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Identification and Analysis of Zinc Efficiency-Associated Loci in Maize
Zinc (Zn) deficiency, a globally predominant micronutrient disorder in crops and humans, reduces crop yields and adversely impacts human health. Despite numerous studies on the physiological mechanisms underlying Zn deficiency tolerance, its genetic basis of molecular mechanism is still poorly under...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634756/ https://www.ncbi.nlm.nih.gov/pubmed/34868123 http://dx.doi.org/10.3389/fpls.2021.739282 |
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author | Xu, Jianqin Wang, Xuejie Zhu, Huaqing Yu, Futong |
author_facet | Xu, Jianqin Wang, Xuejie Zhu, Huaqing Yu, Futong |
author_sort | Xu, Jianqin |
collection | PubMed |
description | Zinc (Zn) deficiency, a globally predominant micronutrient disorder in crops and humans, reduces crop yields and adversely impacts human health. Despite numerous studies on the physiological mechanisms underlying Zn deficiency tolerance, its genetic basis of molecular mechanism is still poorly understood. Thus, the Zn efficiency of 20 maize inbred lines was evaluated, and a quantitative trait locus (QTL) analysis was performed in the recombination inbred line population derived from the most Zn-efficient (Ye478) and Zn-inefficient inbred line (Wu312) to identify the candidate genes associated with Zn deficiency tolerance. On this basis, we analyzed the expression of ZmZIP1-ZmZIP8. Thirteen QTLs for the traits associated with Zn deficiency tolerance were detected, explaining 7.6–63.5% of the phenotypic variation. The genes responsible for Zn uptake and transport across membranes (ZmZIP3, ZmHMA3, ZmHMA4) were identified, which probably form a sophisticated network to regulate the uptake, translocation, and redistribution of Zn. Additionally, we identified the genes involved in the indole-3-acetic acid (IAA) biosynthesis (ZmIGPS) and auxin-dependent gene regulation (ZmIAA). Notably, a high upregulation of ZmZIP3 was found in the Zn-deficient root of Ye478, but not in that of Wu312. Additionally, ZmZIP4, ZmZIP5, and ZmZIP7 were up-regulated in the Zn-deficient roots of Ye478 and Wu312. Our findings provide a new insight into the genetic basis of Zn deficiency tolerance. |
format | Online Article Text |
id | pubmed-8634756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86347562021-12-02 Identification and Analysis of Zinc Efficiency-Associated Loci in Maize Xu, Jianqin Wang, Xuejie Zhu, Huaqing Yu, Futong Front Plant Sci Plant Science Zinc (Zn) deficiency, a globally predominant micronutrient disorder in crops and humans, reduces crop yields and adversely impacts human health. Despite numerous studies on the physiological mechanisms underlying Zn deficiency tolerance, its genetic basis of molecular mechanism is still poorly understood. Thus, the Zn efficiency of 20 maize inbred lines was evaluated, and a quantitative trait locus (QTL) analysis was performed in the recombination inbred line population derived from the most Zn-efficient (Ye478) and Zn-inefficient inbred line (Wu312) to identify the candidate genes associated with Zn deficiency tolerance. On this basis, we analyzed the expression of ZmZIP1-ZmZIP8. Thirteen QTLs for the traits associated with Zn deficiency tolerance were detected, explaining 7.6–63.5% of the phenotypic variation. The genes responsible for Zn uptake and transport across membranes (ZmZIP3, ZmHMA3, ZmHMA4) were identified, which probably form a sophisticated network to regulate the uptake, translocation, and redistribution of Zn. Additionally, we identified the genes involved in the indole-3-acetic acid (IAA) biosynthesis (ZmIGPS) and auxin-dependent gene regulation (ZmIAA). Notably, a high upregulation of ZmZIP3 was found in the Zn-deficient root of Ye478, but not in that of Wu312. Additionally, ZmZIP4, ZmZIP5, and ZmZIP7 were up-regulated in the Zn-deficient roots of Ye478 and Wu312. Our findings provide a new insight into the genetic basis of Zn deficiency tolerance. Frontiers Media S.A. 2021-11-15 /pmc/articles/PMC8634756/ /pubmed/34868123 http://dx.doi.org/10.3389/fpls.2021.739282 Text en Copyright © 2021 Xu, Wang, Zhu and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Xu, Jianqin Wang, Xuejie Zhu, Huaqing Yu, Futong Identification and Analysis of Zinc Efficiency-Associated Loci in Maize |
title | Identification and Analysis of Zinc Efficiency-Associated Loci in Maize |
title_full | Identification and Analysis of Zinc Efficiency-Associated Loci in Maize |
title_fullStr | Identification and Analysis of Zinc Efficiency-Associated Loci in Maize |
title_full_unstemmed | Identification and Analysis of Zinc Efficiency-Associated Loci in Maize |
title_short | Identification and Analysis of Zinc Efficiency-Associated Loci in Maize |
title_sort | identification and analysis of zinc efficiency-associated loci in maize |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634756/ https://www.ncbi.nlm.nih.gov/pubmed/34868123 http://dx.doi.org/10.3389/fpls.2021.739282 |
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