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Diminished amplification of SARS-CoV-2 ORF1ab in a commercial dual-target qRT-PCR diagnostic assay

Here we describe a SARS-CoV-2 variant with diminished amplification of the ORF ORF1ab target in the Cobas® dual-target SARS-CoV-2 assay resulting in a discrepancy of Ct-values (Ct-value 20.7 for the E-gene and Ct-value 30.2 for ORF1ab). Five unique nucleotide mutations were identified in ORF1ab: C11...

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Detalles Bibliográficos
Autores principales:	Popping, Stephanie, Molenkamp, Richard, Weigel, Joachim D., Mutsaers, Pim G.N.J., Voermans, Jolanda C., van Boheemen, Sander, Shamier, Marc C., Sikkema, Reina S., Nieuwenhuijse, David F., Oude Munnink, Bas B., Koopmans, Marion P.G., van Kampen, Jeroen J.A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Published by Elsevier B.V. 2022
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634734/ https://www.ncbi.nlm.nih.gov/pubmed/34863783 http://dx.doi.org/10.1016/j.jviromet.2021.114397

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