Cargando…

Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo

Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome...

Descripción completa

Detalles Bibliográficos
Autores principales: Rasha, Elsayim, Alkhulaifi, Manal M., AlOthman, Monerah, Khalid, Ibrahim, Doaa, Elnagar, Alaa, Khatab, Awad, Manal A., Abdalla, Mohnad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635236/
https://www.ncbi.nlm.nih.gov/pubmed/34869059
http://dx.doi.org/10.3389/fcimb.2021.748739
_version_ 1784608262493044736
author Rasha, Elsayim
Alkhulaifi, Manal M.
AlOthman, Monerah
Khalid, Ibrahim
Doaa, Elnagar
Alaa, Khatab
Awad, Manal A.
Abdalla, Mohnad
author_facet Rasha, Elsayim
Alkhulaifi, Manal M.
AlOthman, Monerah
Khalid, Ibrahim
Doaa, Elnagar
Alaa, Khatab
Awad, Manal A.
Abdalla, Mohnad
author_sort Rasha, Elsayim
collection PubMed
description Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome carbapenem-resistant K. pneumoniae (KPC) in vitro and in vivo. ZnO-NPs were synthesized via a biological method and characterized using UV–Vis spectroscopy, Zetasizer and zeta potential analyses, x-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and energy-dispersive x-ray spectroscopy (EDX). In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a macro-dilution method. The morphological alteration of KPC cells after ZnO-NPs treatment was observed by SEM. The in vivo susceptibility of KPC cells to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The chemical characterization findings showed the formation, stability, shape, and size of the synthesized nanoparticles. The MIC and MBC were 0.7 and 1.8 mg/ml, respectively. The in vivo results displayed reduced inflammation and wound re-epithelialization of KPC-infected rats. These findings demonstrated that ZnO-NPs have great potential to be developed as antibacterial agents.
format Online
Article
Text
id pubmed-8635236
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86352362021-12-02 Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo Rasha, Elsayim Alkhulaifi, Manal M. AlOthman, Monerah Khalid, Ibrahim Doaa, Elnagar Alaa, Khatab Awad, Manal A. Abdalla, Mohnad Front Cell Infect Microbiol Cellular and Infection Microbiology Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome carbapenem-resistant K. pneumoniae (KPC) in vitro and in vivo. ZnO-NPs were synthesized via a biological method and characterized using UV–Vis spectroscopy, Zetasizer and zeta potential analyses, x-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and energy-dispersive x-ray spectroscopy (EDX). In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a macro-dilution method. The morphological alteration of KPC cells after ZnO-NPs treatment was observed by SEM. The in vivo susceptibility of KPC cells to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The chemical characterization findings showed the formation, stability, shape, and size of the synthesized nanoparticles. The MIC and MBC were 0.7 and 1.8 mg/ml, respectively. The in vivo results displayed reduced inflammation and wound re-epithelialization of KPC-infected rats. These findings demonstrated that ZnO-NPs have great potential to be developed as antibacterial agents. Frontiers Media S.A. 2021-11-16 /pmc/articles/PMC8635236/ /pubmed/34869059 http://dx.doi.org/10.3389/fcimb.2021.748739 Text en Copyright © 2021 Rasha, Alkhulaifi, AlOthman, Khalid, Doaa, Alaa, Awad and Abdalla https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Rasha, Elsayim
Alkhulaifi, Manal M.
AlOthman, Monerah
Khalid, Ibrahim
Doaa, Elnagar
Alaa, Khatab
Awad, Manal A.
Abdalla, Mohnad
Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title_full Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title_fullStr Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title_full_unstemmed Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title_short Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title_sort effects of zinc oxide nanoparticles synthesized using aspergillus niger on carbapenem-resistant klebsiella pneumonia in vitro and in vivo
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635236/
https://www.ncbi.nlm.nih.gov/pubmed/34869059
http://dx.doi.org/10.3389/fcimb.2021.748739
work_keys_str_mv AT rashaelsayim effectsofzincoxidenanoparticlessynthesizedusingaspergillusnigeroncarbapenemresistantklebsiellapneumoniainvitroandinvivo
AT alkhulaifimanalm effectsofzincoxidenanoparticlessynthesizedusingaspergillusnigeroncarbapenemresistantklebsiellapneumoniainvitroandinvivo
AT alothmanmonerah effectsofzincoxidenanoparticlessynthesizedusingaspergillusnigeroncarbapenemresistantklebsiellapneumoniainvitroandinvivo
AT khalidibrahim effectsofzincoxidenanoparticlessynthesizedusingaspergillusnigeroncarbapenemresistantklebsiellapneumoniainvitroandinvivo
AT doaaelnagar effectsofzincoxidenanoparticlessynthesizedusingaspergillusnigeroncarbapenemresistantklebsiellapneumoniainvitroandinvivo
AT alaakhatab effectsofzincoxidenanoparticlessynthesizedusingaspergillusnigeroncarbapenemresistantklebsiellapneumoniainvitroandinvivo
AT awadmanala effectsofzincoxidenanoparticlessynthesizedusingaspergillusnigeroncarbapenemresistantklebsiellapneumoniainvitroandinvivo
AT abdallamohnad effectsofzincoxidenanoparticlessynthesizedusingaspergillusnigeroncarbapenemresistantklebsiellapneumoniainvitroandinvivo