated that VBNC induction is strain-dependent. To properly assess the role of these genes in VBNC induction, the construction of mutant strains is necessary. Thus, herein, we assessed if VBNC cells could be induced in strain 1457, a strain amenable to genetic manipulation, and if the previously identified genes were involved in the modulation of the VBNC state in this strain. Furthermore, we evaluated the formation of VBNC cells on planktonic cultures. Our results showed that despite being commonly associated with biofilms, the proportion of VBNC cells can be modulated in both biofilm and planktonic cultures and that the expression of codY and pdhA was upregulated under VBNC inducing conditions in both phenotypes. Overall, our study revealed that the formation of VBNC cells in S. epidermidis is independent of the mode of growth and that the genes codY and pdhA seem to be relevant for the regulation of this physiological condition. Frontiers Media S.A. 2021-11-15 /pmc/articles/PMC8634161/ /pubmed/34869073 http://dx.doi.org/10.3389/fcimb.2021.771666 Text en Copyright © 2021 Gaio, Lopes, Cerca and França https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Gaio, Vânia
Lopes, Nathalie
Cerca, Nuno
França, Angela
codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title_full codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title_fullStr codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title_full_unstemmed codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title_short codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title_sort cody and pdha expression is induced in staphylococcus epidermidis biofilm and planktonic populations with higher proportions of viable but non-culturable cells
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634161/
https://www.ncbi.nlm.nih.gov/pubmed/34869073
http://dx.doi.org/10.3389/fcimb.2021.771666
work_keys_str_mv AT gaiovania codyandpdhaexpressionisinducedinstaphylococcusepidermidisbiofilmandplanktonicpopulationswithhigherproportionsofviablebutnonculturablecells
AT lopesnathalie codyandpdhaexpressionisinducedinstaphylococcusepidermidisbiofilmandplanktonicpopulationswithhigherproportionsofviablebutnonculturablecells
AT cercanuno codyandpdhaexpressionisinducedinstaphylococcusepidermidisbiofilmandplanktonicpopulationswithhigherproportionsofviablebutnonculturablecells
AT francaangela codyandpdhaexpressionisinducedinstaphylococcusepidermidisbiofilmandplanktonicpopulationswithhigherproportionsofviablebutnonculturablecells