Cargando…

Divergent Response Strategies of CsABF Facing Abiotic Stress in Tea Plant: Perspectives From Drought-Tolerance Studies

In plants, the bZIP family plays vital roles in various biological processes, including seed maturation, flower development, light signal transduction, pathogen defense, and various stress responses. Tea, as a popular beverage, is widely cultivated and has withstood a degree of environmental adversi...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Jing, Du, Jinke, Tian, Liying, Li, Mengshuang, Zhang, Xianchen, Zhang, Shihua, Wan, Xiaochun, Chen, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635920/
https://www.ncbi.nlm.nih.gov/pubmed/34868162
http://dx.doi.org/10.3389/fpls.2021.763843
_version_ 1784608423829045248
author Lu, Jing
Du, Jinke
Tian, Liying
Li, Mengshuang
Zhang, Xianchen
Zhang, Shihua
Wan, Xiaochun
Chen, Qi
author_facet Lu, Jing
Du, Jinke
Tian, Liying
Li, Mengshuang
Zhang, Xianchen
Zhang, Shihua
Wan, Xiaochun
Chen, Qi
author_sort Lu, Jing
collection PubMed
description In plants, the bZIP family plays vital roles in various biological processes, including seed maturation, flower development, light signal transduction, pathogen defense, and various stress responses. Tea, as a popular beverage, is widely cultivated and has withstood a degree of environmental adversity. Currently, knowledge of the bZIP gene family in tea plants remains very limited. In this study, a total of 76 CsbZIP genes in tea plant were identified for the whole genome. Phylogenetic analysis with Arabidopsis counterparts revealed that CsbZIP proteins clustered into 13 subgroups, among which 13 ABFs related to the ABA signaling transduction pathway were further identified by conserved motif alignment and named CsABF1-13, these belonged to the A and S subgroups of CsbZIP and had close evolutionary relationships, possessing uniform or similar motif compositions. Transcriptome analysis revealed the expression profiles of CsABF genes in different tissues (bud, young leaf, mature leaf, old leaf, stem, root, flower, and fruit) and under diverse environmental stresses (drought, salt, chilling, and MeJA). Several CsABF genes with relatively low tissue expression, including CsABF1, CsABF5, CsABF9, and CsABF10, showed strong expression induction in stress response. Thirteen CsABF genes, were examined by qRT-PCR in two tea plant cultivars, drought-tolerant “Taicha 12” and drought-sensitive “Fuyun 6”, under exogenous ABA and drought stress. Furthermore, CsABF2, CsABF8, and CsABF11, were screened out as key transcription factors regulating drought tolerance of tea cultivars. Subsequently, some potential target genes regulated by CsABFs were screened by co-expression network and enrichment analysis. This study update CsbZIP gene family and provides a global survey of the ABF gene family in tea plant. The resolution of the molecular mechanism of drought resistance in different varieties could be helpful for improving stress resistance in tea plant via genetic engineering.
format Online
Article
Text
id pubmed-8635920
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86359202021-12-02 Divergent Response Strategies of CsABF Facing Abiotic Stress in Tea Plant: Perspectives From Drought-Tolerance Studies Lu, Jing Du, Jinke Tian, Liying Li, Mengshuang Zhang, Xianchen Zhang, Shihua Wan, Xiaochun Chen, Qi Front Plant Sci Plant Science In plants, the bZIP family plays vital roles in various biological processes, including seed maturation, flower development, light signal transduction, pathogen defense, and various stress responses. Tea, as a popular beverage, is widely cultivated and has withstood a degree of environmental adversity. Currently, knowledge of the bZIP gene family in tea plants remains very limited. In this study, a total of 76 CsbZIP genes in tea plant were identified for the whole genome. Phylogenetic analysis with Arabidopsis counterparts revealed that CsbZIP proteins clustered into 13 subgroups, among which 13 ABFs related to the ABA signaling transduction pathway were further identified by conserved motif alignment and named CsABF1-13, these belonged to the A and S subgroups of CsbZIP and had close evolutionary relationships, possessing uniform or similar motif compositions. Transcriptome analysis revealed the expression profiles of CsABF genes in different tissues (bud, young leaf, mature leaf, old leaf, stem, root, flower, and fruit) and under diverse environmental stresses (drought, salt, chilling, and MeJA). Several CsABF genes with relatively low tissue expression, including CsABF1, CsABF5, CsABF9, and CsABF10, showed strong expression induction in stress response. Thirteen CsABF genes, were examined by qRT-PCR in two tea plant cultivars, drought-tolerant “Taicha 12” and drought-sensitive “Fuyun 6”, under exogenous ABA and drought stress. Furthermore, CsABF2, CsABF8, and CsABF11, were screened out as key transcription factors regulating drought tolerance of tea cultivars. Subsequently, some potential target genes regulated by CsABFs were screened by co-expression network and enrichment analysis. This study update CsbZIP gene family and provides a global survey of the ABF gene family in tea plant. The resolution of the molecular mechanism of drought resistance in different varieties could be helpful for improving stress resistance in tea plant via genetic engineering. Frontiers Media S.A. 2021-11-17 /pmc/articles/PMC8635920/ /pubmed/34868162 http://dx.doi.org/10.3389/fpls.2021.763843 Text en Copyright © 2021 Lu, Du, Tian, Li, Zhang, Zhang, Wan and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lu, Jing
Du, Jinke
Tian, Liying
Li, Mengshuang
Zhang, Xianchen
Zhang, Shihua
Wan, Xiaochun
Chen, Qi
Divergent Response Strategies of CsABF Facing Abiotic Stress in Tea Plant: Perspectives From Drought-Tolerance Studies
title Divergent Response Strategies of CsABF Facing Abiotic Stress in Tea Plant: Perspectives From Drought-Tolerance Studies
title_full Divergent Response Strategies of CsABF Facing Abiotic Stress in Tea Plant: Perspectives From Drought-Tolerance Studies
title_fullStr Divergent Response Strategies of CsABF Facing Abiotic Stress in Tea Plant: Perspectives From Drought-Tolerance Studies
title_full_unstemmed Divergent Response Strategies of CsABF Facing Abiotic Stress in Tea Plant: Perspectives From Drought-Tolerance Studies
title_short Divergent Response Strategies of CsABF Facing Abiotic Stress in Tea Plant: Perspectives From Drought-Tolerance Studies
title_sort divergent response strategies of csabf facing abiotic stress in tea plant: perspectives from drought-tolerance studies
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635920/
https://www.ncbi.nlm.nih.gov/pubmed/34868162
http://dx.doi.org/10.3389/fpls.2021.763843
work_keys_str_mv AT lujing divergentresponsestrategiesofcsabffacingabioticstressinteaplantperspectivesfromdroughttolerancestudies
AT dujinke divergentresponsestrategiesofcsabffacingabioticstressinteaplantperspectivesfromdroughttolerancestudies
AT tianliying divergentresponsestrategiesofcsabffacingabioticstressinteaplantperspectivesfromdroughttolerancestudies
AT limengshuang divergentresponsestrategiesofcsabffacingabioticstressinteaplantperspectivesfromdroughttolerancestudies
AT zhangxianchen divergentresponsestrategiesofcsabffacingabioticstressinteaplantperspectivesfromdroughttolerancestudies
AT zhangshihua divergentresponsestrategiesofcsabffacingabioticstressinteaplantperspectivesfromdroughttolerancestudies
AT wanxiaochun divergentresponsestrategiesofcsabffacingabioticstressinteaplantperspectivesfromdroughttolerancestudies
AT chenqi divergentresponsestrategiesofcsabffacingabioticstressinteaplantperspectivesfromdroughttolerancestudies