Cargando…
A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application
Corynebacterium glutamicum is one of the important industrial microorganisms for production of amino acids and other value-added compounds. Most expression vectors used in C. glutamicum are based on inducible promoter (P( tac ) or P( trc )) activated by isopropyl-β-D-thiogalactopyranoside (IPTG). Ho...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634428/ https://www.ncbi.nlm.nih.gov/pubmed/34869258 http://dx.doi.org/10.3389/fbioe.2021.746322 |
_version_ | 1784608127177457664 |
---|---|
author | Lu, Nan Zhang, Chenglin Zhang, Wenjie Xu, Haoran Li, Yuhong Wei, Minhua Meng, Jing Meng, Yan Wang, Junzhe Chen, Ning |
author_facet | Lu, Nan Zhang, Chenglin Zhang, Wenjie Xu, Haoran Li, Yuhong Wei, Minhua Meng, Jing Meng, Yan Wang, Junzhe Chen, Ning |
author_sort | Lu, Nan |
collection | PubMed |
description | Corynebacterium glutamicum is one of the important industrial microorganisms for production of amino acids and other value-added compounds. Most expression vectors used in C. glutamicum are based on inducible promoter (P( tac ) or P( trc )) activated by isopropyl-β-D-thiogalactopyranoside (IPTG). However, these vectors seem unsuitable for large-scale industrial production due to the high cost and toxicity of IPTG. Myo-inositol is an ideal inducer because of its non-toxicity and lower price. In this study, a myo-inositol-inducible expression vector pMI-4, derived from the expression vector pXMJ19, was constructed. Besides the original chloramphenicol resistance gene cat, multiple cloning sites, and rrnB terminator, the pMI-4 (6,643 bp) contains the iolR ( q ) cassette and the myo-inositol-inducible promoter P( iolT1 ). The pMI-4 could stably replicate in the C. glutamicum host. Meanwhile, the non-myo-inositol degradation host strain C. glutamicumΔiolGΔoxiCΔoxiDΔoxiE for maintaining the pMI-4 was developed. Overexpression of hemA ( M ) and hemL using pMI-4 resulted in a significant accumulation of 5-aminolevulinic acid, indicating its potential application in metabolic engineering and industrial fermentation. |
format | Online Article Text |
id | pubmed-8634428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86344282021-12-02 A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application Lu, Nan Zhang, Chenglin Zhang, Wenjie Xu, Haoran Li, Yuhong Wei, Minhua Meng, Jing Meng, Yan Wang, Junzhe Chen, Ning Front Bioeng Biotechnol Bioengineering and Biotechnology Corynebacterium glutamicum is one of the important industrial microorganisms for production of amino acids and other value-added compounds. Most expression vectors used in C. glutamicum are based on inducible promoter (P( tac ) or P( trc )) activated by isopropyl-β-D-thiogalactopyranoside (IPTG). However, these vectors seem unsuitable for large-scale industrial production due to the high cost and toxicity of IPTG. Myo-inositol is an ideal inducer because of its non-toxicity and lower price. In this study, a myo-inositol-inducible expression vector pMI-4, derived from the expression vector pXMJ19, was constructed. Besides the original chloramphenicol resistance gene cat, multiple cloning sites, and rrnB terminator, the pMI-4 (6,643 bp) contains the iolR ( q ) cassette and the myo-inositol-inducible promoter P( iolT1 ). The pMI-4 could stably replicate in the C. glutamicum host. Meanwhile, the non-myo-inositol degradation host strain C. glutamicumΔiolGΔoxiCΔoxiDΔoxiE for maintaining the pMI-4 was developed. Overexpression of hemA ( M ) and hemL using pMI-4 resulted in a significant accumulation of 5-aminolevulinic acid, indicating its potential application in metabolic engineering and industrial fermentation. Frontiers Media S.A. 2021-11-15 /pmc/articles/PMC8634428/ /pubmed/34869258 http://dx.doi.org/10.3389/fbioe.2021.746322 Text en Copyright © 2021 Lu, Zhang, Zhang, Xu, Li, Wei, Meng, Meng, Wang and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Lu, Nan Zhang, Chenglin Zhang, Wenjie Xu, Haoran Li, Yuhong Wei, Minhua Meng, Jing Meng, Yan Wang, Junzhe Chen, Ning A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application |
title | A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application |
title_full | A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application |
title_fullStr | A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application |
title_full_unstemmed | A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application |
title_short | A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application |
title_sort | myo-inositol-inducible expression system for corynebacterium glutamicum and its application |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634428/ https://www.ncbi.nlm.nih.gov/pubmed/34869258 http://dx.doi.org/10.3389/fbioe.2021.746322 |
work_keys_str_mv | AT lunan amyoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT zhangchenglin amyoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT zhangwenjie amyoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT xuhaoran amyoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT liyuhong amyoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT weiminhua amyoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT mengjing amyoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT mengyan amyoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT wangjunzhe amyoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT chenning amyoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT lunan myoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT zhangchenglin myoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT zhangwenjie myoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT xuhaoran myoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT liyuhong myoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT weiminhua myoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT mengjing myoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT mengyan myoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT wangjunzhe myoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication AT chenning myoinositolinducibleexpressionsystemforcorynebacteriumglutamicumanditsapplication |