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Segregating Complete Tf2 Elements Are Largely Neutral in Fission Yeast
Transposable elements (TEs) comprise a large proportion of the eukaryote genomes. Yet it remains poorly understood how TEs influence the fitness of the hosts carrying them. Here, we empirically test the impact of TEs on the host fitness in the fission yeast Schizosaccharomyces pombe. We find that tw...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634392/ https://www.ncbi.nlm.nih.gov/pubmed/34791222 http://dx.doi.org/10.1093/gbe/evab254 |
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author | Wang, Yan Wang, Qin Wu, Zhiwei Han, Guan-Zhu |
author_facet | Wang, Yan Wang, Qin Wu, Zhiwei Han, Guan-Zhu |
author_sort | Wang, Yan |
collection | PubMed |
description | Transposable elements (TEs) comprise a large proportion of the eukaryote genomes. Yet it remains poorly understood how TEs influence the fitness of the hosts carrying them. Here, we empirically test the impact of TEs on the host fitness in the fission yeast Schizosaccharomyces pombe. We find that two families of TEs (Tf1 and Tf2 elements), both of which belong to long terminal repeat retrotransposons, are highly polymorphic among individual S. pombe strains. Only 13 complete Tf2 elements are identified in S. pombe laboratory strain 972. These 13 Tf2 elements integrated into host genomes in very recent time and are segregating within the S. pombe population. Through knocking out each of the 13 Tf2 elements in S. pombe strain 972, we find Tf2 knockout does not affect the host fitness, and Tf2 elements do not alter the expression of nearby genes. Challenged by diverse forms of stress, the Tf2 knockout strains do not exhibit different growth rates from wild-type strain. Together, we conclude that segregating complete Tf2 elements insertions are largely neutral to host fitness in the fission yeast. Our study provides genome-wide empirical support for the selfish nature of TEs in fission yeast. |
format | Online Article Text |
id | pubmed-8634392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-86343922021-12-01 Segregating Complete Tf2 Elements Are Largely Neutral in Fission Yeast Wang, Yan Wang, Qin Wu, Zhiwei Han, Guan-Zhu Genome Biol Evol Research Article Transposable elements (TEs) comprise a large proportion of the eukaryote genomes. Yet it remains poorly understood how TEs influence the fitness of the hosts carrying them. Here, we empirically test the impact of TEs on the host fitness in the fission yeast Schizosaccharomyces pombe. We find that two families of TEs (Tf1 and Tf2 elements), both of which belong to long terminal repeat retrotransposons, are highly polymorphic among individual S. pombe strains. Only 13 complete Tf2 elements are identified in S. pombe laboratory strain 972. These 13 Tf2 elements integrated into host genomes in very recent time and are segregating within the S. pombe population. Through knocking out each of the 13 Tf2 elements in S. pombe strain 972, we find Tf2 knockout does not affect the host fitness, and Tf2 elements do not alter the expression of nearby genes. Challenged by diverse forms of stress, the Tf2 knockout strains do not exhibit different growth rates from wild-type strain. Together, we conclude that segregating complete Tf2 elements insertions are largely neutral to host fitness in the fission yeast. Our study provides genome-wide empirical support for the selfish nature of TEs in fission yeast. Oxford University Press 2021-11-17 /pmc/articles/PMC8634392/ /pubmed/34791222 http://dx.doi.org/10.1093/gbe/evab254 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Wang, Yan Wang, Qin Wu, Zhiwei Han, Guan-Zhu Segregating Complete Tf2 Elements Are Largely Neutral in Fission Yeast |
title | Segregating Complete Tf2 Elements Are Largely Neutral in Fission Yeast |
title_full | Segregating Complete Tf2 Elements Are Largely Neutral in Fission Yeast |
title_fullStr | Segregating Complete Tf2 Elements Are Largely Neutral in Fission Yeast |
title_full_unstemmed | Segregating Complete Tf2 Elements Are Largely Neutral in Fission Yeast |
title_short | Segregating Complete Tf2 Elements Are Largely Neutral in Fission Yeast |
title_sort | segregating complete tf2 elements are largely neutral in fission yeast |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634392/ https://www.ncbi.nlm.nih.gov/pubmed/34791222 http://dx.doi.org/10.1093/gbe/evab254 |
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