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A Novel Pyroptosis-related Prognostic Model for Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) is the second most lethal malignant tumor because of its significant heterogeneity and complicated molecular pathogenesis. Novel prognostic biomarkers are urgently needed because no effective and reliable prognostic biomarkers currently exist for HCC patients. Increasi...

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Autores principales: Wu, Qianqian, Jiang, Sutian, Cheng, Tong, Xu, Manyu, Lu, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634647/
https://www.ncbi.nlm.nih.gov/pubmed/34869364
http://dx.doi.org/10.3389/fcell.2021.770301
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author Wu, Qianqian
Jiang, Sutian
Cheng, Tong
Xu, Manyu
Lu, Bing
author_facet Wu, Qianqian
Jiang, Sutian
Cheng, Tong
Xu, Manyu
Lu, Bing
author_sort Wu, Qianqian
collection PubMed
description Hepatocellular carcinoma (HCC) is the second most lethal malignant tumor because of its significant heterogeneity and complicated molecular pathogenesis. Novel prognostic biomarkers are urgently needed because no effective and reliable prognostic biomarkers currently exist for HCC patients. Increasing evidence has revealed that pyroptosis plays a role in the occurrence and progression of malignant tumors. However, the relationship between pyroptosis-related genes (PRGs) and HCC patient prognosis remains unclear. In this study, 57 PRGs were obtained from previous studies and GeneCards. The gene expression profiles and clinical data of HCC patients were acquired from public data portals. Least absolute shrinkage and selection operator (LASSO) Cox regression analysis was performed to establish a risk model using TCGA data. Additionally, the risk model was further validated in an independent ICGC dataset. Our results showed that 39 PRGs were significantly differentially expressed between tumor and normal liver tissues in the TCGA cohort. Functional analysis confirmed that these PRGs were enriched in pyroptosis-related pathways. According to univariate Cox regression analysis, 14 differentially expressed PRGs were correlated with the prognosis of HCC patients in the TCGA cohort. A risk model integrating two PRGs was constructed to classify the patients into different risk groups. Poor overall survival was observed in the high-risk group of both TCGA (p < 0.001) and ICGC (p < 0.001) patients. Receiver operating characteristic curves demonstrated the accuracy of the model. Furthermore, the risk score was confirmed as an independent prognostic indicator via multivariate Cox regression analysis (TCGA cohort: HR = 3.346, p < 0.001; ICGC cohort: HR = 3.699, p < 0.001). Moreover, the single-sample gene set enrichment analysis revealed different immune statuses between high- and low-risk groups. In conclusion, our new pyroptosis-related risk model has potential application in predicting the prognosis of HCC patients.
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spelling pubmed-86346472021-12-02 A Novel Pyroptosis-related Prognostic Model for Hepatocellular Carcinoma Wu, Qianqian Jiang, Sutian Cheng, Tong Xu, Manyu Lu, Bing Front Cell Dev Biol Cell and Developmental Biology Hepatocellular carcinoma (HCC) is the second most lethal malignant tumor because of its significant heterogeneity and complicated molecular pathogenesis. Novel prognostic biomarkers are urgently needed because no effective and reliable prognostic biomarkers currently exist for HCC patients. Increasing evidence has revealed that pyroptosis plays a role in the occurrence and progression of malignant tumors. However, the relationship between pyroptosis-related genes (PRGs) and HCC patient prognosis remains unclear. In this study, 57 PRGs were obtained from previous studies and GeneCards. The gene expression profiles and clinical data of HCC patients were acquired from public data portals. Least absolute shrinkage and selection operator (LASSO) Cox regression analysis was performed to establish a risk model using TCGA data. Additionally, the risk model was further validated in an independent ICGC dataset. Our results showed that 39 PRGs were significantly differentially expressed between tumor and normal liver tissues in the TCGA cohort. Functional analysis confirmed that these PRGs were enriched in pyroptosis-related pathways. According to univariate Cox regression analysis, 14 differentially expressed PRGs were correlated with the prognosis of HCC patients in the TCGA cohort. A risk model integrating two PRGs was constructed to classify the patients into different risk groups. Poor overall survival was observed in the high-risk group of both TCGA (p < 0.001) and ICGC (p < 0.001) patients. Receiver operating characteristic curves demonstrated the accuracy of the model. Furthermore, the risk score was confirmed as an independent prognostic indicator via multivariate Cox regression analysis (TCGA cohort: HR = 3.346, p < 0.001; ICGC cohort: HR = 3.699, p < 0.001). Moreover, the single-sample gene set enrichment analysis revealed different immune statuses between high- and low-risk groups. In conclusion, our new pyroptosis-related risk model has potential application in predicting the prognosis of HCC patients. Frontiers Media S.A. 2021-11-15 /pmc/articles/PMC8634647/ /pubmed/34869364 http://dx.doi.org/10.3389/fcell.2021.770301 Text en Copyright © 2021 Wu, Jiang, Cheng, Xu and Lu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Wu, Qianqian
Jiang, Sutian
Cheng, Tong
Xu, Manyu
Lu, Bing
A Novel Pyroptosis-related Prognostic Model for Hepatocellular Carcinoma
title A Novel Pyroptosis-related Prognostic Model for Hepatocellular Carcinoma
title_full A Novel Pyroptosis-related Prognostic Model for Hepatocellular Carcinoma
title_fullStr A Novel Pyroptosis-related Prognostic Model for Hepatocellular Carcinoma
title_full_unstemmed A Novel Pyroptosis-related Prognostic Model for Hepatocellular Carcinoma
title_short A Novel Pyroptosis-related Prognostic Model for Hepatocellular Carcinoma
title_sort novel pyroptosis-related prognostic model for hepatocellular carcinoma
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634647/
https://www.ncbi.nlm.nih.gov/pubmed/34869364
http://dx.doi.org/10.3389/fcell.2021.770301
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