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A Highly Sensitive GFP Activation Assay for Detection of DNA Cleavage in Cells

CRISPR/Cas9 nucleases hold great potential for gene therapy, but they frequently induce unwanted off-target cleavage. We previously developed a GFP activation assay for detection of DNA cleavage in cells. Here, we demonstrate two novel applications of this assay. First, we use this assay to confirm...

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Detalles Bibliográficos
Autores principales:	Hu, Ziying, Zhang, Chengdong, Wang, Daqi, Gao, Siqi, Ong, Sang-Ging, Wang, Yongming, Zheng, Wei V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Cell and Developmental Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636026/ https://www.ncbi.nlm.nih.gov/pubmed/34869366 http://dx.doi.org/10.3389/fcell.2021.771248

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