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Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, posse...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634467/ https://www.ncbi.nlm.nih.gov/pubmed/34869265 http://dx.doi.org/10.3389/fbioe.2021.757767 |
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author | Li, Youbin Liu, Yuzhe Bai, Haotian Li, Ronghang Shang, Jing Zhu, Zhengqing Zhu, Liwei Zhu, Chenyi Che, Zhenjia Wang, Jincheng Liu, He Huang, Lanfeng |
author_facet | Li, Youbin Liu, Yuzhe Bai, Haotian Li, Ronghang Shang, Jing Zhu, Zhengqing Zhu, Liwei Zhu, Chenyi Che, Zhenjia Wang, Jincheng Liu, He Huang, Lanfeng |
author_sort | Li, Youbin |
collection | PubMed |
description | Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, possess customized structure and strength. The addition of a growth factor coating to the scaffold introduces a specific form of biological activation. Vascular endothelial growth factor (VEGF) is key to angiogenesis and osteogenesis in vivo. We designed a porous titanium alloy scaffold/thermosensitive collagen hydrogel system, equipped with VEGF, to promote local osseointegration and angiogenesis. We also verified the VEGF release via thermosensitive collagen and proliferation and induction of the human umbilical vein endothelial cells (HUVECs) via the composite system in vitro. In vivo, using microscopic computed tomography (Micro-CT), histology, and immunohistochemistry analysis, we confirmed that the composite scaffold aids in angiogenesis-mediated bone regeneration, and promotes significantly more bone integration. We also discovered that the composite scaffold has excellent biocompatibility, provides bioactive VEGF for angiogenesis and osteointegration, and provides an important theoretical basis for the restoration of local blood supply and strengthening of bone integration. |
format | Online Article Text |
id | pubmed-8634467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86344672021-12-02 Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants Li, Youbin Liu, Yuzhe Bai, Haotian Li, Ronghang Shang, Jing Zhu, Zhengqing Zhu, Liwei Zhu, Chenyi Che, Zhenjia Wang, Jincheng Liu, He Huang, Lanfeng Front Bioeng Biotechnol Bioengineering and Biotechnology Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, possess customized structure and strength. The addition of a growth factor coating to the scaffold introduces a specific form of biological activation. Vascular endothelial growth factor (VEGF) is key to angiogenesis and osteogenesis in vivo. We designed a porous titanium alloy scaffold/thermosensitive collagen hydrogel system, equipped with VEGF, to promote local osseointegration and angiogenesis. We also verified the VEGF release via thermosensitive collagen and proliferation and induction of the human umbilical vein endothelial cells (HUVECs) via the composite system in vitro. In vivo, using microscopic computed tomography (Micro-CT), histology, and immunohistochemistry analysis, we confirmed that the composite scaffold aids in angiogenesis-mediated bone regeneration, and promotes significantly more bone integration. We also discovered that the composite scaffold has excellent biocompatibility, provides bioactive VEGF for angiogenesis and osteointegration, and provides an important theoretical basis for the restoration of local blood supply and strengthening of bone integration. Frontiers Media S.A. 2021-11-15 /pmc/articles/PMC8634467/ /pubmed/34869265 http://dx.doi.org/10.3389/fbioe.2021.757767 Text en Copyright © 2021 Li, Liu, Bai, Li, Shang, Zhu, Zhu, Zhu, Che, Wang, Liu and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Li, Youbin Liu, Yuzhe Bai, Haotian Li, Ronghang Shang, Jing Zhu, Zhengqing Zhu, Liwei Zhu, Chenyi Che, Zhenjia Wang, Jincheng Liu, He Huang, Lanfeng Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants |
title | Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants |
title_full | Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants |
title_fullStr | Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants |
title_full_unstemmed | Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants |
title_short | Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants |
title_sort | sustained release of vegf to promote angiogenesis and osteointegration of three-dimensional printed biomimetic titanium alloy implants |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634467/ https://www.ncbi.nlm.nih.gov/pubmed/34869265 http://dx.doi.org/10.3389/fbioe.2021.757767 |
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