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CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma

Circular RNA (circNUP98) has been reported to promote renal cancer; however, its role in other cancers is unknown. The function of circNUP98 in glioblastoma (GB) cancer was explored in this study. A total of 58 GB tissue samples were collected to study the expression of circNUP98 and miR-519a-3p [bo...

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Autores principales: Lu, Jun, Lou, Gaojie, Jiang, Lin, Liu, Xiaoxing, Jiang, Jianxin, Wang, Xiaolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636316/
https://www.ncbi.nlm.nih.gov/pubmed/34867700
http://dx.doi.org/10.3389/fneur.2021.679745
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author Lu, Jun
Lou, Gaojie
Jiang, Lin
Liu, Xiaoxing
Jiang, Jianxin
Wang, Xiaolin
author_facet Lu, Jun
Lou, Gaojie
Jiang, Lin
Liu, Xiaoxing
Jiang, Jianxin
Wang, Xiaolin
author_sort Lu, Jun
collection PubMed
description Circular RNA (circNUP98) has been reported to promote renal cancer; however, its role in other cancers is unknown. The function of circNUP98 in glioblastoma (GB) cancer was explored in this study. A total of 58 GB tissue samples were collected to study the expression of circNUP98 and miR-519a-3p [both the mature and pre-mature microRNA (miRNA)] by quantitative real-time PCR (RT-qPCR) and heatmap analysis. The subcellular location that expresses circNUP98 was analyzed by nuclear fractionation assay. RNA pull-down assay was performed to evaluate the interaction between circNUP98 and pre-mature miR-519a-3p. Overexpression assays were performed to investigate the role of circNUP98 in the regulation of both the mature and pre-mature miR-519a-3p. The role of circNUP98 and miR-519a-3p in GB cell proliferation was explored by 5-bromo-2-deoxyuridine (BrdU) assay and was assessed in mouse xenograft model. Heatmap analysis showed that circNUP98 and pre-mature miR-519a-3p were upregulated in GB, while mature miR-519a-3p was downregulated in GB. Across the cancer tissues, circNUP98 was inversely correlated with mature miR-519a-3p, but positively correlated with pre-mature miR-519a-3p. In GB cells, circNUP98 was localized to both the nucleus and cytoplasm and it interacted with pre-mature miR-519a-3p. In GB cells, circNUP98 increased the expression levels of pre-mature miR-519a-3p and decreased the expression levels of mature miR-519a-3p. BrdU and cholecystokinin octapeptide (CCK-8) assays illustrated that overexpression of circNUP98 reduced the inhibitory effects of miR-519a-3p on cell proliferation. CircNUP98 contributed to larger tumors, which resulted in significantly reduced mice survival. CircNUP98 suppresses the maturation of miR-519a-3p to promote GB cell proliferation.
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spelling pubmed-86363162021-12-03 CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma Lu, Jun Lou, Gaojie Jiang, Lin Liu, Xiaoxing Jiang, Jianxin Wang, Xiaolin Front Neurol Neurology Circular RNA (circNUP98) has been reported to promote renal cancer; however, its role in other cancers is unknown. The function of circNUP98 in glioblastoma (GB) cancer was explored in this study. A total of 58 GB tissue samples were collected to study the expression of circNUP98 and miR-519a-3p [both the mature and pre-mature microRNA (miRNA)] by quantitative real-time PCR (RT-qPCR) and heatmap analysis. The subcellular location that expresses circNUP98 was analyzed by nuclear fractionation assay. RNA pull-down assay was performed to evaluate the interaction between circNUP98 and pre-mature miR-519a-3p. Overexpression assays were performed to investigate the role of circNUP98 in the regulation of both the mature and pre-mature miR-519a-3p. The role of circNUP98 and miR-519a-3p in GB cell proliferation was explored by 5-bromo-2-deoxyuridine (BrdU) assay and was assessed in mouse xenograft model. Heatmap analysis showed that circNUP98 and pre-mature miR-519a-3p were upregulated in GB, while mature miR-519a-3p was downregulated in GB. Across the cancer tissues, circNUP98 was inversely correlated with mature miR-519a-3p, but positively correlated with pre-mature miR-519a-3p. In GB cells, circNUP98 was localized to both the nucleus and cytoplasm and it interacted with pre-mature miR-519a-3p. In GB cells, circNUP98 increased the expression levels of pre-mature miR-519a-3p and decreased the expression levels of mature miR-519a-3p. BrdU and cholecystokinin octapeptide (CCK-8) assays illustrated that overexpression of circNUP98 reduced the inhibitory effects of miR-519a-3p on cell proliferation. CircNUP98 contributed to larger tumors, which resulted in significantly reduced mice survival. CircNUP98 suppresses the maturation of miR-519a-3p to promote GB cell proliferation. Frontiers Media S.A. 2021-11-18 /pmc/articles/PMC8636316/ /pubmed/34867700 http://dx.doi.org/10.3389/fneur.2021.679745 Text en Copyright © 2021 Lu, Lou, Jiang, Liu, Jiang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neurology
Lu, Jun
Lou, Gaojie
Jiang, Lin
Liu, Xiaoxing
Jiang, Jianxin
Wang, Xiaolin
CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title_full CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title_fullStr CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title_full_unstemmed CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title_short CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title_sort circnup98 suppresses the maturation of mir-519a-3p in glioblastoma
topic Neurology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636316/
https://www.ncbi.nlm.nih.gov/pubmed/34867700
http://dx.doi.org/10.3389/fneur.2021.679745
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