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Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia

Until recently, the stem gall nematode Pterotylenchus cecidogenus was only registered in eastern Colombia. However, the disease has recently been observed in central Colombian oil palm plantations that use Desmodium ovalifolium as a cover crop. Soil, root, stem, and leaf samples were collected from...

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Autores principales: Velandia, Oscar, Mestizo, Yuri, Medina, Héctor Camilo, Riascos-Ortiz, Donald, De Agudelo, Francia Varón, Sarria, Greicy Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Exeley Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634294/
https://www.ncbi.nlm.nih.gov/pubmed/34881369
http://dx.doi.org/10.21307/jofnem-2021-099
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author Velandia, Oscar
Mestizo, Yuri
Medina, Héctor Camilo
Riascos-Ortiz, Donald
De Agudelo, Francia Varón
Sarria, Greicy Andrea
author_facet Velandia, Oscar
Mestizo, Yuri
Medina, Héctor Camilo
Riascos-Ortiz, Donald
De Agudelo, Francia Varón
Sarria, Greicy Andrea
author_sort Velandia, Oscar
collection PubMed
description Until recently, the stem gall nematode Pterotylenchus cecidogenus was only registered in eastern Colombia. However, the disease has recently been observed in central Colombian oil palm plantations that use Desmodium ovalifolium as a cover crop. Soil, root, stem, and leaf samples were collected from D. ovalifolium. Plants showed foliar yellowing, leaf drying, and galls within stem nodes. Nematodes were identified, and the distribution, population density, and relative importance of different genera were determined. We performed morphometric and molecular identification of nematodes associated with gall symptoms. The D2-D3 segment of the large subunit-28S of ribosomal ribonucleic acid (RNA) and internal transcribed spacer (ITS) was sequenced, and phylogenetic analysis was performed. P. cecidogenus mainly occurred in the galls and to a lesser extent in the roots and soil. Nematodes were not found in leaf or inflorescence tissue. Morphological and morphometric data confirm the presence of P. cecidogenus in the stems of D. ovalifolium with gall symptoms. This study is the first to report deoxyribonucleic acid (DNA) sequences of P. cecidogenus. Based on D2-D3 and ITS partial sequences, P. cecidogenus is a sister species of the leaf-galling nematode Ditylenchus phyllobius (Sinm. Orrina phyllobia).
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spelling pubmed-86342942021-12-07 Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia Velandia, Oscar Mestizo, Yuri Medina, Héctor Camilo Riascos-Ortiz, Donald De Agudelo, Francia Varón Sarria, Greicy Andrea J Nematol Life Sciences Until recently, the stem gall nematode Pterotylenchus cecidogenus was only registered in eastern Colombia. However, the disease has recently been observed in central Colombian oil palm plantations that use Desmodium ovalifolium as a cover crop. Soil, root, stem, and leaf samples were collected from D. ovalifolium. Plants showed foliar yellowing, leaf drying, and galls within stem nodes. Nematodes were identified, and the distribution, population density, and relative importance of different genera were determined. We performed morphometric and molecular identification of nematodes associated with gall symptoms. The D2-D3 segment of the large subunit-28S of ribosomal ribonucleic acid (RNA) and internal transcribed spacer (ITS) was sequenced, and phylogenetic analysis was performed. P. cecidogenus mainly occurred in the galls and to a lesser extent in the roots and soil. Nematodes were not found in leaf or inflorescence tissue. Morphological and morphometric data confirm the presence of P. cecidogenus in the stems of D. ovalifolium with gall symptoms. This study is the first to report deoxyribonucleic acid (DNA) sequences of P. cecidogenus. Based on D2-D3 and ITS partial sequences, P. cecidogenus is a sister species of the leaf-galling nematode Ditylenchus phyllobius (Sinm. Orrina phyllobia). Exeley Inc. 2021-12-01 /pmc/articles/PMC8634294/ /pubmed/34881369 http://dx.doi.org/10.21307/jofnem-2021-099 Text en © 2021 Authors https://creativecommons.org/licenses/by/4.0/This is an Open Access article licensed under the Creative Commons CC BY 4.0 license, https://creativecommons.org/licenses/by/4.0/
spellingShingle Life Sciences
Velandia, Oscar
Mestizo, Yuri
Medina, Héctor Camilo
Riascos-Ortiz, Donald
De Agudelo, Francia Varón
Sarria, Greicy Andrea
Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia
title Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia
title_full Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia
title_fullStr Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia
title_full_unstemmed Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia
title_short Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia
title_sort characterization of pterotylenchus cecidogenus in desmodium ovalifolium cover crop from oil palm plantations in central colombia
topic Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634294/
https://www.ncbi.nlm.nih.gov/pubmed/34881369
http://dx.doi.org/10.21307/jofnem-2021-099
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