Cargando…

Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants

Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, posse...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Youbin, Liu, Yuzhe, Bai, Haotian, Li, Ronghang, Shang, Jing, Zhu, Zhengqing, Zhu, Liwei, Zhu, Chenyi, Che, Zhenjia, Wang, Jincheng, Liu, He, Huang, Lanfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634467/
https://www.ncbi.nlm.nih.gov/pubmed/34869265
http://dx.doi.org/10.3389/fbioe.2021.757767
_version_ 1784608134631784448
author Li, Youbin
Liu, Yuzhe
Bai, Haotian
Li, Ronghang
Shang, Jing
Zhu, Zhengqing
Zhu, Liwei
Zhu, Chenyi
Che, Zhenjia
Wang, Jincheng
Liu, He
Huang, Lanfeng
author_facet Li, Youbin
Liu, Yuzhe
Bai, Haotian
Li, Ronghang
Shang, Jing
Zhu, Zhengqing
Zhu, Liwei
Zhu, Chenyi
Che, Zhenjia
Wang, Jincheng
Liu, He
Huang, Lanfeng
author_sort Li, Youbin
collection PubMed
description Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, possess customized structure and strength. The addition of a growth factor coating to the scaffold introduces a specific form of biological activation. Vascular endothelial growth factor (VEGF) is key to angiogenesis and osteogenesis in vivo. We designed a porous titanium alloy scaffold/thermosensitive collagen hydrogel system, equipped with VEGF, to promote local osseointegration and angiogenesis. We also verified the VEGF release via thermosensitive collagen and proliferation and induction of the human umbilical vein endothelial cells (HUVECs) via the composite system in vitro. In vivo, using microscopic computed tomography (Micro-CT), histology, and immunohistochemistry analysis, we confirmed that the composite scaffold aids in angiogenesis-mediated bone regeneration, and promotes significantly more bone integration. We also discovered that the composite scaffold has excellent biocompatibility, provides bioactive VEGF for angiogenesis and osteointegration, and provides an important theoretical basis for the restoration of local blood supply and strengthening of bone integration.
format Online
Article
Text
id pubmed-8634467
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86344672021-12-02 Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants Li, Youbin Liu, Yuzhe Bai, Haotian Li, Ronghang Shang, Jing Zhu, Zhengqing Zhu, Liwei Zhu, Chenyi Che, Zhenjia Wang, Jincheng Liu, He Huang, Lanfeng Front Bioeng Biotechnol Bioengineering and Biotechnology Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, possess customized structure and strength. The addition of a growth factor coating to the scaffold introduces a specific form of biological activation. Vascular endothelial growth factor (VEGF) is key to angiogenesis and osteogenesis in vivo. We designed a porous titanium alloy scaffold/thermosensitive collagen hydrogel system, equipped with VEGF, to promote local osseointegration and angiogenesis. We also verified the VEGF release via thermosensitive collagen and proliferation and induction of the human umbilical vein endothelial cells (HUVECs) via the composite system in vitro. In vivo, using microscopic computed tomography (Micro-CT), histology, and immunohistochemistry analysis, we confirmed that the composite scaffold aids in angiogenesis-mediated bone regeneration, and promotes significantly more bone integration. We also discovered that the composite scaffold has excellent biocompatibility, provides bioactive VEGF for angiogenesis and osteointegration, and provides an important theoretical basis for the restoration of local blood supply and strengthening of bone integration. Frontiers Media S.A. 2021-11-15 /pmc/articles/PMC8634467/ /pubmed/34869265 http://dx.doi.org/10.3389/fbioe.2021.757767 Text en Copyright © 2021 Li, Liu, Bai, Li, Shang, Zhu, Zhu, Zhu, Che, Wang, Liu and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Li, Youbin
Liu, Yuzhe
Bai, Haotian
Li, Ronghang
Shang, Jing
Zhu, Zhengqing
Zhu, Liwei
Zhu, Chenyi
Che, Zhenjia
Wang, Jincheng
Liu, He
Huang, Lanfeng
Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title_full Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title_fullStr Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title_full_unstemmed Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title_short Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title_sort sustained release of vegf to promote angiogenesis and osteointegration of three-dimensional printed biomimetic titanium alloy implants
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634467/
https://www.ncbi.nlm.nih.gov/pubmed/34869265
http://dx.doi.org/10.3389/fbioe.2021.757767
work_keys_str_mv AT liyoubin sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT liuyuzhe sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT baihaotian sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT lironghang sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT shangjing sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT zhuzhengqing sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT zhuliwei sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT zhuchenyi sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT chezhenjia sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT wangjincheng sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT liuhe sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants
AT huanglanfeng sustainedreleaseofvegftopromoteangiogenesisandosteointegrationofthreedimensionalprintedbiomimetictitaniumalloyimplants