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Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd

Germplasm collections are indispensable resources for the mining of important genes and variety improvement. To preserve and utilize germplasm collections in bottle gourd, we identified and validated a highly informative core single-nucleotide polymorphism (SNP) marker set from 1,100 SNPs. This mark...

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Autores principales: Wang, Ying, Wu, Xiaohua, Li, Yanwei, Feng, Zishan, Mu, Zihan, Wang, Jiang, Wu, Xinyi, Wang, Baogen, Lu, Zhongfu, Li, Guojing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636714/
https://www.ncbi.nlm.nih.gov/pubmed/34868131
http://dx.doi.org/10.3389/fpls.2021.747940
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author Wang, Ying
Wu, Xiaohua
Li, Yanwei
Feng, Zishan
Mu, Zihan
Wang, Jiang
Wu, Xinyi
Wang, Baogen
Lu, Zhongfu
Li, Guojing
author_facet Wang, Ying
Wu, Xiaohua
Li, Yanwei
Feng, Zishan
Mu, Zihan
Wang, Jiang
Wu, Xinyi
Wang, Baogen
Lu, Zhongfu
Li, Guojing
author_sort Wang, Ying
collection PubMed
description Germplasm collections are indispensable resources for the mining of important genes and variety improvement. To preserve and utilize germplasm collections in bottle gourd, we identified and validated a highly informative core single-nucleotide polymorphism (SNP) marker set from 1,100 SNPs. This marker set consisted of 22 uniformly distributed core SNPs with abundant polymorphisms, which were established to have strong representativeness and discriminatory power based on analyses of 206 bottle gourd germplasm collections and a multiparent advanced generation inter-cross (MAGIC) population. The core SNP markers were used to assess genetic diversity and population structure, and to fingerprint important accessions, which could provide an optimized procedure for seed authentication. Furthermore, using the core SNP marker set, we developed an accessible core population of 150 accessions that represents 100% of the genetic variation in bottle gourds. This core population will make an important contribution to the preservation and utilization of bottle gourd germplasm collections, cultivar identification, and marker-assisted breeding.
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spelling pubmed-86367142021-12-03 Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd Wang, Ying Wu, Xiaohua Li, Yanwei Feng, Zishan Mu, Zihan Wang, Jiang Wu, Xinyi Wang, Baogen Lu, Zhongfu Li, Guojing Front Plant Sci Plant Science Germplasm collections are indispensable resources for the mining of important genes and variety improvement. To preserve and utilize germplasm collections in bottle gourd, we identified and validated a highly informative core single-nucleotide polymorphism (SNP) marker set from 1,100 SNPs. This marker set consisted of 22 uniformly distributed core SNPs with abundant polymorphisms, which were established to have strong representativeness and discriminatory power based on analyses of 206 bottle gourd germplasm collections and a multiparent advanced generation inter-cross (MAGIC) population. The core SNP markers were used to assess genetic diversity and population structure, and to fingerprint important accessions, which could provide an optimized procedure for seed authentication. Furthermore, using the core SNP marker set, we developed an accessible core population of 150 accessions that represents 100% of the genetic variation in bottle gourds. This core population will make an important contribution to the preservation and utilization of bottle gourd germplasm collections, cultivar identification, and marker-assisted breeding. Frontiers Media S.A. 2021-11-18 /pmc/articles/PMC8636714/ /pubmed/34868131 http://dx.doi.org/10.3389/fpls.2021.747940 Text en Copyright © 2021 Wang, Wu, Li, Feng, Mu, Wang, Wu, Wang, Lu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Ying
Wu, Xiaohua
Li, Yanwei
Feng, Zishan
Mu, Zihan
Wang, Jiang
Wu, Xinyi
Wang, Baogen
Lu, Zhongfu
Li, Guojing
Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd
title Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd
title_full Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd
title_fullStr Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd
title_full_unstemmed Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd
title_short Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd
title_sort identification and validation of a core single-nucleotide polymorphism marker set for genetic diversity assessment, fingerprinting identification, and core collection development in bottle gourd
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636714/
https://www.ncbi.nlm.nih.gov/pubmed/34868131
http://dx.doi.org/10.3389/fpls.2021.747940
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