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Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster

Automatic quantification of image parameters is a powerful and necessary tool to explore and analyze crucial cell biological processes. This article describes two ImageJ/Fiji automated macros to approach the analysis of synaptic autophagy and exosome release from 2D confocal images. Emerging studies...

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Autores principales: Sanchez-Mirasierra, Irene, Hernandez-Diaz, Sergio, Ghimire, Saurav, Montecinos-Oliva, Carla, Soukup, Sandra-Fausia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634598/
https://www.ncbi.nlm.nih.gov/pubmed/34869373
http://dx.doi.org/10.3389/fcell.2021.773861
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author Sanchez-Mirasierra, Irene
Hernandez-Diaz, Sergio
Ghimire, Saurav
Montecinos-Oliva, Carla
Soukup, Sandra-Fausia
author_facet Sanchez-Mirasierra, Irene
Hernandez-Diaz, Sergio
Ghimire, Saurav
Montecinos-Oliva, Carla
Soukup, Sandra-Fausia
author_sort Sanchez-Mirasierra, Irene
collection PubMed
description Automatic quantification of image parameters is a powerful and necessary tool to explore and analyze crucial cell biological processes. This article describes two ImageJ/Fiji automated macros to approach the analysis of synaptic autophagy and exosome release from 2D confocal images. Emerging studies point out that exosome biogenesis and autophagy share molecular and organelle components. Indeed, the crosstalk between these two processes may be relevant for brain physiology, neuronal development, and the onset/progression of neurodegenerative disorders. In this context, we describe here the macros “Autophagoquant” and “Exoquant” to assess the quantification of autophagosomes and exosomes at the neuronal presynapse of the Neuromuscular Junction (NMJ) in Drosophila melanogaster using confocal microscopy images. The Drosophila NMJ is a valuable model for the study of synapse biology, autophagy, and exosome release. By use of Autophagoquant and Exoquant, researchers can have an unbiased, standardized, and rapid tool to analyze autophagy and exosomal release in Drosophila NMJ. Code available at: https://github.com/IreneSaMi/Exoquant-Autophagoquant
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spelling pubmed-86345982021-12-02 Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster Sanchez-Mirasierra, Irene Hernandez-Diaz, Sergio Ghimire, Saurav Montecinos-Oliva, Carla Soukup, Sandra-Fausia Front Cell Dev Biol Cell and Developmental Biology Automatic quantification of image parameters is a powerful and necessary tool to explore and analyze crucial cell biological processes. This article describes two ImageJ/Fiji automated macros to approach the analysis of synaptic autophagy and exosome release from 2D confocal images. Emerging studies point out that exosome biogenesis and autophagy share molecular and organelle components. Indeed, the crosstalk between these two processes may be relevant for brain physiology, neuronal development, and the onset/progression of neurodegenerative disorders. In this context, we describe here the macros “Autophagoquant” and “Exoquant” to assess the quantification of autophagosomes and exosomes at the neuronal presynapse of the Neuromuscular Junction (NMJ) in Drosophila melanogaster using confocal microscopy images. The Drosophila NMJ is a valuable model for the study of synapse biology, autophagy, and exosome release. By use of Autophagoquant and Exoquant, researchers can have an unbiased, standardized, and rapid tool to analyze autophagy and exosomal release in Drosophila NMJ. Code available at: https://github.com/IreneSaMi/Exoquant-Autophagoquant Frontiers Media S.A. 2021-11-15 /pmc/articles/PMC8634598/ /pubmed/34869373 http://dx.doi.org/10.3389/fcell.2021.773861 Text en Copyright © 2021 Sanchez-Mirasierra, Hernandez-Diaz, Ghimire, Montecinos-Oliva and Soukup. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Sanchez-Mirasierra, Irene
Hernandez-Diaz, Sergio
Ghimire, Saurav
Montecinos-Oliva, Carla
Soukup, Sandra-Fausia
Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title_full Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title_fullStr Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title_full_unstemmed Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title_short Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title_sort macros to quantify exosome release and autophagy at the neuromuscular junction of drosophila melanogaster
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634598/
https://www.ncbi.nlm.nih.gov/pubmed/34869373
http://dx.doi.org/10.3389/fcell.2021.773861
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