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Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks
DNA double strand breaks (DSBs) are among some of the most deleterious forms of DNA damage. Left unrepaired, they are detrimental to genome stability, leading to high risk of cancer. Two major mechanisms are responsible for the repair of DSBs, homologous recombination (HR) and nonhomologous end join...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636147/ https://www.ncbi.nlm.nih.gov/pubmed/34869333 http://dx.doi.org/10.3389/fcell.2021.745311 |
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author | Kong, Muwen Greene, Eric C. |
author_facet | Kong, Muwen Greene, Eric C. |
author_sort | Kong, Muwen |
collection | PubMed |
description | DNA double strand breaks (DSBs) are among some of the most deleterious forms of DNA damage. Left unrepaired, they are detrimental to genome stability, leading to high risk of cancer. Two major mechanisms are responsible for the repair of DSBs, homologous recombination (HR) and nonhomologous end joining (NHEJ). The complex nature of both pathways, involving a myriad of protein factors functioning in a highly coordinated manner at distinct stages of repair, lend themselves to detailed mechanistic studies using the latest single-molecule techniques. In avoiding ensemble averaging effects inherent to traditional biochemical or genetic methods, single-molecule studies have painted an increasingly detailed picture for every step of the DSB repair processes. |
format | Online Article Text |
id | pubmed-8636147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86361472021-12-02 Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks Kong, Muwen Greene, Eric C. Front Cell Dev Biol Cell and Developmental Biology DNA double strand breaks (DSBs) are among some of the most deleterious forms of DNA damage. Left unrepaired, they are detrimental to genome stability, leading to high risk of cancer. Two major mechanisms are responsible for the repair of DSBs, homologous recombination (HR) and nonhomologous end joining (NHEJ). The complex nature of both pathways, involving a myriad of protein factors functioning in a highly coordinated manner at distinct stages of repair, lend themselves to detailed mechanistic studies using the latest single-molecule techniques. In avoiding ensemble averaging effects inherent to traditional biochemical or genetic methods, single-molecule studies have painted an increasingly detailed picture for every step of the DSB repair processes. Frontiers Media S.A. 2021-11-15 /pmc/articles/PMC8636147/ /pubmed/34869333 http://dx.doi.org/10.3389/fcell.2021.745311 Text en Copyright © 2021 Kong and Greene. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Kong, Muwen Greene, Eric C. Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks |
title | Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks |
title_full | Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks |
title_fullStr | Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks |
title_full_unstemmed | Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks |
title_short | Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks |
title_sort | mechanistic insights from single-molecule studies of repair of double strand breaks |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636147/ https://www.ncbi.nlm.nih.gov/pubmed/34869333 http://dx.doi.org/10.3389/fcell.2021.745311 |
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