Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging

Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improv...

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Detalles Bibliográficos
Autores principales: Hennig, Simon, Manstein, Dietmar J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634860/
https://www.ncbi.nlm.nih.gov/pubmed/34228908
http://dx.doi.org/10.1002/2211-5463.13246
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author Hennig, Simon
Manstein, Dietmar J.
author_facet Hennig, Simon
Manstein, Dietmar J.
author_sort Hennig, Simon
collection PubMed
description Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3‐fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state‐of‐the‐art commercial laser scanning microscope systems.
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spelling pubmed-86348602021-12-08 Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging Hennig, Simon Manstein, Dietmar J. FEBS Open Bio Method Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3‐fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state‐of‐the‐art commercial laser scanning microscope systems. John Wiley and Sons Inc. 2021-07-26 /pmc/articles/PMC8634860/ /pubmed/34228908 http://dx.doi.org/10.1002/2211-5463.13246 Text en © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Hennig, Simon
Manstein, Dietmar J.
Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title_full Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title_fullStr Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title_full_unstemmed Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title_short Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title_sort improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634860/
https://www.ncbi.nlm.nih.gov/pubmed/34228908
http://dx.doi.org/10.1002/2211-5463.13246
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AT mansteindietmarj improvementofimageresolutionbycombiningenhancedconfocalmicroscopyandquantumdottriexcitonimaging

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