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Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd
Germplasm collections are indispensable resources for the mining of important genes and variety improvement. To preserve and utilize germplasm collections in bottle gourd, we identified and validated a highly informative core single-nucleotide polymorphism (SNP) marker set from 1,100 SNPs. This mark...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636714/ https://www.ncbi.nlm.nih.gov/pubmed/34868131 http://dx.doi.org/10.3389/fpls.2021.747940 |
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author | Wang, Ying Wu, Xiaohua Li, Yanwei Feng, Zishan Mu, Zihan Wang, Jiang Wu, Xinyi Wang, Baogen Lu, Zhongfu Li, Guojing |
author_facet | Wang, Ying Wu, Xiaohua Li, Yanwei Feng, Zishan Mu, Zihan Wang, Jiang Wu, Xinyi Wang, Baogen Lu, Zhongfu Li, Guojing |
author_sort | Wang, Ying |
collection | PubMed |
description | Germplasm collections are indispensable resources for the mining of important genes and variety improvement. To preserve and utilize germplasm collections in bottle gourd, we identified and validated a highly informative core single-nucleotide polymorphism (SNP) marker set from 1,100 SNPs. This marker set consisted of 22 uniformly distributed core SNPs with abundant polymorphisms, which were established to have strong representativeness and discriminatory power based on analyses of 206 bottle gourd germplasm collections and a multiparent advanced generation inter-cross (MAGIC) population. The core SNP markers were used to assess genetic diversity and population structure, and to fingerprint important accessions, which could provide an optimized procedure for seed authentication. Furthermore, using the core SNP marker set, we developed an accessible core population of 150 accessions that represents 100% of the genetic variation in bottle gourds. This core population will make an important contribution to the preservation and utilization of bottle gourd germplasm collections, cultivar identification, and marker-assisted breeding. |
format | Online Article Text |
id | pubmed-8636714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86367142021-12-03 Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd Wang, Ying Wu, Xiaohua Li, Yanwei Feng, Zishan Mu, Zihan Wang, Jiang Wu, Xinyi Wang, Baogen Lu, Zhongfu Li, Guojing Front Plant Sci Plant Science Germplasm collections are indispensable resources for the mining of important genes and variety improvement. To preserve and utilize germplasm collections in bottle gourd, we identified and validated a highly informative core single-nucleotide polymorphism (SNP) marker set from 1,100 SNPs. This marker set consisted of 22 uniformly distributed core SNPs with abundant polymorphisms, which were established to have strong representativeness and discriminatory power based on analyses of 206 bottle gourd germplasm collections and a multiparent advanced generation inter-cross (MAGIC) population. The core SNP markers were used to assess genetic diversity and population structure, and to fingerprint important accessions, which could provide an optimized procedure for seed authentication. Furthermore, using the core SNP marker set, we developed an accessible core population of 150 accessions that represents 100% of the genetic variation in bottle gourds. This core population will make an important contribution to the preservation and utilization of bottle gourd germplasm collections, cultivar identification, and marker-assisted breeding. Frontiers Media S.A. 2021-11-18 /pmc/articles/PMC8636714/ /pubmed/34868131 http://dx.doi.org/10.3389/fpls.2021.747940 Text en Copyright © 2021 Wang, Wu, Li, Feng, Mu, Wang, Wu, Wang, Lu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Wang, Ying Wu, Xiaohua Li, Yanwei Feng, Zishan Mu, Zihan Wang, Jiang Wu, Xinyi Wang, Baogen Lu, Zhongfu Li, Guojing Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd |
title | Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd |
title_full | Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd |
title_fullStr | Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd |
title_full_unstemmed | Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd |
title_short | Identification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment, Fingerprinting Identification, and Core Collection Development in Bottle Gourd |
title_sort | identification and validation of a core single-nucleotide polymorphism marker set for genetic diversity assessment, fingerprinting identification, and core collection development in bottle gourd |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636714/ https://www.ncbi.nlm.nih.gov/pubmed/34868131 http://dx.doi.org/10.3389/fpls.2021.747940 |
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