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Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China

BACKGROUND: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. OBJECTIVES: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. METH...

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Autores principales: Zhao, Ling, Zhang, Liang, Shi, Xiaona, Duan, Xujie, Li, Huiping, Liu, Shuying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636652/
https://www.ncbi.nlm.nih.gov/pubmed/34697919
http://dx.doi.org/10.4142/jvs.2021.22.e66
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author Zhao, Ling
Zhang, Liang
Shi, Xiaona
Duan, Xujie
Li, Huiping
Liu, Shuying
author_facet Zhao, Ling
Zhang, Liang
Shi, Xiaona
Duan, Xujie
Li, Huiping
Liu, Shuying
author_sort Zhao, Ling
collection PubMed
description BACKGROUND: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. OBJECTIVES: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. METHODS: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. RESULTS: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%–86.8% and 67.7%–75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). CONCLUSIONS: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.
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spelling pubmed-86366522021-12-13 Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China Zhao, Ling Zhang, Liang Shi, Xiaona Duan, Xujie Li, Huiping Liu, Shuying J Vet Sci Original Article BACKGROUND: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. OBJECTIVES: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. METHODS: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. RESULTS: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%–86.8% and 67.7%–75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). CONCLUSIONS: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library. The Korean Society of Veterinary Science 2021-11 2021-08-03 /pmc/articles/PMC8636652/ /pubmed/34697919 http://dx.doi.org/10.4142/jvs.2021.22.e66 Text en © 2021 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zhao, Ling
Zhang, Liang
Shi, Xiaona
Duan, Xujie
Li, Huiping
Liu, Shuying
Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China
title Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China
title_full Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China
title_fullStr Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China
title_full_unstemmed Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China
title_short Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China
title_sort next-generation sequencing for the genetic characterization of maedi/visna virus isolated from the northwest of china
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636652/
https://www.ncbi.nlm.nih.gov/pubmed/34697919
http://dx.doi.org/10.4142/jvs.2021.22.e66
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