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Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips

The accurate identification of mastitis‐causing bacteria assists in effective management by both dairy farmers and veterinarians and can be used to implement the selective use of antimicrobials for treatment. The purpose of this study was to evaluate the ability of our developed anti–ribosomal prote...

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Autores principales: KIKU, Yoshio, NAGASAWA, Yuya, SUGAWARA, Kazue, YABUSAKI, Takahiro, OONO, Kazuyoshi, FUJII, Kento, MAEHANA, Koji, HAYASHI, Tomohito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636885/
https://www.ncbi.nlm.nih.gov/pubmed/34526418
http://dx.doi.org/10.1292/jvms.21-0185
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author KIKU, Yoshio
NAGASAWA, Yuya
SUGAWARA, Kazue
YABUSAKI, Takahiro
OONO, Kazuyoshi
FUJII, Kento
MAEHANA, Koji
HAYASHI, Tomohito
author_facet KIKU, Yoshio
NAGASAWA, Yuya
SUGAWARA, Kazue
YABUSAKI, Takahiro
OONO, Kazuyoshi
FUJII, Kento
MAEHANA, Koji
HAYASHI, Tomohito
author_sort KIKU, Yoshio
collection PubMed
description The accurate identification of mastitis‐causing bacteria assists in effective management by both dairy farmers and veterinarians and can be used to implement the selective use of antimicrobials for treatment. The purpose of this study was to evaluate the ability of our developed anti–ribosomal protein-L7/L12 antibody–coated immunochromatographic strip (ICS) test to detect coliforms in milk by comparing the results with the bacteriological culture method. We investigated the performance of the ICS test as compared with the bacteriological culture method using 308 milk samples from clinical bovine mastitis. First, to determine the optimal ICS test cutoff point for detecting coliform mastitis, we developed a receiver-operating characteristic curve. The result showed that the cutoff point was at 0.5 of our index. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value of the ICS test were 81.3%, 84.8%, 69.2%, and 91.54%, respectively. As the clinical signs increased in severity, the F-measure, a weighted harmonic mean of the sensitivity and overall PPV performance, increased. Because it is especially important to treat clinical mastitis appropriately in the early stages of detection, the ICS test, which can be used by both dairy farmers and veterinarians on dairy farms, is considered to be a useful tool for detecting coliform mastitis, which often presents with severe signs.
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spelling pubmed-86368852021-12-07 Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips KIKU, Yoshio NAGASAWA, Yuya SUGAWARA, Kazue YABUSAKI, Takahiro OONO, Kazuyoshi FUJII, Kento MAEHANA, Koji HAYASHI, Tomohito J Vet Med Sci Internal Medicine The accurate identification of mastitis‐causing bacteria assists in effective management by both dairy farmers and veterinarians and can be used to implement the selective use of antimicrobials for treatment. The purpose of this study was to evaluate the ability of our developed anti–ribosomal protein-L7/L12 antibody–coated immunochromatographic strip (ICS) test to detect coliforms in milk by comparing the results with the bacteriological culture method. We investigated the performance of the ICS test as compared with the bacteriological culture method using 308 milk samples from clinical bovine mastitis. First, to determine the optimal ICS test cutoff point for detecting coliform mastitis, we developed a receiver-operating characteristic curve. The result showed that the cutoff point was at 0.5 of our index. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value of the ICS test were 81.3%, 84.8%, 69.2%, and 91.54%, respectively. As the clinical signs increased in severity, the F-measure, a weighted harmonic mean of the sensitivity and overall PPV performance, increased. Because it is especially important to treat clinical mastitis appropriately in the early stages of detection, the ICS test, which can be used by both dairy farmers and veterinarians on dairy farms, is considered to be a useful tool for detecting coliform mastitis, which often presents with severe signs. The Japanese Society of Veterinary Science 2021-09-15 2021-11 /pmc/articles/PMC8636885/ /pubmed/34526418 http://dx.doi.org/10.1292/jvms.21-0185 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Internal Medicine
KIKU, Yoshio
NAGASAWA, Yuya
SUGAWARA, Kazue
YABUSAKI, Takahiro
OONO, Kazuyoshi
FUJII, Kento
MAEHANA, Koji
HAYASHI, Tomohito
Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips
title Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips
title_full Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips
title_fullStr Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips
title_full_unstemmed Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips
title_short Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips
title_sort evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636885/
https://www.ncbi.nlm.nih.gov/pubmed/34526418
http://dx.doi.org/10.1292/jvms.21-0185
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