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First report of Seville root-knot nematode, Meloidogyne hispanica (Nematoda: Meloidogynidae) in the USA and North America

A high number of second stage juveniles of the root-knot nematode were recovered from soil samples collected from a corn field, located in Pickens County, South Carolina, USA in 2019. Extracted nematodes were examined morphologically and molecularly for species identification which indicated that th...

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Autores principales: Skantar, Andrea M., Handoo, Zafar A., Subbotin, Sergei A., Kantor, Mihail R., Vieira, Paulo, Agudelo, Paula, Hult, Maria N., Rogers, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Exeley Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634290/
https://www.ncbi.nlm.nih.gov/pubmed/34881368
http://dx.doi.org/10.21307/jofnem-2021-098
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author Skantar, Andrea M.
Handoo, Zafar A.
Subbotin, Sergei A.
Kantor, Mihail R.
Vieira, Paulo
Agudelo, Paula
Hult, Maria N.
Rogers, Stephen
author_facet Skantar, Andrea M.
Handoo, Zafar A.
Subbotin, Sergei A.
Kantor, Mihail R.
Vieira, Paulo
Agudelo, Paula
Hult, Maria N.
Rogers, Stephen
author_sort Skantar, Andrea M.
collection PubMed
description A high number of second stage juveniles of the root-knot nematode were recovered from soil samples collected from a corn field, located in Pickens County, South Carolina, USA in 2019. Extracted nematodes were examined morphologically and molecularly for species identification which indicated that the specimens of root knot juveniles were Meloidogyne hispanica. The morphological examination and morphometric details from second-stage juveniles were consistent with the original description and redescriptions of this species. The ITS rRNA, D2-D3 expansion segments of 28S rRNA, intergenic COII-16S region, nad5 and COI gene sequences were obtained from the South Carolina population of M. hispanica. Phylogenetic analysis of the intergenic COII-16S region of mtDNA gene sequence alignment using statistical parsimony showed that the South Carolina population clustered with Meloidogyne hispanica from Portugal and Australia. To our best knowledge, this finding represents the first report of Meloidogyne hispanica in the USA and North America.
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spelling pubmed-86342902021-12-07 First report of Seville root-knot nematode, Meloidogyne hispanica (Nematoda: Meloidogynidae) in the USA and North America Skantar, Andrea M. Handoo, Zafar A. Subbotin, Sergei A. Kantor, Mihail R. Vieira, Paulo Agudelo, Paula Hult, Maria N. Rogers, Stephen J Nematol Life Sciences A high number of second stage juveniles of the root-knot nematode were recovered from soil samples collected from a corn field, located in Pickens County, South Carolina, USA in 2019. Extracted nematodes were examined morphologically and molecularly for species identification which indicated that the specimens of root knot juveniles were Meloidogyne hispanica. The morphological examination and morphometric details from second-stage juveniles were consistent with the original description and redescriptions of this species. The ITS rRNA, D2-D3 expansion segments of 28S rRNA, intergenic COII-16S region, nad5 and COI gene sequences were obtained from the South Carolina population of M. hispanica. Phylogenetic analysis of the intergenic COII-16S region of mtDNA gene sequence alignment using statistical parsimony showed that the South Carolina population clustered with Meloidogyne hispanica from Portugal and Australia. To our best knowledge, this finding represents the first report of Meloidogyne hispanica in the USA and North America. Exeley Inc. 2021-12-01 /pmc/articles/PMC8634290/ /pubmed/34881368 http://dx.doi.org/10.21307/jofnem-2021-098 Text en © 2021 Authors https://creativecommons.org/licenses/by/4.0/This is an Open Access article licensed under the Creative Commons CC BY 4.0 license, https://creativecommons.org/licenses/by/4.0/
spellingShingle Life Sciences
Skantar, Andrea M.
Handoo, Zafar A.
Subbotin, Sergei A.
Kantor, Mihail R.
Vieira, Paulo
Agudelo, Paula
Hult, Maria N.
Rogers, Stephen
First report of Seville root-knot nematode, Meloidogyne hispanica (Nematoda: Meloidogynidae) in the USA and North America
title First report of Seville root-knot nematode, Meloidogyne hispanica (Nematoda: Meloidogynidae) in the USA and North America
title_full First report of Seville root-knot nematode, Meloidogyne hispanica (Nematoda: Meloidogynidae) in the USA and North America
title_fullStr First report of Seville root-knot nematode, Meloidogyne hispanica (Nematoda: Meloidogynidae) in the USA and North America
title_full_unstemmed First report of Seville root-knot nematode, Meloidogyne hispanica (Nematoda: Meloidogynidae) in the USA and North America
title_short First report of Seville root-knot nematode, Meloidogyne hispanica (Nematoda: Meloidogynidae) in the USA and North America
title_sort first report of seville root-knot nematode, meloidogyne hispanica (nematoda: meloidogynidae) in the usa and north america
topic Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634290/
https://www.ncbi.nlm.nih.gov/pubmed/34881368
http://dx.doi.org/10.21307/jofnem-2021-098
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