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Epidemiological Analysis From 2018 to 2020 in China and Prevention Strategy of Porcine Circovirus Type 2

Porcine circovirus type 2 (PCV2) is one of the smallest known animal viruses and is the main pathogen of PCV-associated diseases (PCVAD). Epidemiological surveillance results have shown that the PCV2 infection rate is on the rise in China, thus, PCV2 disease prevention and control has become a huge...

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Autores principales: Huang, Ying, Chen, Xianghong, Long, Yunzhi, Yang, Liu, Song, Wenbo, Liu, Jinjin, Li, Qianqian, Liang, Gong, Yu, Daobing, Huang, Chao, Tang, Xibiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635018/
https://www.ncbi.nlm.nih.gov/pubmed/34869727
http://dx.doi.org/10.3389/fvets.2021.753297
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author Huang, Ying
Chen, Xianghong
Long, Yunzhi
Yang, Liu
Song, Wenbo
Liu, Jinjin
Li, Qianqian
Liang, Gong
Yu, Daobing
Huang, Chao
Tang, Xibiao
author_facet Huang, Ying
Chen, Xianghong
Long, Yunzhi
Yang, Liu
Song, Wenbo
Liu, Jinjin
Li, Qianqian
Liang, Gong
Yu, Daobing
Huang, Chao
Tang, Xibiao
author_sort Huang, Ying
collection PubMed
description Porcine circovirus type 2 (PCV2) is one of the smallest known animal viruses and is the main pathogen of PCV-associated diseases (PCVAD). Epidemiological surveillance results have shown that the PCV2 infection rate is on the rise in China, thus, PCV2 disease prevention and control has become a huge challenge for the Chinese swine industry. We collected clinical samples from multiple different provinces in China from 2018 to 2020 and found that the positive rate of PCV2 was 53% (3619/6872), identity between the cloned 62 ORF2 genes was 84.4–100% and identity between the cloned 62 ORF2 sequences and reference sequence was 72.9–99.8%. Genetic evolution analysis found that PCV2d accounted for 79% (49/62 samples), PCV2a for 12.9% (8/62 samples), PCV2b for 8% (5/62 samples), and PCV2c and PCV2e genotypes were not found. However, most commercial PCV2 subunit vaccines are based on the PCV2a genotype, and there are very few vaccines based on PCV2b or PCV2d. Therefore, the homologous and heterologous protection ability of PCV2b and PCV2d Cap proteins based on the baculovirus against the PCV2b and PCV2d infections was evaluated, which is expected to design and develop excellent PCV2 protein vaccine candidates. This study found that both PCV2b and PCV2d Cap proteins can increase the level of humoral immunity and cellular immune response in mice. Importantly, both PCV2b and PCV2d cap proteins can provide homologous and heterologous protection against the PCV2b and PCV2d viruses. Overall, this study provides a reference for the prevention and control of PCVAD in mainland China and the development of PCV2 vaccines.
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spelling pubmed-86350182021-12-02 Epidemiological Analysis From 2018 to 2020 in China and Prevention Strategy of Porcine Circovirus Type 2 Huang, Ying Chen, Xianghong Long, Yunzhi Yang, Liu Song, Wenbo Liu, Jinjin Li, Qianqian Liang, Gong Yu, Daobing Huang, Chao Tang, Xibiao Front Vet Sci Veterinary Science Porcine circovirus type 2 (PCV2) is one of the smallest known animal viruses and is the main pathogen of PCV-associated diseases (PCVAD). Epidemiological surveillance results have shown that the PCV2 infection rate is on the rise in China, thus, PCV2 disease prevention and control has become a huge challenge for the Chinese swine industry. We collected clinical samples from multiple different provinces in China from 2018 to 2020 and found that the positive rate of PCV2 was 53% (3619/6872), identity between the cloned 62 ORF2 genes was 84.4–100% and identity between the cloned 62 ORF2 sequences and reference sequence was 72.9–99.8%. Genetic evolution analysis found that PCV2d accounted for 79% (49/62 samples), PCV2a for 12.9% (8/62 samples), PCV2b for 8% (5/62 samples), and PCV2c and PCV2e genotypes were not found. However, most commercial PCV2 subunit vaccines are based on the PCV2a genotype, and there are very few vaccines based on PCV2b or PCV2d. Therefore, the homologous and heterologous protection ability of PCV2b and PCV2d Cap proteins based on the baculovirus against the PCV2b and PCV2d infections was evaluated, which is expected to design and develop excellent PCV2 protein vaccine candidates. This study found that both PCV2b and PCV2d Cap proteins can increase the level of humoral immunity and cellular immune response in mice. Importantly, both PCV2b and PCV2d cap proteins can provide homologous and heterologous protection against the PCV2b and PCV2d viruses. Overall, this study provides a reference for the prevention and control of PCVAD in mainland China and the development of PCV2 vaccines. Frontiers Media S.A. 2021-11-16 /pmc/articles/PMC8635018/ /pubmed/34869727 http://dx.doi.org/10.3389/fvets.2021.753297 Text en Copyright © 2021 Huang, Chen, Long, Yang, Song, Liu, Li, Liang, Yu, Huang and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Huang, Ying
Chen, Xianghong
Long, Yunzhi
Yang, Liu
Song, Wenbo
Liu, Jinjin
Li, Qianqian
Liang, Gong
Yu, Daobing
Huang, Chao
Tang, Xibiao
Epidemiological Analysis From 2018 to 2020 in China and Prevention Strategy of Porcine Circovirus Type 2
title Epidemiological Analysis From 2018 to 2020 in China and Prevention Strategy of Porcine Circovirus Type 2
title_full Epidemiological Analysis From 2018 to 2020 in China and Prevention Strategy of Porcine Circovirus Type 2
title_fullStr Epidemiological Analysis From 2018 to 2020 in China and Prevention Strategy of Porcine Circovirus Type 2
title_full_unstemmed Epidemiological Analysis From 2018 to 2020 in China and Prevention Strategy of Porcine Circovirus Type 2
title_short Epidemiological Analysis From 2018 to 2020 in China and Prevention Strategy of Porcine Circovirus Type 2
title_sort epidemiological analysis from 2018 to 2020 in china and prevention strategy of porcine circovirus type 2
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635018/
https://www.ncbi.nlm.nih.gov/pubmed/34869727
http://dx.doi.org/10.3389/fvets.2021.753297
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