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CircCRIM1 Promotes Hepatocellular Carcinoma Proliferation and Angiogenesis by Sponging miR-378a-3p and Regulating SKP2 Expression

Mounting evidence has demonstrated that circular RNAs have an important function in tumorigenesis and cancer evolvement. CircCRIM1 has been shown to be a poor prognostic element in multiple human malignancies. However, the clinical significance and mechanism of circCRIM1 in hepatocellular carcinoma...

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Autores principales: Ji, Yang, Yang, Shikun, Yan, Xueqi, Zhu, Li, Yang, Wenjie, Yang, Xinchen, Yu, Fei, Shi, Longqing, Zhu, Xi, Lu, Yunjie, Zhang, Chuanyong, Lu, Hao, Zhang, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634842/
https://www.ncbi.nlm.nih.gov/pubmed/34869393
http://dx.doi.org/10.3389/fcell.2021.796686
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author Ji, Yang
Yang, Shikun
Yan, Xueqi
Zhu, Li
Yang, Wenjie
Yang, Xinchen
Yu, Fei
Shi, Longqing
Zhu, Xi
Lu, Yunjie
Zhang, Chuanyong
Lu, Hao
Zhang, Feng
author_facet Ji, Yang
Yang, Shikun
Yan, Xueqi
Zhu, Li
Yang, Wenjie
Yang, Xinchen
Yu, Fei
Shi, Longqing
Zhu, Xi
Lu, Yunjie
Zhang, Chuanyong
Lu, Hao
Zhang, Feng
author_sort Ji, Yang
collection PubMed
description Mounting evidence has demonstrated that circular RNAs have an important function in tumorigenesis and cancer evolvement. CircCRIM1 has been shown to be a poor prognostic element in multiple human malignancies. However, the clinical significance and mechanism of circCRIM1 in hepatocellular carcinoma (HCC) is still unclear. The present study confirmed the expression level of circCRIM1 using quantitative real-time PCR. In addition, circCRIM1 siRNA and overexpression vectors were used for transfection into LM3 or Huh7 cells to down- or up-regulate the expression of circCRIM1. In vitro and in vivo experiments were performed to explore the function of circCRIM1 in HCC. RNA pull-down, RNA immunoprecipitation, fluorescent in situ hybridization, and luciferase reporter assays were conducted to confirm the relationship between miR-378a-3p and circCRIM1 or S-phase kinase-associated protein 2 (SKP2) in HCC. Then, circCRIM1 was up-regulated in HCC and its expression level was significantly associated with poor prognosis and clinicopathologic characteristics. CircCRIM1 enhanced the proliferation and angiogenesis of HCC cells in vitro and promoted xenograft growth in vivo. Moreover, circCRIM1 upregulated the expression of SKP2 by functioning as a sponge for miR-378a-3p. These findings suggest that circCRIM1 boosts the HCC progression via the miR-378-3p/SKP2 axis and may act as a crucial epigenetic therapeutic molecule target in HCC.
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spelling pubmed-86348422021-12-02 CircCRIM1 Promotes Hepatocellular Carcinoma Proliferation and Angiogenesis by Sponging miR-378a-3p and Regulating SKP2 Expression Ji, Yang Yang, Shikun Yan, Xueqi Zhu, Li Yang, Wenjie Yang, Xinchen Yu, Fei Shi, Longqing Zhu, Xi Lu, Yunjie Zhang, Chuanyong Lu, Hao Zhang, Feng Front Cell Dev Biol Cell and Developmental Biology Mounting evidence has demonstrated that circular RNAs have an important function in tumorigenesis and cancer evolvement. CircCRIM1 has been shown to be a poor prognostic element in multiple human malignancies. However, the clinical significance and mechanism of circCRIM1 in hepatocellular carcinoma (HCC) is still unclear. The present study confirmed the expression level of circCRIM1 using quantitative real-time PCR. In addition, circCRIM1 siRNA and overexpression vectors were used for transfection into LM3 or Huh7 cells to down- or up-regulate the expression of circCRIM1. In vitro and in vivo experiments were performed to explore the function of circCRIM1 in HCC. RNA pull-down, RNA immunoprecipitation, fluorescent in situ hybridization, and luciferase reporter assays were conducted to confirm the relationship between miR-378a-3p and circCRIM1 or S-phase kinase-associated protein 2 (SKP2) in HCC. Then, circCRIM1 was up-regulated in HCC and its expression level was significantly associated with poor prognosis and clinicopathologic characteristics. CircCRIM1 enhanced the proliferation and angiogenesis of HCC cells in vitro and promoted xenograft growth in vivo. Moreover, circCRIM1 upregulated the expression of SKP2 by functioning as a sponge for miR-378a-3p. These findings suggest that circCRIM1 boosts the HCC progression via the miR-378-3p/SKP2 axis and may act as a crucial epigenetic therapeutic molecule target in HCC. Frontiers Media S.A. 2021-11-12 /pmc/articles/PMC8634842/ /pubmed/34869393 http://dx.doi.org/10.3389/fcell.2021.796686 Text en Copyright © 2021 Ji, Yang, Yan, Zhu, Yang, Yang, Yu, Shi, Zhu, Lu, Zhang, Lu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Ji, Yang
Yang, Shikun
Yan, Xueqi
Zhu, Li
Yang, Wenjie
Yang, Xinchen
Yu, Fei
Shi, Longqing
Zhu, Xi
Lu, Yunjie
Zhang, Chuanyong
Lu, Hao
Zhang, Feng
CircCRIM1 Promotes Hepatocellular Carcinoma Proliferation and Angiogenesis by Sponging miR-378a-3p and Regulating SKP2 Expression
title CircCRIM1 Promotes Hepatocellular Carcinoma Proliferation and Angiogenesis by Sponging miR-378a-3p and Regulating SKP2 Expression
title_full CircCRIM1 Promotes Hepatocellular Carcinoma Proliferation and Angiogenesis by Sponging miR-378a-3p and Regulating SKP2 Expression
title_fullStr CircCRIM1 Promotes Hepatocellular Carcinoma Proliferation and Angiogenesis by Sponging miR-378a-3p and Regulating SKP2 Expression
title_full_unstemmed CircCRIM1 Promotes Hepatocellular Carcinoma Proliferation and Angiogenesis by Sponging miR-378a-3p and Regulating SKP2 Expression
title_short CircCRIM1 Promotes Hepatocellular Carcinoma Proliferation and Angiogenesis by Sponging miR-378a-3p and Regulating SKP2 Expression
title_sort circcrim1 promotes hepatocellular carcinoma proliferation and angiogenesis by sponging mir-378a-3p and regulating skp2 expression
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634842/
https://www.ncbi.nlm.nih.gov/pubmed/34869393
http://dx.doi.org/10.3389/fcell.2021.796686
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